Journal of Plant Growth Regulation

, Volume 35, Issue 1, pp 256–265 | Cite as

MeJA Affects Root Growth by Modulation of Transmembrane Auxin Flux in the Transition Zone

  • Suli Yan
  • Ting Zhang
  • Shanshan Dong
  • Eric Scott McLamore
  • Ningning Wang
  • Xiaoyi Shan
  • Yingbai Shen
  • Yinglang Wan
Article

Abstract

Biosynthesis and accumulation of jasmonate (JA) can regulate plant defense responses and organ development under insect herbivore stress conditions. One of these responses, the inhibition of root growth, is a common phenomenon. However, the physiological and molecular mechanisms of how JA affects root growth are not completely understood. In this study, the influence of MeJA treatment on auxin and proton flux rates in the root apex region of Col-0, coi1-1, pin2, and aux1-7 mutant lines was examined using a non-invasive micro-test technique. The auxin and H+ flux profiles taken from coi1-1 mutants suggest that the modulation of auxin and H+ flux by JA requires the function of COI1. The auxin and H+ flux in pin2 and aux1 mutants with and without JA treatment suggest that JA can affect polar auxin transport by regulating PIN-mediated auxin efflux and the AUX-mediated influx pathways. Furthermore, the expression levels of PIN1, PIN2, PIN3, PIN7, AUX1, and TIR1 genes were reduced under MeJA treatment, and expression levels of CYP79B2 and CYP79B3 were elevated. Together, these results suggest that JA signaling may modulate the auxin signaling pathways by regulating the expression of key genes.

Keywords

Auxin flux H+ flux Jasmonate COI1 Arabidopsis thaliana 

Notes

Acknowledgments

We would like to thank Professor Daoxin Xie for providing seeds of COI1 mutations coi1-1. We would like to thank Professor Hongwei Guo for providing seeds of aux17. We also would like to thank Xuyue (Beijing) Science and Technology Company for their technical support. This work was financially supported by the National ‘863’ Plant Project (No. 2011AA10020102) and the National Natural Science Foundation of China (31270655) and we would also like to thank Dr. Melanie Correll for proofreading the manuscript prior to submission.

Compliance with Ethical Standards

Conflict of interest

All the authors have no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Suli Yan
    • 1
    • 2
  • Ting Zhang
    • 1
    • 2
  • Shanshan Dong
    • 1
    • 2
  • Eric Scott McLamore
    • 3
  • Ningning Wang
    • 1
    • 2
  • Xiaoyi Shan
    • 1
  • Yingbai Shen
    • 1
    • 2
  • Yinglang Wan
    • 1
  1. 1.College of Biological Sciences and TechnologyBeijing Forestry UniversityBeijingChina
  2. 2.National Engineering Laboratory for Tree BreedingBeijing Forestry UniversityBeijingChina
  3. 3.Agricultural and Biological Engineering DepartmentUniversity of FloridaGainesvilleUSA

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