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Effects of Autotoxicity on Seed Germination, Gas Exchange Attributes and Chlorophyll Fluorescence in Melon Seedlings

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Abstract

In recent years, continuous cropping is more and more common in melon production, which shows significant adverse effects on melon cultivation. Autotoxicity is a critical factor for continuous cropping obstacle. The purpose of this study was to investigate whether the changes of chlorophyll fluorescence parameters could sensitively reflect the degree of melon autotoxicity and its damage. The root aqueous extract of melon plant was used to simulate the autotoxicity. The effects of autotoxicity on seed germination, seedling morphology, photosynthesis and chlorophyll a fluorescence transient (OJIP) were determined. The results showed that autotoxicity significantly inhibited seed germination and subsequent growth. The content of chlorophyll and carotenoid, photosynthetic rate, stomatal conductance, water-use efficiency and transpiration rate decreased significantly in melon seedlings under autotoxicity. The OJIP test parameters showed slight improvement or no obvious change at low treatment concentration (0.01 and 0.02 g·mL−1) and deteriorated rapidly at high concentration (0.03 and 0.04 g·mL−1), which was consistent with the concentration-dependent characteristics of autotoxicity. Under high autotoxicity stress concentration, most parameters related specific fluxes or activities per photosystem II (PS II) reaction centers (RC), such as absorption flux per active RC (ABS/RC), dissipated energy flux per active RC (DIo/RC) and other parameters, increased significantly in OJIP test. At the same time, most parameters related yields or flux ratios and performance index, such as maximum quantum yield of primary photochemistry (φPo), performance index on absorption basis (PIabs) and other parameters, decreased significantly. Photoinhibition induced by autotoxicity affected both the donor and the acceptor side of PS II, and then inhibited the growth of plants, decreased of photosynthetic pigment content. OJIP test parameters can be used to measure effectively the effect of autotoxicity on melon seedlings.

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References

  • Aguiar BDM, Vida JB, Tessmann DJ, Oliveira RRD, Aguiar RL, Alves TCA (2012) Fungal species that cause powdery mildew in greenhouse-grown cucumber and melon in Paraná State. Brazil Acta Scientiarum-Agronomy 34(3):247–252

    Google Scholar 

  • Ashrafi M, Azimi-Moqadam MR, Moradi P, MohseniFard E, Shekari F, Kompany-Zareh M (2018) Effect of drought stress on metabolite adjustments in drought tolerant and sensitive thyme. Plant Physiol Biochem 132:391–399

    Article  CAS  PubMed  Google Scholar 

  • Basu S, Ramegowda V, Kumar A, Pereira A (2016) Plant adaptation to drought stress. F1000Research. https://doi.org/10.12688/f1000research.7678.1

    Article  PubMed  PubMed Central  Google Scholar 

  • Bennett JA, Klironomos J (2019) Mechanisms of plant-soil feedback: interactions among biotic and abiotic drivers. New Phytol 222(1):91–96

    Article  PubMed  Google Scholar 

  • Bennett AJ, Bending GD, Chandler D, Hilton S, Mills P (2012) Meeting the demand for crop production: the challenge of yield decline in crops grown in short rotations. Biol Rev 87(1):52–71

    Article  PubMed  Google Scholar 

  • Bu R, Xiao X, Liao W, Hu Y, Li J, Lv J, Wang R, Xie J (2018) Exogenous Si alleviation of autotoxicity in Cucumber (Cucumis sativus L.) seed germination is correlated with changes in carbohydrate metabolism. Journal of Plant Growth Regulation 37(3):784–793

    Article  CAS  Google Scholar 

  • Cartenì F, Bonanomi G, Giannino F, Incerti G, Vincenot CE, Chiusano ML, Mazzoleni S (2016) Self-DNA inhibitory effects: underlying mechanisms and ecological implications. Plant Signal Behav 11(4):e1158381

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Cheng F, Cheng Z (2015) Research progress on the use of plant allelopathy in agriculture and the physiological and ecological mechanisms of allelopathy. Front Plant Sci 6:1020

