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Riboflavin Seed Priming Activates OsNHXs Expression to Alleviate Salinity Stress in Rice Seedlings

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Abstract

Rice possesses well-developed mechanisms of managing excessive sodium concentration under salinity stress condition. However, such mechanisms are limited in sensitive rice varieties. In the present study, we elucidated the role of riboflavin (RIB, vitamin B2) in ameliorating salinity stress in a well-known salt-sensitive rice cultivar (Oryza sativa L. cv. Koshihikari). Rice seeds were soaked in 10-μM RIB solution for 24 h, air-dried, and then the germinated seeds were cultivated hydroponically for three weeks under 25-mM NaCl to stimulate salinity stress prior to harvest. RIB-treated seedlings exhibited higher biomass accumulation, higher Sodium (Na+) and Potassium (K+) concentrations in the roots, and higher K+ concentration in the leaf blades which led to lower Na+/K+ ratio in the leaf blades. Also, RIB-treated seedlings possessed higher Calcium (Ca), Magnesium (Mg), and Copper (Cu) concentrations in the leaf blades and Iron (Fe) concentration in the roots compared to the non-treated seedlings under salinity stress. The results may be attributed to alterations in the transcript levels of Na+ transporter-encoding genes (OsHKT2;1, OsNHX1, OsNHX2, OsNHX4, OsNHX5) in the roots and leaf blades and the halotolerance gene (OsHAL3) in the leaf blades. The results indicate that RIB-treated seedlings positively regulate Na+ and K+ uptake, in turn, enhancing tissue tolerance mechanisms of alleviating salinity stress in salt-sensitive rice cultivar.

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Acknowledgements

This research was supported by JSPS KAKENHI Grant Number 20KK0129 to AU.

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Authors and Affiliations

  1. Graduate School of Integrated Sciences for Life, Hiroshima University, 1-4-4 Kagamiyama,, Higashi-Hiroshima, Hiroshima, 739-8528, Japan

    Kamonthip Jiadkong, Mami Nampei & Akihiro Ueda

  2. Bodhivijjalaya College, Srinakharinwirot University, No.63 M.7 Rangsit-Nakhon Nayok Rd. Ongkharak, Nakhon Nayok, 26120, Thailand

    Sumana Wangsawang

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  1. Kamonthip Jiadkong
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The authors have made the following declarations about their contributions. KJ, SW, and AU contributed to conception and design the experiments. KJ, MN, and SW performed the experiments and collected the data. KJ and MN contributed to analysis and data processing. KJ and AU contributed to writing and manuscript processing.

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Correspondence to Akihiro Ueda.

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Jiadkong, K., Nampei, M., Wangsawang, S. et al. Riboflavin Seed Priming Activates OsNHXs Expression to Alleviate Salinity Stress in Rice Seedlings. J Plant Growth Regul 42, 3032–3042 (2023). https://doi.org/10.1007/s00344-022-10768-1

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