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The Negative Effect of Coinoculation of Plant Growth-Promoting Bacteria Is Not Related to Indole-3-Acetic Acid Synthesis

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Abstract

The use of coinoculations of indole-3-acetic acid-synthesizing bacteria has become increasingly common in agriculture. Different bacterial isolates are capable of synthesizing IAA. Depending on the amount, IAA can promote, inhibit, or modify the growth and development of plants. However, little is known about the effect of auxins on plants under coinoculation conditions. The present study aimed to verify whether coinoculation with indole-3-acetic acid (IAA)-synthesizing bacteria may or may not harm plant development in three tomato genotypes with different sensitivities to auxins: Micro-Tom (MT), diageotropica (dgt) (low auxin sensitivity) and Entire (high auxin biosynthesis). The experiment was conducted in a completely randomized design with six replicates and eight treatments: control; Bacillus velezensis (BV); B. pumilus (BP); Azospirillum brasilense (AZ); B. velezensis + Bacillus pumilus (BV + BP); B. velezensis + A brasilense (BV + AZ); B. pumilus + A brasilense (BP + AZ); B. velezensis + Bacillus pumilus + A. brasilense (BV + BP + AZ), for each tomato genotype. The parameters analyzed were dry shoot and root weight, plant height, and leaf area. The results showed an increase in root dry weight with BV + BP + AZ treatment in the MT genotype, an increase in shoot dry weight in the dgt genotype with BV and no increase in any parameter in the Entire genotype for the analyzed parameters. The results also showed that coinoculation with BP + AZ decreased the shoot dry weight, dry root weight, and leaf area values of the dgt and Entire genotypes. Coinoculations with BP + AZ reduced the parameters analyzed in the dgt genotype, which is insensitive to the IAA and Entire genotypes and is sensitive. In addition, coinoculation with BV + BP + AZ, which provided the highest IAA concentration, increased the dry root weight in the MT genotype. These results suggest that the reduction in plant development caused by coinoculation is not related to IAA synthesis produced by bacteria but is probably due to negative interference in the interaction mechanisms between plants and bacteria and might also be due to negative bacteria–bacteria interactions.

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Funding

This study was supported by capes (Grant No. 001) to Laiana Lana Bentes Lobo

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Authors and Affiliations

  1. Graduate Program in Agricultural Microbiology, Faculty of Agrarian and Veterinary Sciences, State University of São Paulo (UNESP), Jaboticabal, Brazil

    Laiana Lana Bentes Lobo, Maura Santos Reis de Andrade da Silva & Everlon Cid Rigobelo

  2. Department of Applied Biology to Agriculture, Faculty of Agrarian and Veterinary Sciences, Access Route Prof. Paulo Donato Castelane, Vila Industrial, Jaboticabal, S/N, 14884-900, Brazil

    Rogério Falleiros Carvalho

Authors
  1. Laiana Lana Bentes Lobo
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  2. Maura Santos Reis de Andrade da Silva
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  3. Rogério Falleiros Carvalho
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  4. Everlon Cid Rigobelo
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Contributions

Laiana Lana Bentes Lobo executed the trial and analyzed the results. Maura Santos dos Reis Andrade da Silva made the graphics. Rogério Falleiros Carvalho and Everlon Cid Rigobelo corrected the text.

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Correspondence to Everlon Cid Rigobelo.

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Lobo, L.L.B., da Silva, M.S.R.d., Carvalho, R.F. et al. The Negative Effect of Coinoculation of Plant Growth-Promoting Bacteria Is Not Related to Indole-3-Acetic Acid Synthesis. J Plant Growth Regul 42, 2317–2326 (2023). https://doi.org/10.1007/s00344-022-10706-1

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