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Adventitious Shoot Induction from Internode and Root Explants in a Semiparasitic Herb Monochasma savatieri Franch ex Maxim

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Abstract

A highly efficient protocol for the induction of adventitious shoots from young internode and root explants of a semiparasitic medicinal herb Monochasma savatieri Franch ex Maxim was developed. MS basal medium supplemented with 5 µM thidiazuron (TDZ) induced 32 adventitious shoots/explant, which was double the number obtained using the same concentration of 6-benzyladenine (BA). Hyperhydric shoots were observed when 10 µM of any cytokinin was added to MS media. Use of any cytokinin at 2.5 µM produced an average of 14–21 adventitious shoots/root explant. Shoots formed roots in vitro more effectively than α-naphthaleneacetic acid when indole-3-butyric acid and indole-3-acetic acid were used at 1.0 µM. Two-month-old rooted plantlets were transplanted to vermiculite and 70% survived after 4 months.

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References

  • Bhat SR, Chitralekha P, Chandel KPS (1992) Regeneration of plants from long term root culture of lime, Citrus aurantifolia (Christm.) Swing. Plant Cell Tiss Organ Cult 29:19–25

    Article  Google Scholar 

  • Deeks SJ, Shamoun SF, Punja ZK (1999) Tissue culture of parasitic flowering plants: methods and applications in agriculture and forestry. In Vitro Cell Dev Biol-Plant 35:369–381

    Article  Google Scholar 

  • Hong D, Yang H, Jin C, Holmgren NH (1998) Scrophulariaceae. In: Wu ZY, Raven PH (eds) Flora of China, vol 18. Science Press and Missouri Botanical Garden Press, Beijing, p 211–212

    Google Scholar 

  • Jitendra GP, Mahendra LA, Kirti MK, Sayantan P, Vijay PB, Polavarapu BKK, Tukaram DN (2013) In vitro propagation and production of cardiotonic glycosides in shoot cultures of Digitalis purpurea L. by elicitation and precursor feeding. Appl Microbiol Biotechnol 97:2379–2393

    Article  Google Scholar 

  • Karim MZ, Yokota S, Rahman MM, Eizawa J, Saito Y, Azad MAK, Ishiguri F, Iizuka K, Yoshizawa N (2007) Efficient adventitious shoot regeneration from root explants of Aralia elata Seem. Int J Bot 3:390–393

    Article  CAS  Google Scholar 

  • Kelkar SM, Krishnamurthy KV (1998) Adventitious shoot regeneration from root, internode, petiole and leaf explants of Piper colubrinum Link. Plant Cell Rep 17:721–725

    Article  CAS  Google Scholar 

  • Knoll KA, Short KC, Curtis IS, Power JB, Davey MR (1997) Shoot regeneration from cultured root explants of spinach (Spinacia oleracea L.): a system for Agrobacterium transformation. Plant Cell Rep 17:96–101

    Article  CAS  Google Scholar 

  • Lü JF, Teixeira da Silva JA, Ma GH (2013) Plant regeneration via somatic embryogenesis and shoot organogenesis from immature cotyledons of Camellia nitidissima. J Plant Physiol 170:1202–1211

    Article  PubMed  Google Scholar 

  • Martínez-Bonfil BP, Salcedo-Morales G, López-Laredo AR, Ventura-Zapata E, Evangelista-Lozano S, Trejo-Tapia G (2011) Shoot regeneration and determination of iridoid levels in the medicinal plant Castilleja tenuiflora Benth. Plant Cell Tiss Organ Cult 107:195–203

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Olmstead RG, dePamphilis CW, Wolfe AD, Young ND, Elisons WJ, Reeves PA (2001) Disintegration of the Scrophulariaceae. Am J Bot 88:348–361

    Article  CAS  PubMed  Google Scholar 

  • Rybczynski JJ, Dabrowski L, Badzian T (1995) Changes in morphological potential of shoot regeneration from root explants of Lotus corniculatus L. seedling. Acta Physiol Plant 17:211–218

    Google Scholar 

  • Shi MF, He WJ, Liu YL, Li XR, Yang SL, Xu QM (2013) Protective effect of total phenylethanoid glycosides from Monochasma savatieri Franch on myocardial ischemia injury. Phytomedicine 20:1251–1255

    Article  CAS  PubMed  Google Scholar 

  • Sim GE, Goh CJ, Loh CS (1989) Micropropagation of Citrus mitis Blanco: multiple bud formation from shoot and root explants in the presence of 6-benzylaminopurine. Plant Sci 59:203–210

    Article  CAS  Google Scholar 

  • Tomilov A, Tomilova N, Yoder JI (2004) In vitro haustorium development in roots and root cultures of the hemiparasitic plant Triphysaria versicolor. Plant Cell Tiss Organ Cult 77:257–265

    Article  Google Scholar 

  • Vinocur B, Carmi T, Altman A, Ziv M (2000) Enhanced bud regeneration in aspen (Populus tremula L.) roots cultured in liquid media. Plant Cell Rep 19:1146–1154

    Article  CAS  Google Scholar 

  • Wolf SJ, Timko MP (1991) In vitro root culture a novel approach to study the obligate parasite Striga asiatica L. Kuntze. Plant Sci 73:233–242

    Article  CAS  Google Scholar 

  • Yang YZ (2009) Plantlet regeneration of Monochasma savatieri Franch. ex Maxim. in vitro culture from inducing adventitious root. Plant Physiol Commun 7:693–694 (in Chinese)

    Google Scholar 

  • Zhang MH, Chen YL, Ouyang Y, Huang ZG, Teixeira da Silva JA, Ma GH (2015) The biology and haustorial anatomy of semi-parasitic Monochasma savatieri Franch. ex Maxim. Plant Growth Reg 75:473–481

    Article  CAS  Google Scholar 

  • Zheng W, Tan XQ, Guo LJ, Kong FF, Lu P, Ni DJ, Wang P (2012) Chemical constituents from Monochasma savatieri. Chin J Nat Med 10:102–104

    Article  CAS  Google Scholar 

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Authors Contribution

Conceived and designed the experiments: Xinhua Zhang, Jaime A. Teixeira da Silva, Guohua Ma. Performed the experiments: Yueya Zhang, Yulu Chen. Wrote the paper, analyzed the data and edited all versions: Yueya Zhang, Yulu Chen, Xinhua Zhang, Jaime A. Teixeira da Silva, Guohua Ma.

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Correspondence to Xinhua Zhang, Jaime A. Teixeira da Silva or Guohua Ma.

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The authors declare no conflicts of interest.

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Yueya Zhang and Yulu Chen have contributed equally.

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Zhang, Y., Chen, Y., Zhang, X. et al. Adventitious Shoot Induction from Internode and Root Explants in a Semiparasitic Herb Monochasma savatieri Franch ex Maxim. J Plant Growth Regul 36, 799–804 (2017). https://doi.org/10.1007/s00344-017-9681-y

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  • DOI: https://doi.org/10.1007/s00344-017-9681-y

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