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UV-B-induced Inhibition of Stem Elongation and Leaf Expansion in Pea Depends on Modulation of Gibberellin Metabolism and Intact Gibberellin Signalling

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Abstract

Information on the involvement of elongation-controlling hormones, particularly gibberellin (GA), in UV-B modulation of stem elongation and leaf growth, is limited. We aimed to study the effect of UV-B on levels of GA and indole-3-acetic acid (IAA) as well as involvement of GA in UV-B inhibition of stem elongation and leaf expansion in pea. Reduced shoot elongation (13%) and leaf area (37%) in pea in response to a 6-h daily UV-B (0.45 W m−2) exposure in the middle of the light period for 10 days were associated with decreased levels of the bioactive GA1 in apical stem tissue (59%) and young leaves (69%). UV-B also reduced the content of IAA in young leaves (35%). The importance of modulation of GA metabolism for inhibition of stem elongation in pea by UV-B was confirmed by the lack of effect of UV-B in the le GA biosynthesis mutant. No UV-B effect on stem elongation in the la cry-s (della) pea mutant demonstrates that intact GA signalling is required. In conclusion, UV-B inhibition of shoot elongation and leaf expansion in pea depends on UV-B modulation of GA metabolism in shoot apices and young leaves and GA signalling through DELLA proteins. UV-B also affects the IAA content in pea leaves.

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Acknowledgements

We gratefully acknowledge Dr. Irina Zaharia, Dr. Suzanne Abrams and technical staff at the Plant Biotechnology Institute of the National Research Council of Canada, Saskatoon, Canada for the work with the hormone profiling, Prof. John Ross for the pea mutants, Marit Siira and Ida K Hagen for excellent technical assistance, and the Norwegian Agency for Development Cooperation, the Norwegian Research Council (NFR 223268/F50; CERAD), the Norwegian University of Life Sciences and Hawassa University, Ethiopia, for funding.

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Authors and Affiliations

  1. Department of Plant Sciences, Norwegian University of Life Sciences, N1432, Ås, Norway

    Amsalu Gobena Roro, Suzanne A. F. Dukker, Tone I. Melby, Sissel Torre & Jorunn E. Olsen

  2. CERAD, Norwegian University of Life Sciences, N1432, Ås, Norway

    Amsalu Gobena Roro, Knut Asbjørn Solhaug, Sissel Torre & Jorunn E. Olsen

  3. Department of Ecology and Natural Resource Management, Norwegian University of Life Sciences, N1432, Ås, Norway

    Knut Asbjørn Solhaug

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  1. Amsalu Gobena Roro
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Correspondence to Jorunn E. Olsen.

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Roro, A.G., Dukker, S.A.F., Melby, T.I. et al. UV-B-induced Inhibition of Stem Elongation and Leaf Expansion in Pea Depends on Modulation of Gibberellin Metabolism and Intact Gibberellin Signalling. J Plant Growth Regul 36, 680–690 (2017). https://doi.org/10.1007/s00344-017-9671-0

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