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Determination of Zn Responsive Genes Involved in Zn Fertilization in Peanuts Based on Transcriptome Analysis

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Abstract

Zn fertilizer is extensively applied in agricultural practices, which promotes photosynthetic process and growth development; however, Zn fertilizer use efficiency is very low in main crops, and studies on Zn responsive genes (ZRGs) are still lacking. Here, we used transcriptome analysis on peanuts with two Zn soluble resources (ZnSO4.7H2O and ZnCl2) to dissect the ZRGs. Results show that the two Zn resources had significantly positive effects on photosynthetic parameters, including net photosynthetic rates, chlorophyll contents, and photochemical efficiency. Results from transcriptomes on peanuts with different Zn treatments reveal that 862 and 947 genes were upregulated by ZnCl2 (+ 0.1% v/v) and ZnSO4.7H2O (+ 0.3% v/v), relative to the control, respectively. GO and KEGG analysis shows that zinc binding, signal transduction, cell membrane biosynthesis, ion/carbohydrate transport, and amino acids metabolism were significantly enriched in 368 ZRGs, while the top 5% ZRGs with extremely differential expression were further confirmed by qPCR. Results confirm that the genes related to Zn binding and photosynthetic process were upregulated and genes related to defense system were regulated by both Zn treatments. This study reveals multiple signal pathways involved in Zn ion response and emphasizes promoted roles of ion transport and carbohydrate metabolism in Zn fertilizer use efficiency for peanut growth.

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Acknowledgements

This work was supported by Natural Science Foundation of Zhejiang Province (Grant No: LQ20C130003) and Scientific Research Fund of Zhejiang Provincial Education Department (Y202248468). We thank Zhongkang Omics Ltd. Corp. for technical service on transcriptome analysis.

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Authors and Affiliations

  1. Department of Life Sciences and Health, Huzhou College, 1 Xueshi Road, Wuxing, Huzhou, 313000, Zhejiang, China

    Sushuang Liu, Chundong Liu & Yanmin Liu

  2. College of Life Science, Huzhou University, 1 Xueshi Road, Wuxing, Huzhou, 313000, Zhejiang, China

    Yang Li

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  1. Sushuang Liu
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  2. Chundong Liu
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  3. Yang Li
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  4. Yanmin Liu
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Contributions

Conceptualization, YML.; Methodology, SL, CL; Investigation, SL, CL, YL; Statistical analysis, SL, CL, YL; Writing, YML, YL; Funding Acquisition, CL, SL Resources, CL, Supervision, YML.

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Correspondence to Yanmin Liu.

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Liu, S., Liu, C., Li, Y. et al. Determination of Zn Responsive Genes Involved in Zn Fertilization in Peanuts Based on Transcriptome Analysis. J Plant Growth Regul 42, 3162–3172 (2023). https://doi.org/10.1007/s00344-022-10781-4

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  • DOI: https://doi.org/10.1007/s00344-022-10781-4

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