    PubMed  PubMed Central  Google Scholar 

  • Christen D, Schönmann S, Jermini M, Strasser RJ, Défago G (2007) Characterization and early detection of grapevine (Vitis vinifera) stress responses to esca disease by in situ chlorophyll fluorescence and comparison with drought stress. Environ Exp Bot 60(3):504–514

    Article  CAS  Google Scholar 

  • Faseela P, Sinisha AK, Brestič M, Puthur JT (2020) Chlorophyll a fluorescence parameters as indicators of a particular abiotic stress in rice. Photosynthetica 58:293–300

    Article  CAS  Google Scholar 

  • Friedman J (2017) Allelopathy, autotoxicity, and germination. In: Kigel J (ed) Seed development and germination (eBook). Routledge, New York, pp 629–644

    Chapter  Google Scholar 

  • Hajihashemi S, Brestic M, Kalaji HM, Skalicky M, Noedoost F (2020) Environmental pollution is reflected in the activity of the photosynthetic apparatus. Photosynthetica 58:344–354

    Article  CAS  Google Scholar 

  • Henmi T, Miyao M, Yamamoto Y (2004) Release and reactive-oxygen-mediated damage of the oxygen-evolving complex subunits of PSII during photoinhibition. Plant Cell Physiol 45(2):243–250

    Article  CAS  PubMed  Google Scholar 

  • Hu W, Zhang Y, Huang B, Teng Y (2017) Soil environmental quality in greenhouse vegetable production systems in eastern China: current status and management strategies. Chemosphere 170:183–195

    Article  CAS  PubMed  Google Scholar 

  • Huang Y, Li W, Zhao L, Shen T, Sun J, Chen H, Kong Q, Nawaz MA, Bie Z (2017) Melon fruit sugar and amino acid contents are affected by fruit setting method under protected cultivation. Sci Hortic 214:288–294

    Article  CAS  Google Scholar 

  • Jiang HX, Chen LS, Zheng JG, Han S, Tang N, Smith BR (2008) Aluminum-induced effects on Photosystem II photochemistry in Citrus leaves assessed by the chlorophyll a fluorescence transient. Tree Physiol 28(12):1863–1871

    Article  CAS  PubMed  Google Scholar 

  • Jiao XL, Zhang XS, Lu XH, Qin R, Bi YM, Gao WW (2019) Effects of maize rotation on the physicochemical properties and microbial communities of American ginseng cultivated soil. Sci Rep 9(1):8615

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Kalaji HM, Schansker G, Ladle RJ, Goltsev V, Bosa K, Allakhverdiev SI, Elsheery NI (2014) Frequently asked questions about in vivo chlorophyll fluorescence: practical issues. Photosynth Res 122(2):121–158

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kapoor D, Tiwari A, Sehgal A, Landi M, Brestic M, Sharma A (2019) Exploiting the allelopathic potential of aqueous leaf extracts of Artemisia absinthium and Psidium guajava against Parthenium hysterophorus, a Widespread Weed in India. Plants 8(12):552

    Article  CAS  PubMed Central  Google Scholar 

  • Kato-Noguchi H, Nakamura K, Okuda N (2018) Involvement of an autotoxic compound in asparagus decline. J Plant Physiol 224:49–55

    Article  PubMed  CAS  Google Scholar 

  • Li Y, Li H, Li Y, Zhang S (2017) Improving water-use efficiency by decreasing stomatal conductance and transpiration rate to maintain higher ear photosynthetic rate in drought-resistant wheat. The Crop Journal 5(3):231–239

    Article  Google Scholar 

  • Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 11(5):591–592

    Article  CAS  Google Scholar 

  • Möhler H, Diekötter T, Herrmann JD, Donath TW (2018) Allelopathic vs. autotoxic potential of a grassland weed-evidence from a seed germination experiment. Plant Ecology and Diversity 11(4):539–549

    Article  Google Scholar 

  • Morshedloo MR, Craker LE, Salami A, Nazeri V, Sang H, Maggi F (2017) Effect of prolonged water stress on essential oil content, compositions and gene expression patterns of mono-and sesquiterpene synthesis in two oregano (Origanum vulgare L.) subspecies. Plant Physiol Biochem 111:119–128

