Journal of Plant Growth Regulation

, Volume 35, Issue 3, pp 744–754 | Cite as

Comparative Proteomic Analysis Reveals Nitrogen Fertilizer Increases Spikelet Number per Panicle in Rice by Repressing Protein Degradation and 14-3-3 Proteins

  • Chengqiang Ding
  • Yan Wang
  • Zhongyuan Chang
  • Siliang You
  • Zhenghui Liu
  • Shaohua Wang
  • Yanfeng Ding
Article

Abstract

The spikelet number per panicle is established in the early stages of panicle development. Nitrogen fertilizer application before panicle initiation is known to increase spikelet number, which is one of the most important traits in rice productivity determination. However, the basic proteomic mechanism remains poorly understood. The present study shows that nitrogen fertilizer significantly increased spikelet number and grain yield in rice. Proteomic variations were further analyzed in young panicles at the secondary panicle branch initiation and spikelet meristem initiation under nitrogen fertilizer treatment. Proteomic analysis identified 63 proteins with significant differential accumulation in young panicles under nitrogen fertilizer treatment. Proteolysis represents the largest functional category, which suggests that protein degradation is an important pathway in the response to nitrogen fertilizer. Importantly, nitrogen fertilizer significantly reduced 14-3-3 proteins, which interact with key enzymes associated with carbon and nitrogen metabolism, and the rice FT homologue Hd3a. Real-time PCR revealed that Hd3a signaling is also repressed by nitrogen fertilizer in leaves. This study contributes to a better understanding of the regulation of nitrogen fertilizers in the flowering pathway leading to panicle development. The identification of novel genes provides new insight into the profound impacts of nitrogen fertilizer on panicle development in rice.

Keywords

14-3-3 proteins, nitrogen fertilizer Rice Spikelet number Protein degradation 

Supplementary material

344_2016_9579_MOESM1_ESM.pdf (152 kb)
Supplementary material 1 (PDF 152 kb)
344_2016_9579_MOESM2_ESM.pdf (38 kb)
Supplementary material 2 (PDF 38 kb)
344_2016_9579_MOESM3_ESM.pdf (23 kb)
Supplementary material 3 (PDF 23 kb)
344_2016_9579_MOESM4_ESM.pdf (65 kb)
Supplementary material 4 (PDF 64 kb)
344_2016_9579_MOESM5_ESM.xlsx (86 kb)
Supplementary material 5 (XLSX 86 kb) Fragment sequence and the individual score of each protein in this paper

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Chengqiang Ding
    • 1
  • Yan Wang
    • 1
  • Zhongyuan Chang
    • 1
  • Siliang You
    • 1
  • Zhenghui Liu
    • 1
  • Shaohua Wang
    • 1
  • Yanfeng Ding
    • 1
  1. 1.College of AgronomyNanjing Agricultural UniversityNanjingPeople’s Republic of China

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