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Haem Oxygenase-1 is Involved in Hydrogen Sulfide-induced Cucumber Adventitious Root Formation

Journal of Plant Growth Regulation volume 31, pages 519–528 (2012)Cite this article

Abstract

Results from our previous study suggested that haem oxygenase-1/carbon monoxide (HO-1/CO) acts as a downstream signal system in the auxin-induced pathway leading to cucumber (Cucumis sativus) adventitious root formation. The objective of this study was to test whether HO-1 is also involved in hydrogen sulfide (H2S)-induced adventitious root formation. Cucumber explants were treated with HO-1 inducer haemin and H2S donor sodium hydrosulfide (NaHS) in combination with the specific inhibitor of HO-1 zinc protoporphyrin IX (ZnPPIX), and their effects on cucumber adventitious root development in IAA-depleted explants were compared. The results showed that similar to inducible responses of haemin, NaHS brought about the induction of cucumber HO-1 transcripts (CsHO-1) and its protein levels, and thereafter adventitious root formation. A further experiment verified that H2S or HS- rather than other sulfur-containing components derived from NaHS was ascribed to the stimulation response. The inducible effect is specific for CsHO-1 because ZnPPIX significantly suppressed the above responses, and the inhibitory effects were reversed partially when 30% CO-saturated aqueous solution was added. Molecular evidence further suggested that the NaHS-triggered upregulation of target genes responsible for HO-1/CO-induced adventitious root formation, including CsDNAJ-1 and CsCDPK1/5, was inhibited significantly by ZnPPIX. These decreases were reversed obviously by the addition of CO aqueous solution. However, hypotaurine (HT), the H2S scavenger, could not influence the haemin- and CO-induced adventitious rooting in IAA-depleted cucumber explants. Together, the above results suggested that HO-1 was involved in H2S-induced cucumber adventitious root formation.

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Acknowledgments

This work was supported by the Natural Science Foundation of Jiangsu Province of China (Grant No. BK2009309) and the Doctoral Fund of Ministry of Education of China (Grant No. 20100097110019).

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Authors and Affiliations

  1. College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique, Nanjing Agricultural University and Beckman Coulter Ltd. Co., Nanjing Agricultural University, Nanjing, 210095, China

    Yu-Ting Lin, Mei-Yue Li, Wei-Ti Cui, Wei Lu & Wen-Biao Shen

  2. College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People’s Republic of China

    Wen-Biao Shen

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  1. Yu-Ting Lin
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Correspondence to Wen-Biao Shen.

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Lin, YT., Li, MY., Cui, WT. et al. Haem Oxygenase-1 is Involved in Hydrogen Sulfide-induced Cucumber Adventitious Root Formation. J Plant Growth Regul 31, 519–528 (2012). https://doi.org/10.1007/s00344-012-9262-z

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  • DOI: https://doi.org/10.1007/s00344-012-9262-z

Keywords

  • Adventitious rooting
  • Cucumber
  • Haem oxygenase-1
  • Hydrogen sulfide
  • Molecular mechanism