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Application of Digital Image Analysis System for Fine Evaluation of Varietal Differences and the Role of Ethylene in Visible Petal Senescence of Morning Glory

Abstract

We detected differences in both onset and progression of visible petal senescence among morning glory cultivars by application of a digital image analysis system. The system is based on semiautomated time-lapse measurement of corolla areas. The system could also be applied to evaluate the effects of ethylene and its inhibitor on visible petal senescence. Both onset and progression of visible petal senescence were accelerated by ethylene treatment in all six cultivars tested. Treatment with aminooxyacetic acid (AOA), an ethylene biosynthesis inhibitor, prolonged time to onset of visible petal senescence in three of the six tested cultivars. In contrast, AOA treatment had no effect on duration of visible petal senescence in any tested cultivars. These data suggested differences among morning glory cultivars in the role of endogenous ethylene in controlling onset of visible petal senescence. In addition, we propose a new application of image analysis to fine quantification of time-lapse changes in the shape of plant organs.

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Acknowledgments

The authors thank Dr. Eiji Nitasaka and the National BioResource Project, Japan, for providing seeds of Q61, Q62, Q65, Q438, and Q1065, and Dr. Kenichi Shibuya for his technical assistance with ethylene measurement. This work was supported by a Japan Society for the Promotion of Science Grant-in-Aid for Young Scientists (Start-up) (no. 18880009 to T.Y.) and by a Ministry of Education, Culture, Sports, Science and Technology Grant-in-Aid for Young Scientists (B) (no. 19780019 to T.Y.).

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Correspondence to Tetsuya Yamada.

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Shinozaki, Y., Tanabata, T., Ogiwara, I. et al. Application of Digital Image Analysis System for Fine Evaluation of Varietal Differences and the Role of Ethylene in Visible Petal Senescence of Morning Glory. J Plant Growth Regul 30, 229–234 (2011). https://doi.org/10.1007/s00344-010-9182-8

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Keywords

  • Flower longevity
  • Ipomoea nil
  • Morphological character
  • Phenotypic variation
  • Quantitative determination
  • Shape analysis