Abstract
Lotus corniculatus is exposed to many forms of biotic stresses, including pathogen and herbivore attack. Jasmonates (JAs) are lipid-based signaling molecules that play important roles in mediating secondary metabolite biosynthesis and plant defense responses. Here, we first characterized LcJAZ1 and LcMYC2, two key components of the JAs signaling pathway. Both LcJAZ1 and LcMYC2 mRNA accumulated quickly in response to JAs. Subcellular localization analysis showed that both LcJAZ1 and LcMYC2 are nuclear proteins. In addition, LcJAZ1 protein was degraded after JAs treatment, but LcMYC2 is not. Yeast two-hybrid, pull-down, and bimolecular fluorescence complementation assays demonstrated that LcJAZ1 interacts with LcMYC2 and AtMYC2. In addition, LcMYC2 interacts with AtJAZ1. The Arabidopsis protoplast transactivation assay revealed that LcJAZ1 acts as repressors of LcMYC2- or AtMYC2-mediated transcription. Our data indicate that the conserved JAZ-MYC complex contributes to the modulation of the JAs signaling pathway.
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This work was supported by the National Natural Science Foundation of China (Grant No. 31372361) and National Program on Key Basic Research Project (973 Program) (Grant No. 2014CB138701).
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Meiliang Zhou and Zhanmin Sun have contributed equally to this work.
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344_2015_9551_MOESM2_ESM.pdf
Supplementary material 2 . Supporting Information Figure S1: AtMYC3 and AtMYC4 interact with LcJAZ1 and AtJAZ1 in yeast. Yeast cells expressing LcJAZ1 and AtJAZ1 proteins fused to the GAL4 BD and AtMYC3 and AtMYC4 fused to the GAL4 AD were spotted on SD/LW (-2) to select for the plasmids and on SD/-LWH with 10 mM 3-AT (-3) to select for transcriptional activation of the His3 gene. Growth was monitored after 7 days. Yeast cells transformed with the empty plasmids pAS2.1 and pACT2, expressing GAL4 BD and AD, respectively, were used as controls. (PDF 41 kb)
344_2015_9551_MOESM3_ESM.pdf
Supplementary material 3. Supporting Information Figure S2: Western blot using anti-HA to identify the positive transgenic plants. W: wild-type plants. (PDF 49 kb)
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Zhou, M., Sun, Z., Li, J. et al. Identification of JAZ1-MYC2 Complex in Lotus corniculatus . J Plant Growth Regul 35, 440–448 (2016). https://doi.org/10.1007/s00344-015-9551-4
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DOI: https://doi.org/10.1007/s00344-015-9551-4