Action of N-Succinyl and N,O-Dicarboxymethyl Chitosan Derivatives on Chlorophyll Photosynthesis and Fluorescence in Drought-Sensitive Maize
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Chitosan induces plant tolerance to various abiotic stresses, including water deficit. However, its use may be limited, due to its constitution and low solubility in water. Thus, chemical modifications were proposed in this study with the objective of potentializing its biological effects in maize plants. The derivatives were semi-synthesized (N-succinyl and N,O-dicarboxymethyl) and, together with chitosan, they were applied, via the leaf, in a drought-sensitive maize hybrid (BRS1030) under pre-flowering water deficit. The water deficit was maintained for 15 days and the analyses were performed at the beginning and end of stress, and also in rehydration. Leaf water potential, gas exchange, chlorophyll fluorescence, and content of chloroplastidic pigments were evaluated. The use of the derivatives modulated photosynthesis parameters, affecting the involved mechanisms, such as stomatal activity, water use efficiency and photosystem II activity. Chlorophyll fluorescence indicated that the antenna complex was damaged by the water deficit condition, with a decrease in the energy flux in the electron transport chain and in the photochemical phase of photosynthesis. However, the spraying of chitosan derivatives induced tolerance to water deficit, suggesting that chitosan derivatives are more bioavailable to plants. Water stress decreases pigment content, but both the application of chitosan and derivatives increased these contents. It is concluded that chitosan derivatives improved the photosynthetic parameters in maize susceptible to drought, inducing tolerance to this stress, and the possible reasons and consequences are discussed.
KeywordsWater deficit Zea mays L. Gas exchange Biostimulant Quenching Leaf water potential
The authors would like to thank Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), for the master’s degree scholarship (C. O. Reis) and project financing (APQ-00651-14).
This study was funded by Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) and Conselho Nacional de Desenvolvimento Científico e Tecnológico. Project financing: APQ-00651-14.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
Research Involving Human and Animal Participants
This article does not contain any studies with human participants (or animals) performed by any of the authors.
- Boonlertnirun S, Sarobol ED, Meechoui S, Sooksathan I (2007) Drought recovery and grain yield potential of rice after chitosan application. Kasetsart J Nat Sci 41:1–6Google Scholar
- FAO (2002) Deficit irrigation practices. Water reports, n. 22. RomeGoogle Scholar
- Genty B, Harbinson J, Cailly AL, Rizza F (1996) Fate of excitation at PS II in leaves: the non- photochemical side. Presented at: The Third BBSRC Robert Hill Symposium on Photosynthesis, March 31 to April 3, University of Sheffield, Department of Molecular Biology and Biotechnology, Western Bank, Sheffield, UK, pp 28Google Scholar
- Ghahfarokhi MG, Mansurifar S, Taghizadeh-Mehrjardi R, Saeidi M, Jamshidi AM, Ghasemi E (2015) Effects of drought stress and rewatering on antioxidant systems and relative water content in different growth stages of maize (Zea mays L.) hybrids. Arch Agron Soil Sci 61(4):493–506. https://doi.org/10.1080/03650340.2014.943198 CrossRefGoogle Scholar
- Ibrahim EA, Ramadan WA (2015) Effect of zinc foliar spray alone and combined with humic acid or/and chitosan on growth, nutrient elements content and yield of dry bean (Phaseolus vulgaris L.) plants sown at different dates. Sci Hort 184: 101–105. https://doi.org/10.1016/j.scienta.2014.11.010 CrossRefGoogle Scholar
- Kalaji MH, Guo P (2008) Chlorophyll fluorescence: a useful tool in barley plant breeding programs. In: Sanchez A, Gutierrez SJ (eds) Photochemistry research progress. Nova Publishers, New York, pp 439–463Google Scholar
- Karimi LN, Khanahmadi M, Moradi B (2012) Accumulation and phytotoxicity of lead in Cynara scolymus. Indian J Sci Technol 5:3634–3641Google Scholar
- Limpanavech P, Chaiyasuta S, Vongpromek R, Pichyangkura R, Khunwasi C, Chadchawan S, Lotrakul P, Bunjongrat R, Chaidee A, Bangyeekhun T (2008) Chitosan effects on floral production, gene expression, and anatomical changes in the Dendrobium orchid. Sci Hortic 116: 65–72. https://doi.org/10.1016/j.scienta.2007.10.034 CrossRefGoogle Scholar
- Lizarraga-Pauli EG, Torres-Pacheco I, Moreno-Martinez E, Miranda-Castro SP (2011) Chitosan application in maize (Zea mays) to counteract the effects of abiotic stress at seedling level. Afr J Biotechnol 10(34):6439–6446Google Scholar
- Martins M, Carvalho M, Carvalho DT, Barbosa S, Doriguetto AC, Magalhaes PC, Ribeiro C (2018) Physicochemical characterization of chitosan and its effects on early growth, cell cycle and root anatomy of transgenic and non-transgenic maize hybrids. Aust J Crop Sci 12:56. https://doi.org/10.21475/ajcs.18.12.01.pne649 CrossRefGoogle Scholar
- Mondal MMA, Malek MA, Puteh AB, Ismail MR, Ashrafuzzaman M, Naher L (2012) Effect of foliar application of chitosan on growth and yield in okra. Aust J Crop Sci 6:918–921Google Scholar
- Mondal MMA, Puteh AB, Dafader NC, Rafii MY, Malek MA (2013) Foliar application of chitosan improves growth and yield in maize. J Food Agric Environ 11:520–523Google Scholar
- Peltier G, Cournac L (2002) Chlororespiration. Annu Rev Plant Biol 53:523–550. https://doi.org/10.1146/annurev.arplant.53.100301.135242 CrossRefGoogle Scholar
- Perdomo JA, Capó-Bauçà S, Carmo-Silva E, Galmés J (2017) Rubisco and rubisco activase play an important role in the biochemical limitations of photosynthesis in rice, wheat, and maize under high temperature and water deficit. Front Plant Sci 8:490. https://doi.org/10.3389/fpls.2017.00490 CrossRefGoogle Scholar
- Pirbalouti AG, Malekpoor F, Salimi A, Golparvar A (2017) Exogenous application of chitosan on biochemical and physiological characteristics, phenolic content and antioxidant activity of two species of basil (Ocimum ciliatum and Ocimum basilicum) under reduced irrigation. Sci Hortic 217:114–122. https://doi.org/10.1016/j.scienta.2017.01.031 CrossRefGoogle Scholar
- Ramírez M, Rodriguez AT, Alfonso L, Peniche C (2010) Chitin and its derivatives as biopolymers with potential agricultural applications. Biotechnol Appl 27:270–276Google Scholar
- Rinderle U, Lichtenthaler KK (eds) (1988) Applications of chlorophyll fluorescence. Kluwer Academic Publishers, DordrechtGoogle Scholar
- Souza TC, Castro EM, Magalhaes PC, Lino LO, Alves ET, Albuquerque PEP (2013a) Morphophysiology, morphoanatomy, and grain yield under field conditions for two maize hybrids with contrasting response to drought stress. Acta Physiol Plant 35:3201–3321. https://doi.org/10.1007/s11738-013-1355-1 CrossRefGoogle Scholar
- Souza TC, Castro EM, Magalhães PC, Castro EM, Albuquerque PEP, Marabesi MA (2013b) The influence of ABA on water relation, photosynthesis parameters, and chlorophyll fluorescence under drought conditions in two maize hybrids with contrasting drought resistance. Acta Physiol Plant 35:515–527. https://doi.org/10.1007/s11738-012-1093-9 CrossRefGoogle Scholar
- Souza TC, Magalhães PC, Castro EM, Carneiro NP, Padilha FA, Gomes Júnior CC (2014) ABA application to maize hybrids contrasting for drought tolerance: changes in water parameters and in antioxidant enzyme activity. Plant Growth Regul 73:205–2017. https://doi.org/10.1007/s10725-013-9881-9 CrossRefGoogle Scholar
- Wang X, Vigjevic M, Liu F, Jacobsen S, Jiang D, Wollenweber B (2015) Drought priming at vegetative growth stages improves tolerance to drought and heat stresses during grain fi lling in spring wheat (Triticum aestivum L. cv. Vinjett). Plant Growth Regul 75:677–687. https://doi.org/10.1007/s10725-014-9969-x CrossRefGoogle Scholar