    Article  CAS  PubMed  Google Scholar 

  • Moshelion M, Halperin O, Wallach R, Oren RAM, Way DA (2015) Role of aquaporins in determining transpiration and photosynthesis in water-stressed plants: crop water-use efficiency, growth and yield. Plant, Cell Environ 38(9):1785–1793

    Article  CAS  Google Scholar 

  • Nouri MZ, Moumeni A, Komatsu S (2015) Abiotic stresses: insight into gene regulation and protein expression in photosynthetic pathways of plants. Int J Mol Sci 16(9):20392–20416

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nowicka B, Ciura J, Szymańska R, Kruk J (2018) Improving photosynthesis, plant productivity and abiotic stress tolerance–current trends and future perspectives. J Plant Physiol 231:415–433

    Article  CAS  PubMed  Google Scholar 

  • Parihar P, Singh S, Singh R, Singh VP, Prasad SM (2015) Effect of salinity stress on plants and its tolerance strategies: a review. Environ Sci Pollut Res 22(6):4056–4075

    Article  CAS  Google Scholar 

  • Pontius J, Hallett R (2014) Comprehensive methods for earlier detection and monitoring of forest decline. Forest Science 60(6):1156–1163

    Article  Google Scholar 

  • Rapacz M, Wójcik-Jagła M, Fiust A, Kalaji HM, Koscielniak J (2019) Genome-wide associations of chlorophyll fluorescence OJIP transient parameters connected with soil drought response in barley. Front Plant Sci 10:78

    Article  PubMed  PubMed Central  Google Scholar 

  • Ripoll J, Bertin N, Bidel LP, Urban L (2016) A user’s view of the parameters derived from the induction curves of maximal chlorophyll a fluorescence: perspectives for analyzing stress. Front Plant Sci 7:1679

    Article  PubMed  PubMed Central  Google Scholar 

  • Silva MA, Albuquerque TG, Alves RC, Oliveira MBP, Costa HS (2018) Melon (Cucumis melo L) by-products: Potential food ingredients for novel functional foods? Trends in Food Science & Technology 98:181–189

    Article  CAS  Google Scholar 

  • Strasser RJ, Tsimilli-Michael M, Srivastava A (2004) Analysis of the chlorophyll a fluorescence transient. In: Papageorgiou GC (ed) Chlorophyll a fluorescence(eBook). Springer, Dordrecht, pp 321–362

    Chapter  Google Scholar 

  • Sun ZK, He WM (2019) Autotoxicity of root exudates varies with species identity and soil phosphorus. Ecotoxicology 28(4):429–434

    Article  CAS  PubMed  Google Scholar 

  • Sunil B, Saini D, Bapatla RB, Aswani V, Raghavendra AS (2019) Photorespiration is complemented by cyclic electron flow and the alternative oxidase pathway to optimize photosynthesis and protect against abiotic stress. Photosynth Res 139(1–3):67–79

    Article  CAS  PubMed  Google Scholar 

  • Talukder MR, Asaduzzaman M, Tanaka H, Asao T (2019) Application of alternating current electro-degradation improves retarded growth and quality in lettuce under autotoxicity in successive cultivation. Sci Hortic 252:324–331

    Article  Google Scholar 

  • Van Heerden PD, Tsimilli-Michael M, Krüger GH, Strasser RJ (2003) Dark chilling effects on soybean genotypes during vegetative development: parallel studies of CO2 assimilation, chlorophyll a fluorescence kinetics OJIP and nitrogen fixation. Physiol Plant 117(4):476–491

    Article  PubMed  Google Scholar 

  • Xia ZC, Kong CH, Chen LC, Wang P, Wang SL (2016) A broadleaf species enhances an autotoxic conifers growth through belowground chemical interactions. Ecology 97(9):2283–2292

    Article  PubMed  Google Scholar 

  • Xin A, Li X, Jin H, Yang X, Zhao R, Liu J, Qin B (2019) The accumulation of reactive oxygen species in root tips caused by autotoxic allelochemicals–A significant factor for replant problem of Angelica sinensis (Oliv.) Diels. Industrial Crops and Products 138:111432

