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Cross-Talk Between Calcium–Calmodulin and Brassinolide for Fungal Endophyte-Induced Volatile Oil Accumulation of Atractylodes lancea Plantlets

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An Erratum to this article was published on 29 May 2014

Abstract

Using pharmacological and biochemical approaches, the signalling pathways between calcium (Ca2+)–calmodulin (CaM), brassinolide (BL), and nitric oxide (NO) for fungal endophyte-induced volatile oil accumulation were investigated in Atractylodes lancea plantlets. Gilmaniella sp. AL12 inoculation elevated the concentrations of BL, CaM, and [Ca2+]cyt, expression of the calmodulin 1 (CaM1) gene, and the levels of volatile oils. Treatment with AL12 or exogenous BL led to significant increases in the levels of cytosolic Ca2+ and CaM and CaM1 expression in plantlets. However, the upregulation of BL was almost completely blocked by pretreatments with CaM antagonists and Ca2+ channel blockers. Pretreatment with a BL inhibitor, brassinazole (BRz), did not influence the increase in levels of CaM induced by the endophyte. CaCl2-induced increases in NO generation, CaM antagonists, and Ca2+ channel blockers were able to suppress NO production, and the NO-specific scavenger was not able to suppress the generation of [Ca2+]cyt in plantlets. Exogenous BL was not able to induce NO generation, and BRz had no effect on NO generation. Our results suggest that Ca2+–CaM induced by this endophyte mediates NO generation and BL concentration, and also functions downstream of BL signalling, resulting in the upregulation of volatile oil accumulation in A. lancea plantlets.

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Acknowledgments

We are grateful to the National Natural Science Foundation of China (NSFC, Nos. 31070443 and 30970523). We also express our great thanks to the reviewers and editorial staff for their time and attention.

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  1. Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences in Nanjing Normal University, Nanjing, 210023, Jiangsu Province, China

    Cheng-Gang Ren, Yan Chen & Chuan-Chao Dai

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  1. Cheng-Gang Ren
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  2. Yan Chen
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Correspondence to Chuan-Chao Dai.

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Ren, CG., Chen, Y. & Dai, CC. Cross-Talk Between Calcium–Calmodulin and Brassinolide for Fungal Endophyte-Induced Volatile Oil Accumulation of Atractylodes lancea Plantlets. J Plant Growth Regul 33, 285–294 (2014). https://doi.org/10.1007/s00344-013-9370-4

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