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A Time-Course Proteomic Analysis of Rice Triggered by Plant Activator BTH

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Abstract

Benzothiadiazole (BTH) is an artificial inducer of systemic acquired resistance. Due to rice being an important food crop and model plant, we investigated its response to BTH using label-free proteomics technology coupled with bioinformatics. Protein expression levels were verified using the multi-reaction monitoring mode and semi-quantitative RT-PCR. BTH treatment can up- or down-regulate many proteins produced by the rice host at all four periods, with the numbers of proteins being 6/24, 9/10, 14/10, and 8/20, respectively. Compared with mock treatments (phosphate buffered saline with 0.1 % dimethylsulfoxide and 0.5 % Tween-20), some proteins related to plant resistance were only detected after BTH treatments, such as ascorbate peroxidase (POD) 3, chitinase A, thioredoxin-dependent POD 2, beta-1,3-glucanase 2, POD superfamily protein, major facilitator superfamily (MFS) protein, copper/zinc-superoxide dismutase (SOD) 1, pathogenesis-related protein (PR) 1. Other proteins showing up-regulation after BHT treatment included PR-5, glyceraldehyde-3-phosphate dehydrogenase C, plasma-membrane associated cation-binding protein 1, and oxidoreductase family proteins. These results indicated that BTH was involved with inducing rice resistance. Some up-regulated proteins were also involved in other metabolic processes. The activity and expression level of POD, phenylalanine ammonia-lyase (PAL), and SOD, lipoxygenase (LOX), beta-1,3-glucanases, and chitinases were determined using the enzyme activity assay and semi-quantitative RT-PCR. These results indicated that BTH can enhance the activity of beta-1,3-glucanases, LOX, PAL, and POD. BTH can also induce up-regulation of the copper/zinc-SOD, ascorbate POD, glutathione POD 1, Chitinase, and LOX1 genes.

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Acknowledgments

The present work was supported by the National Natural Science Foundation of China (No. 31360446), the National Key Technologies R&D Program of China (2011BAE06B02), the Program for New Century Excellent Talents in University (NCET-13-0748), the Scientific and Technological Personnel Training Program of Guizhou Province (No. 201341), and the Agricultural Program of Science and Technology of Guizhou Province (No. 20113052).

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Authors and Affiliations

  1. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People’s Republic of China

    Zhuo Chen, Binghua Chen & Qin Guo

  2. State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Guizhou University, Guiyang, 550025, People’s Republic of China

    Zhuo Chen, Binghua Chen, Qin Guo, Li Shi, Ming He, Zhiyang Qin, Lianhua Li, Peng He, Zhenchao Wang, Deyu Hu & Song Yang

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  1. Zhuo Chen
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Chen, Z., Chen, B., Guo, Q. et al. A Time-Course Proteomic Analysis of Rice Triggered by Plant Activator BTH. J Plant Growth Regul 34, 392–409 (2015). https://doi.org/10.1007/s00344-015-9476-y

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