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Microbial Volatiles (mVOCs) Induce Tomato Plant Growth and Disease Resistance Against Wilt Pathogen Fusarium oxysporum f.sp. lycopersici

Journal of Plant Growth Regulation (2023)Cite this article

Abstract

Microbial volatile organic compounds (mVOCs) and its potentiality in plant growth and development is still an unexplored area. The in vitro study on bipartite plate showed inhibition of Fusarium oxysporum f.sp lycopersici (FOL) by volatiles of Trichoderma asperellum BHU P1 and Ochrobactrum sp. BHU-PB1 over control. The seed germination and seedling growth was recorded maximum in plant exposed to VOCs of Ochrobactrum sp. in both magenta box (in vitro) and pot (in vivo) experiment. The growth parameters as seed germination, shoot length, root length, fresh weight, dry weight, number of lateral roots and number of leaflets was compared to be higher in microbial volatile metabolites treated plants as compared to control. Disease incidence in T. asperellum and Ochrobactrum sp. volatile treated tomato plants were 43.66% and 41.33%, respectively at 20 days post inoculation (dpi) whereas untreated control showed disease incidence up to 79.16%. GC–MS analysis of volatile metabolite of T. asperellum BHU P1 detected 42 compounds and Ochrobactrum sp. BHU-PB1 detected 50 compounds over PDB and NB control. HPLC analysis of volatile treated tomato leaves showed enhanced concentration of gallic acid, t-chlorogenic acid, rutin, p-caumeric acid, cinnamic acid, ferulic acid, capsacin, salicylic acid, syringic acid and quercetin over control at 0, 48 and 72 h of FOL challenge. Above observations led to the conclusion that tomato roots which perceived the volatiles of Ochrobactrum sp. BHU-PB1 showed better plant growth promotion and enhanced plant defense, revealed by higher phenolic compound production followed by T. asperellum BHU P1 volatile treated plant.

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Acknowledgements

P. Singh is grateful to UGC-RET fellowship for providing financial assistance. J. Singh is thankful to CSIR-JRF fellowship. A. Vaishnav wants to acknowledge SERB-NPDF (PDF/2017/000689) for providing financial assistance.

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Authors and Affiliations

  1. Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221005, India

    Prachi Singh & Rakesh Kumar Singh

  2. Department of Botany, Banaras Hindu University, Varanasi, 221005, India

    Jyoti Singh

  3. Agricultural Research Organization, Volcani Centre, Rishon LeZion, Israel

    Shatrupa Ray

  4. Department of Biotechnology, GLA University, Mathura, 281406, Uttar Pradesh, India

    Anukool Vaishnav & Harikesh Bahadur Singh

  5. Rabindranath Tagore Agriculture College, Deoghar, Birsa Agriculture University, Ranchi, 834006, Jharkhand, India

    Prachi Singh

  6. Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, India

    Priyanka Jha

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  1. Prachi Singh
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  2. Jyoti Singh
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  4. Anukool Vaishnav
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Contributions

PS and JS performed experiments and drafted first manuscript. AV designed the experiments and edited final manuscript. SR edited first draft and gave valuable suggestion during this research. PJ worked on reviewer's comments and edited revised manuscript. RKS and HBS supervised coordinated overall work and gave valuable comments.

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Correspondence to Anukool Vaishnav or Harikesh Bahadur Singh.

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Singh, P., Singh, J., Ray, S. et al. Microbial Volatiles (mVOCs) Induce Tomato Plant Growth and Disease Resistance Against Wilt Pathogen Fusarium oxysporum f.sp. lycopersici. J Plant Growth Regul (2023). https://doi.org/10.1007/s00344-023-11060-6

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