Journal of Plant Growth Regulation

, Volume 35, Issue 1, pp 163–171 | Cite as

Increase in Salt Tolerance of Arabidopsis thaliana by TaDi19

  • Yonggang Fan
  • Sule Zhang
  • Yaoyao Meng
  • Zhanjing Huang


The gene expression profile chip of salt-resistant wheat mutant RH8706-49 under salt stress was investigated. The overall length of the cDNA sequence of the probe was obtained using electronic cloning and RT-PCR. An unknown gene induced by salt was obtained, cloned, and named TaDi19 (Triticum aestivum drought-induced protein). No related report or research on the protein is available. qPCR analysis showed that gene expression was induced by many stresses, such as salt. Arabidopsis thaliana was genetically transferred using the overexpressing gene, which increased its salt tolerance. After salt stress, the transgenic plant demonstrated better physiological indicators (higher Ca2+ and lower Na+) than those of the wild-type plant. Results of non-invasive micro-test technology indicate that TaDi19-overexpressing A. thaliana significantly effluxed Na+ after salt treatment, whereas the wild-type plant influxed Na+. Chelating extracellular Ca2+ resulted in insignificant differences in salt tolerance between overexpressing and wild-type A. thaliana. Subcellular localization showed that the gene encoding protein was mainly located in the cell membrane and nucleus. TaDi19 was overexpressed in wild-type A. thaliana, and the transgenic lines were more salt-tolerant than the control A. thaliana. Thus, the wheat gene TaDi19 could increase the salt tolerance of A. thaliana.


Arabidopsis thaliana Non-invasive micro-test technology Salt stress Subcellular location Wheat 



This study was supported by the National Natural Science Fund (30971766) and the Hebei Provincial Natural Science Fund (No. C2009000278).

Supplementary material

344_2015_9513_MOESM1_ESM.pdf (1.4 mb)
Supplementary material 1 (PDF 1447 kb)


  1. Adie BA, Perez-Perez J, Perez-Perez MM et al (2007) ABA is an essential signal for plant resistance to pathogens affecting JA biosynthesis and the activation of defenses in Arabidopsis. Plant Cell 19(5):1665–1681PubMedCentralCrossRefPubMedGoogle Scholar
  2. Allakhverdiev SI, Sakamoto A, Nishiyama Y, Inaba M, Murata N (2000) Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp. Plant Physiol 123:1047–1056PubMedCentralCrossRefPubMedGoogle Scholar
  3. Antoine AF, Faure JE, Dumas C, Feijó JA (2001) Differential contribution of cytoplasmic Ca2+ and Ca2+ influx to gamete fusion and egg activation in maize. Nat Cell Biol 3(12):1120–1123CrossRefPubMedGoogle Scholar
  4. Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408(6814):796CrossRefGoogle Scholar
  5. Arnon DI (1949) Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15PubMedCentralCrossRefPubMedGoogle Scholar
  6. Assmann SM (1993) Signal transduction in guard cells. Annu Rev Cell Biol 9:345–375CrossRefPubMedGoogle Scholar
  7. Bohnert HJ, Nelson DE, Jensen RG (1995) Adaptations to environmental stresses. Plant Cell 7:1099–1111PubMedCentralCrossRefPubMedGoogle Scholar
  8. Chinnusamy V, Jagendorf A, Zhu J-K (2005) Understanding and improving salt tolerance in plants. Crop Sci 45:437–448CrossRefGoogle Scholar
  9. Clough SL, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743CrossRefPubMedGoogle Scholar
  10. Flowers TJ (2004) Improving crop salt tolerance. J Exp Bot 396:307–319CrossRefGoogle Scholar
  11. Ge RC, Chen GP, Zhao BC (2007) Cloning and functional characterization of a wheat serine/threonine kinase gene (TaSTK) related to salt-resistance. Plant Sci 173:55–60CrossRefGoogle Scholar
  12. Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid perosidation. Arch Biochem Biophys 125:189–198CrossRefPubMedGoogle Scholar
  13. Hirayama T, Shinozaki K (2007) Perception and transduction of abscisic acid signals: keys to the function of the versatile plant hormone ABA. Trends Plant Sci 12(8):343–351CrossRefPubMedGoogle Scholar
  14. Ishitani M, Xiong L, Stevenson B, Zhu JK (1997) Genetic analysis of osmotic and cold stress signal transduction in Arabidopsis: interactions and convergence of abscisic acid-dependent and abscisic acid-independent pathways. Plant Cell 9:1935–1949PubMedCentralCrossRefPubMedGoogle Scholar
  15. Kader MA, Seidel T, Golldack D, Lindberg S (2006) Expressions of OsHKT1, OsHKT2, and OsVHA are differentially regulated under NaCl stress in salt-sensitive and salt-tolerant rice (Oryza sativa L.) cultivars. J Exp Bot 57:4257–4268CrossRefPubMedGoogle Scholar
  16. Leyman B, Geelen D, Quintero FJ, Blatt MR (1999) A tobacco syntaxin with a role in hormonal control of guard cell ion channels. Science 283:537–540CrossRefPubMedGoogle Scholar
  17. MacRobbie EAC (1998) Signal transduction and ion channels in guard cells. Philos Trans R Soc Lond B 353:1475–1488CrossRefGoogle Scholar
  18. Mendoza I, Rubio F, Rodriguez-Navarro A, Prado JM (1994) The protein phosphatase calcineurin is essential for NaCl tolerance of Saccharomyces cerevisiae. Biol Chem 269:8792–8796Google Scholar
  19. Sergey S, Cuin Tracey A (2008) Potassium transport and plant salt tolerance. Physiol Plant 133(4):651–669CrossRefGoogle Scholar
  20. Shinozaki K, Yamaguchi-Shinozaki K (2000) Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways. Curr Opin Plant Biol 3:217–223CrossRefPubMedGoogle Scholar
  21. Sun J et al (2009) NaCl-induced alternations of cellular and tissue ion fluxes in roots of salt-resistant and salt-sensitive poplar species. Plant Physiol 149:141–1153Google Scholar
  22. Xiong L, Lee B, Ishitani M, Lee H, Zhang C, Zhu JK (2001) FIERY1 encoding an inositol polyphosphate 1-phosphatase is a negative regulator of abscisic acid and stress signaling in Arabidopsis. Genes Dev 15:1971–1984PubMedCentralCrossRefPubMedGoogle Scholar
  23. Zhao Q, Zhao YJ, Zhao BC (2009) Cloning and functional analysis of wheat V–H + -ATPase subunit genes. Plant Mol Biol 69:33–46CrossRefPubMedGoogle Scholar
  24. Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273PubMedCentralCrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Yonggang Fan
    • 1
  • Sule Zhang
    • 1
  • Yaoyao Meng
    • 1
  • Zhanjing Huang
    • 1
  1. 1.College of Life ScienceHebei Normal UniversityShijiazhuangPeople’s Republic of China

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