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Root Exudates Response of Mycorrhizal Pinus massoniana Seedlings Under Drought Stress

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Abstract

Pinus massoniana Lamb. (P. massoniana) grown under water-deficient conditions displays a series of coordinated adaptive responses. Although ectomycorrhizal (ECM) fungi can enhance plant adaptation to water deficiency, the research of ECM fungi colonization and water stress intensity induce P. massoniana seedlings root exudates to resist is no depth. One-year-old P. massoniana seedlings without inoculation (St1) and inoculated with Suillus luteus (St2, St3) were studied under no water stress 70–80% (Wt1), mild stress 60–70% (Wt2), moderate stress 50–60% (Wt3), and severe stress 35–50% (Wt4), respectively. The root exudates of seedlings were extracted by organic extraction method and identified by gas chromatography-mass spectrometry (GC–MS). The results show that root exudates variety and contents of different seedlings decreased as drought stress increased. Except for St1 under Wt1 treatment, other seedlings root secreted mono (2-ethylhexyl) phthalate (MEHP). Under the same water treatment, MEHP content of seedlings was Wt2: St1 (17.74%) < St3 (21.04%) < St2 (23.67%), Wt3: St1 (76.46%) < St2 (77.35%) < St3 (77.82%), and Wt4: St1 (0%) < St3 (56.71%) < St2 (61.65%). The content of MEHP in mycorrhizal P. massoniana seedlings was higher than that of un-inoculated seedlings. Therefore, drought can reduce root exudates richness of P. massoniana seedlings and the more obvious difference was that ECM fungi can increase the content of MEHP in seedlings significantly. The change was likely an important feature of mycorrhiza P. massoniana seedlings to adapt to drought stress.

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Data Availability

The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We would like to thank Professor Guijie Ding of Guizhou University and Associate Professor Yi Wang of Guizhou University for Nationalities for their support and help to the project. The strains were provided by Professor Jianguo Huang of Southwest University. The excellent forest stands in Duyun City, Guizhou Province, provided P. massoniana seeds.

Funding

This research was supported by the National Natural Science Foundation (NSFC) (32360379); Guizhou Provincial Basic Research Program of Natural Science (qjhe-ZK[2022]General207); Youth Scientific and Technological Talent Growth Project of Guizhou Provincial Department of Education (qjhe KY [2018]134); and the Natural Science Foundation of Guizhou Minzu University (gzmu [2019] yb18).

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Authors and Affiliations

  1. College of Eco-Environmental Engineering, Guizhou Minzu University, Guiyang, 550025, China

    Yi Wang & Qina Chen

  2. Agro-Environmental Protection Institute Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Tianjing, 300191, China

    He Hu

  3. Department of Renewable Resources, University of Alberta, 442 Earth Sciences Building, Edmonton, T6G 2E3, Canada

    Wenqing Zhang

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  1. Yi Wang
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YW designed the research, provided guidance, and reviewed the manuscript; QC analyzed data, drew the drawings, and then wrote and revised the manuscript; HH performed the experiments and collected data; WZ assisted in the experiments and manuscript. All authors read and approved the final manuscript.

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Correspondence to Yi Wang.

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Wang, Y., Chen, Q., Hu, H. et al. Root Exudates Response of Mycorrhizal Pinus massoniana Seedlings Under Drought Stress. J Plant Growth Regul 43, 576–588 (2024). https://doi.org/10.1007/s00344-023-11115-8

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