MeJA Affects Root Growth by Modulation of Transmembrane Auxin Flux in the Transition Zone
- 570 Downloads
Biosynthesis and accumulation of jasmonate (JA) can regulate plant defense responses and organ development under insect herbivore stress conditions. One of these responses, the inhibition of root growth, is a common phenomenon. However, the physiological and molecular mechanisms of how JA affects root growth are not completely understood. In this study, the influence of MeJA treatment on auxin and proton flux rates in the root apex region of Col-0, coi1-1, pin2, and aux1-7 mutant lines was examined using a non-invasive micro-test technique. The auxin and H+ flux profiles taken from coi1-1 mutants suggest that the modulation of auxin and H+ flux by JA requires the function of COI1. The auxin and H+ flux in pin2 and aux1 mutants with and without JA treatment suggest that JA can affect polar auxin transport by regulating PIN-mediated auxin efflux and the AUX-mediated influx pathways. Furthermore, the expression levels of PIN1, PIN2, PIN3, PIN7, AUX1, and TIR1 genes were reduced under MeJA treatment, and expression levels of CYP79B2 and CYP79B3 were elevated. Together, these results suggest that JA signaling may modulate the auxin signaling pathways by regulating the expression of key genes.
KeywordsAuxin flux H+ flux Jasmonate COI1 Arabidopsis thaliana
We would like to thank Professor Daoxin Xie for providing seeds of COI1 mutations coi1-1. We would like to thank Professor Hongwei Guo for providing seeds of aux1–7. We also would like to thank Xuyue (Beijing) Science and Technology Company for their technical support. This work was financially supported by the National ‘863’ Plant Project (No. 2011AA10020102) and the National Natural Science Foundation of China (31270655) and we would also like to thank Dr. Melanie Correll for proofreading the manuscript prior to submission.
Compliance with Ethical Standards
Conflict of interest
All the authors have no conflict of interest.
- Chen Q, Sun J, Zhai Q, Zhou W, Qi L, Xu L, Wang B, Chen R, Jiang H, Qi J, Li X, Palme K, Li C (2011) The basic Helix-Loop-Helix transcription factor MYC2 directly represses PLETHORA expression during Jasmonate-mediated modulation of the root stem cell niche in Arabidopsis. Plant Cell 23:3335–3352PubMedCentralCrossRefPubMedGoogle Scholar
- Petersson SV, Johansson AI, Kowalczyk M, Makoveychuk A, Wang JY, Moritz T, Grebe M, Benfey PN, Sandberg G, Ljung K (2009) An auxin gradient and maximum in the Arabidopsis root apex shown by high-resolution cell-specific analysis of IAA distribution and synthesis. Plant Cell 21:1659–1668PubMedCentralCrossRefPubMedGoogle Scholar
- Qi L, Yan J, Li Y, Jiang H, Sun J, Chen Q, Li H, Chu J, Yan C, Sun X, Yu Y, Li C, Li C (2012) Arabidopsis thaliana plants differentially modulate auxin biosynthesis and transport during defense responses to the necrotrophic pathogen Alternaria brassicicola. New Phytol 195:872–882CrossRefPubMedGoogle Scholar
- Sun J, Xu Y, Ye S, Jiang H, Chen Q, Liu F, Zhou W, Chen R, Li X, Tietz O, Wu X, Cohen JD, Palme K, Li C (2009) Arabidopsis ASA1 is important for jasmonate-mediated regulation of auxin biosynthesis and transport during lateral root formation. Plant Cell 21:1495–1511PubMedCentralCrossRefPubMedGoogle Scholar
- Taiz L, Zeiger E (2006) Plant physiology, 4th edn. Sinauer Associates, US, SunderlandGoogle Scholar
- Wen B, Bin J, Wang X (2006) Effects of methyl jasmonate and abscisic acid treatments on the hydrolysis activity of plasma membrane H+-ATPase in Mung Bean (Vigna radiata L). Plant Physiol J 42:855–859Google Scholar