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Transcriptome Analysis Revealed the Differential Molecular Response Mechanisms of Roots, Stems, and Leaves in Mung Bean to Cadmium Stress

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Abstract

To acquire a comprehensive understanding of the different plant organ molecular mechanisms in response to cadmium (Cd), transcriptome analyses of mung bean roots, stems, and leaves exposed to 100-μM Cd for 1, 5, and 9 days were performed. A total of 13,055, 8189, and 6705 differentially expressed genes (DEGs) were obtained in roots, stems, and leaves, respectively. The analysis of DEG enrichment and functional profiling showed that the upregulated DEGs in roots were significantly (p < 0.05) enriched in signaling-, oxidation‒reduction-, and detoxification process-related GO terms, and the downregulated DEGs in roots were significantly (p < 0.05) enriched in cell wall-, membrane-, and cell cytoskeleton-related GO terms. The upregulated DEGs in stems were significantly (p < 0.05) enriched in signaling-, detoxification process-, and secondary metabolic process-related GO terms, and the downregulated DEGs in stems were significantly (p < 0.05) enriched in cell wall- and membrane-related GO terms. The upregulated DEGs in leaves were significantly (p < 0.05) enriched in regulation of transcription- and oxidation‒reduction process-related GO terms, and the downregulated DEGs in leaves were significantly (p < 0.05) enriched in cell cytoskeleton- and photosynthesis-related GO terms. Key DEGs involved in the Cd response were obtained, including glutathione S-transferase-coding DEGs, pathogenesis-related protein-coding DEGs, isocitrate lyase-coding DEGs, malate synthase-coding DEGs, peroxidase-coding DEGs, photosynthesis-related DEGs, transporter-related DEGs, and secondary metabolism-related DEGs. Based on the results, it was proposed that the root was the principal organ involved in the response to Cd stress, the antioxidant system and secondary metabolic process were the main response pathways of the stem in response to Cd, and photosynthesis and the glyoxylate pathway were important biological processes in the leaf response to Cd.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (3176110).

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Authors and Affiliations

  1. School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, China

    Ping-min Wu & Shi-Weng Li

  2. School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, China

    Yan Leng, Yu-Lin Wang & Fu-Jun Qiao

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  1. Ping-min Wu
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Contributions

LSW conceived of the study, participated in the design of experiment, and analysis of the experimental data. WPM and LY performed the experiments and statistical analysis and drafted the manuscript. WYL and QFJ participated in the culture and sampling of experimental materials. All authors read and approved the final manuscript.

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Correspondence to Shi-Weng Li.

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Wu, Pm., Leng, Y., Li, SW. et al. Transcriptome Analysis Revealed the Differential Molecular Response Mechanisms of Roots, Stems, and Leaves in Mung Bean to Cadmium Stress. J Plant Growth Regul 42, 7054–7069 (2023). https://doi.org/10.1007/s00344-023-10997-y

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