    Article  CAS  Google Scholar 

  • Yang RX, Gao ZG, Liu X, Yao Y, Cheng Y (2014) Root exudates from muskmelon (Cucumis melon. L) induce autotoxicity and promote growth of Fusarium oxysporum f. sp. melonis. Allelopathy Journal 33(2):175–188

    Google Scholar 

  • Zhang H, Sonnewald U (2017) Differences and commonalities of plant responses to single and combined stresses. Plant J 90(5):839–855

    Article  CAS  PubMed  Google Scholar 

  • Zhang H, Xu N, Li X, Long J, Sui X, Wu Y, Sun GY (2018) Arbuscular mycorrhizal fungi (Glomus mosseae) improves growth, photosynthesis and protects photosystem II in leaves of Lolium perenne L. in cadmium contaminated soil. Frontiers in plant science 9:1156

    Article  PubMed  PubMed Central  Google Scholar 

  • Zhang Z, Fan J, Wu J, Zhang L, Wang J, Zhang B, Wang-Pruski G (2020) Alleviating effect of silicon on melon seed germination under autotoxicity stress. Ecotoxicol Environ Saf 188:109901

    Article  CAS  PubMed  Google Scholar 

  • Zhao G, Lian Q, Zhang Z, Fu Q, He Y, Ma S, Wang H (2019) A comprehensive genome variation map of melon identifies multiple domestication events and loci influencing agronomic traits. Nat Genet 51(11):1607–1615

    Article  CAS  PubMed  Google Scholar 

  • Zhu S, Wang Y, Xu X, Liu T, Wu D, Zheng X, Tang S, Dai Q (2018) Potential use of high-throughput sequencing of soil microbial communities for estimating the adverse effects of continuous cropping on ramie (Boehmeria nivea L. Gaud). PloS one 13(5):e0197095

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Zushi K, Matsuzoe N (2017) Using of chlorophyll a fluorescence OJIP transients for sensing salt stress in the leaves and fruits of tomato. Sci Hortic 219:216–221

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by the Special Innovation Foundation (CXZX2016108, CXZX2017168) from Fujian Agriculture and Forestry University (FAFU) and MinJiang Scholar fund from Fujian Province and FAFU (116-114120019).

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Authors and Affiliations

  1. Joint FAFU-Dalhousie Lab, College of Horticulture, Fujian Agriculture and Forestry University, No.15 Shangxiadian Road, Cangshan District, Fuzhou, 350002, Fujian Province, China

    Zhizhong Zhang, Jinghua Wu, Yupei Xi & Lizhen Zhang

  2. Fujian Feisi Agricultural Science and Technology Development Limited Company, Fuzhou, 350002, China

    Qiang Gao

  3. Faculty of Agriculture, Department of Plant, Food, and Environmental Sciences, Dalhousie University, Truro, NS, B2N 5E3, Canada

    Gefu Wang-Pruski

Authors
  1. Zhizhong Zhang
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  2. Jinghua Wu
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  3. Yupei Xi
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  4. Lizhen Zhang
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  5. Qiang Gao
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  6. Gefu Wang-Pruski
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Contributions

ZZ Zhang and JH Wu designed the experiments, analyzed the data and wrote the manuscript. ZZ Zhang, JH Wu and Q Gao performed the experiments. YP Xi and LZ Zhang contributed reagents and materials. JH Wu and G Wang-Pruski revised the manuscript. JH Wu and ZZ Zhang provided financial support and final approval of manuscript.

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Correspondence to Zhizhong Zhang.

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Zhang, Z., Wu, J., Xi, Y. et al. Effects of Autotoxicity on Seed Germination, Gas Exchange Attributes and Chlorophyll Fluorescence in Melon Seedlings. J Plant Growth Regul 41, 993–1003 (2022). https://doi.org/10.1007/s00344-021-10355-w

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  • DOI: https://doi.org/10.1007/s00344-021-10355-w

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