Skip to main content


Log in

Cladosporium cladosporioides C24G Modulates Gene Expression and Enzymatic Activity During Leaf Blast Suppression in Rice Plants

  • Published:
Journal of Plant Growth Regulation Aims and scope Submit manuscript


The inclusion of biological control in the integrated management of rice blast (Magnaporthe oryzae) is an alternative to reduce pesticides application. C24G, classified, as Cladosporium cladosporioides was isolated from the phylloplane of the rice plant, therefore, adapted to natural conditions of the original habitat. This study aimed to compare four application methods of C24G in rice plants to suppress leaf blast together with the increase in enzymatic activity and expression of defense genes. It was conducted by four assays (1: seed and soil, 2: soil drenching, 3: foliar spray pulverization—preventive and 4: foliar spray pulverization—curative) for choosing the best application method. The best methods identified were further investigated for the activity of Chitinase (CHI), β-1,3-Glucanase (GLU), Lipoxygenase (LOX), Phenylalanine ammonia-lyase (PAL), and Peroxidase (POX) and the expression of Gns1, JIOsPR10, LOX-RLL, and PR1b genes by Real-time PCR. The preventive foliar spray pulverization suppressed up to 83.78% of leaf blast severity, increasing enzymes (CHI, GLU, LOX, and PAL) activity and genes (JIOsPR10, LOX-RLL, and PR1b) expression. We conclude that Cladosporium cladosporioides isolated C24G is a potential biological agent. To prove its potential as a component of sustainable blast management, it should be tested under field conditions. The application of C24G isolate in rice fields can reduce the number of fungicides spraying, generating greater rentability and decreasing environmental contaminations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others


Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Marta Cristina Corsi de Filippi.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chaibub, A.A., de Sousa, T.P., de Araújo, L.G. et al. Cladosporium cladosporioides C24G Modulates Gene Expression and Enzymatic Activity During Leaf Blast Suppression in Rice Plants. J Plant Growth Regul 39, 1140–1152 (2020).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: