Journal of Plant Growth Regulation

, Volume 38, Issue 2, pp 600–605 | Cite as

l-Arginine Pretreatment Enhances Drought Resistance of Sunflower (Helianthus annuus L.) Plants by Increase in Polyamines Content

  • Atiat Mohamed HassanEmail author
  • Hala Ezzat Mohamed


A significant reduction of fresh, dry biomass, shoot height, root length, succulent value, and leaf area were noticed as well as a significant accumulation of H2O2 and MDA contents in shoots and roots of sunflower plants in response to drought stress. Pretreatment of sunflower seeds with 2 mM arginine (Arg) resulted in a significant increase in all growth parameters and reduced H2O2 and MDA contents in drought-stressed plants. This was accompanied with a significant increase of total polyamine contents (PAs) in shoots and roots. The results of this study indicate that Arg-pretreatment and the increase of PAs especially in roots might prevent—to some extent—the oxidative stress of H2O2 on plasma membranes and hence maintain the water status and growth of drought-stressed sunflower plants and reveal the role of polyamines as a strategy mechanism against drought stress.


Helianthus annuus Drought stress Arginine Putrescine Spermidine Spermine 



We would like to express our profound gratefulness to Prof. Dr. Nabil El-Sayed Saber, for his inspiring guidance and his kind constructive advice.


This study was funded by Faculty of Science-Alexandria University.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Atifriaz Z, Younis A, Riaztaj A, Karim A, Tara U, Riaz S (2013) Effect of drought stress on growth and flowering of marigold (Tagetes erecta L.). Pak J Bot 45:1231–1231Google Scholar
  2. Bonales-Alatorre E, Shabala S, Chen ZH, Pottosin I (2013) Reduced tonoplast fast-activating and slow-activating channel activity is essential for conferring salinity tolerance in a facultative halophyte, Quinoa. Plant Physiol 162:940–952CrossRefGoogle Scholar
  3. Devi R, Kaur N, Gupta AK (2011) Potential of antioxidant enzymes in depicting drought tolerance of wheat (Triticuam aestivum. L). Indian J Biochem Biophys 11:2567–2572Google Scholar
  4. Esmer I, Tuney I, Ozakca DU, Sukatar A (2017) Protective effects of polyamines against UV-A and UV-B illumination in Physcia semipinnata thalli. Bot Serbica 41:17–24Google Scholar
  5. Gill S, Tuteja N (2010a) Polyamines and abiotic stress tolerance in plants. Plant Signal Behav J 5:26–33Google Scholar
  6. Gill SS, Tuteja N (2010b) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930CrossRefGoogle Scholar
  7. Guo Z, Tan J, Zhuo C, Wang C, Xiang B, Wang Z (2014) Abscisic acid, H2O2 and nitric oxide interactions mediated cold-induced S-adenosyl methionine synthetase in Medicago sativa subsp. falcata that confers cold tolerance through up-regulating polyamine oxidation. Plant Biotechnol J 12:601–612CrossRefGoogle Scholar
  8. Gupta K, Dey A, Gupta B (2013) Plant polyamines in abiotic stress responses. Acta Physiol Plant 35:2015–2036CrossRefGoogle Scholar
  9. Hanfrey C, Sommer S, Mayer MJ, Burtin D, Michael AJ (2001) Arabidopsis polyamine biosynthesis: absence of ornithine decarboxylase and the mechanism of arginine decarboxylase activity. Plant J 27:551–560CrossRefGoogle Scholar
  10. Kakkar RK, Sawhney VK (2002) Polyamine research in plants—a changing perspective. Physiol Plant 116:281–292CrossRefGoogle Scholar
  11. Kakkar RK, Bhaduri S, Rai VK, Kumar S (2000) Amelioration of NaCl stress by arginine in rice seedlings: changes in endogenous polyamines. Biol Plant 43:4122–4194Google Scholar
  12. Kaur-Sawhney R, Tiburcio AF, Altabella T, Galston AW (2003) Polyamines in plants: an overview. J Cell Mol Biol 2:11–12Google Scholar
  13. Kavas M, Baloglu MC, Akca O, Kose FS, Gokcay D (2013) Effect of drought stress on oxidative damage and antioxidant enzyme activity in melon seedlings. Turk J Biol 37:491–498CrossRefGoogle Scholar
  14. Liu JH, Honda C, Moriguchi T (2006) Involvement of polyamine in floral and fruit development. JARQ 40:51–58CrossRefGoogle Scholar
  15. Luna CM, Pastori GM, Driscoll S, Groten K, Bernard S, Foyed CH (2005) Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat. J Exp Bot 56:4114–4120Google Scholar
  16. Manivannan P, Jaleel CA, Kishorekumal BA, Somasundaram R, Lakshmanan GM, Panneerselvam R (2007) Growth, biochemical modification and proline metabolism in (Helianthus annus L.) as induced by drought stress. Colloids Surf B 59:1411–1449CrossRefGoogle Scholar
  17. Molassiotis A, Fotopoulos V (2011) Oxidative and nitrosative signaling in plants: two branches in the same tree? Plant Signal Behav 6:2102–2114CrossRefGoogle Scholar
  18. Moschou PN, Wu J, Cona A, Tavladoraki P, Angelini R, Roubelakis-Angelakis KA (2012) The polyamines and their catabolic products are significant players in the turnover of nitrogenous molecules in plants. J Exp Bot 63:5003–5015CrossRefGoogle Scholar
  19. Nahar k, Hasanuzzaman M. Alam MM, Rahman A, Mahmud JA, Suzuki T, Fujita M (2017) Insights into spermine-induced combined high temperature and drought tolerance in mung bean: osmo-regulation and roles of antioxidant and glyoxalase system. Protoplasma 254:445–460CrossRefGoogle Scholar
  20. Nasibi F, Yaghoobi M, Kalantari KH (2011) Effect of exogenous arginine on alleviation of oxidative damage in tomato plant under water stress. J Plant Interact 6:2912–2996CrossRefGoogle Scholar
  21. Nasibi F, Kalantari KM, Barand A (2014) Effects of seed pre-treatment with L-arginine on improvement of seedling growth and alleviation of oxidative damage in canola plants subjected to salt stress. Iran J Plant Physiol 5:12171–12224Google Scholar
  22. Peng D, Wang X, Li Z, Zhang Y, Peng Y, Li Y, He X, Zhang X, Ma X, Huang L (2016) NO is involved in spermidine-induced drought tolerance in white clover via activation of antioxidant enzymes and genes. Protoplasma 253:12431–12454CrossRefGoogle Scholar
  23. Pottosin I, Shabala S (2014) Polyamines control of cation transport across plant membranes: implications for ion homeostasis and abiotic stress signaling. Front plant sci 5:154–170CrossRefGoogle Scholar
  24. Sequera-Mutiozaball M, Antoniou C, Tiburcio AF, Alcázar R, Fotopoulos V (2017) Polyamines: emerging hubs promoting drought and salt Stress tolerance in plants. Curr Mol Bio Rep 3:283–286CrossRefGoogle Scholar
  25. Souza CC, Oliveira FA, Silva IF, Amorim Neto MS (2000) Evaluation of methods of available water determinaton and irrigation management in “terra roxa” under cotton crop. Rev Bras Eng Agric Ambiental 4:338–342CrossRefGoogle Scholar
  26. Takahashi T, Kakehi J (2010) Polyamines: ubiquitous polycations with unique roles in growth and stress responses. Ann Bot 105:1–6CrossRefGoogle Scholar
  27. Velikova V, Yordanov I, Edereva A (2000) Oxidative stress and some antioxidant systems in acid rain treated bean plants. Protective role of exogenous polyamines. Plant Sci 151:59–66CrossRefGoogle Scholar
  28. Wentworth M, Murchie MH, Gray JE, Villegas D, Pastenes C, Pinto M, Horton P (2006) Differential adaptation of two varieties of common bean to abiotic stress. II. Acclimation of photosynthesis. J Exp Bot 57:699–709CrossRefGoogle Scholar
  29. Yang F, Miao LF (2010) Adaptive responses to progressive drought stress in two poplar species originating from different altitudes. Silva Fennica 44:23–37Google Scholar
  30. Yang J, Zhang J, Liu K, Wang Z, Liu L (2007) Involvement of polyamines in the drought resistance of rice. J Exp Bot 58:1545–1555Google Scholar
  31. Zeid IM (2009) Effect of arginine and urea on polyamines content and growth of bean under salinity stress. Acta Physiol Plant 31:65–70CrossRefGoogle Scholar
  32. Zhange L, Ackley AR, Pilon-Smits EAH (2007) Variation in selenium tolerance and accumulation among 19 Arabidopsis thaliana accessions. J Plant Physiol 164:327–336Google Scholar
  33. Zhao H, Yang H (2008) Exogenous polyamines alleviate the lipid peroxidation induced by cadmium chloride stress in Malus hupehensis Rehd. Sci Hortic J 116:442–447Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Botany and Microbiology Department, Faculty of ScienceAlexandria UniversityAlexandriaEgypt

Personalised recommendations