Rapid Kinetic Analysis of Ethylene Growth Responses in Seedlings: New Insights into Ethylene Signal Transduction
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Ethylene is a phytohormone that influences diverse processes in plants. Ethylene causes various changes in etiolated seedlings that differ between species and include reduced growth of shoots and roots, increased diameter of shoots, agravitropic growth, initiation of root hairs, and increased curvature of the apical hook. The inhibition of growth in etiolated seedlings has become widely used to screen for and identify mutants. This approach has led to an increased understanding of ethylene signaling. Most studies use end-point analysis after several days of exposure to assess the effects of ethylene. Recently, the use of time-lapse imaging has re-emerged as an experimental method to study the rapid kinetics of ethylene responses. This review outlines the historical use of ethylene growth kinetic studies and summarizes the recent use of this approach coupled with molecular biology to provide new insights into ethylene signaling.
KeywordsEthylene Growth kinetics Signal transduction Time-lapse imaging Receptors
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- Abeles FB, Morgan PW, Saltveit ME Jr. 1992. Ethylene in Plant Biology, 2nd ed. San Diego, CA, USA, Academic Press, p 414Google Scholar
- Alonso JM, Hirayama T, Roman G, Nourizadeh S, Ecker Jr. 1999. EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis. Science 284:2148–2152Google Scholar
- Binder BM, Bleecker AB. 2003. A model for ethylene receptor function and 1-methylcyclopropene action. ACTA Hort 628:177–187Google Scholar
- Darwin C, Darwin F. 1880. The Power of Movement in Plants. London, UK, John Murray, p 592Google Scholar
- Heisenberg W. 1958. In Physics and Philosophy, Chapter III. The Copenhagen Interpretation of Quantum Theory. New York, NY, USA, Harper and Brothers. p. 58Google Scholar
- Jackson MB. 1983. Regulation of root growth and morphology by ethylene and other externally applied growth substances. In Jackson MB, Stead AD, editors. Growth Regulators in Root Development. Monograph No. 10, British Plant Growth Regulator Group, London, UK, p 103–116Google Scholar
- Mattoo AK, Suttle JC, eds. 1991. The Plant Hormone Ethylene. Boca Raton, FL, USA, CRC Press, p 337Google Scholar
- Neljubov D. 1901. Uber die horizontale Nutation der Stengel von Pisum sativum und einiger Anderer. Pflanzen Beih Bot Zentralb 10:128–139Google Scholar
- Sanders IO, Harpham NVJ, Raskin I, Smith AR, Hall MA. 1991. Ethylene binding in wild type and mutant Arabidopsis thaliana (L.) Heynh. Ann Bot 68:97–103Google Scholar
- van der Laan PA. 1934. Der Einfluss von Aethylen auf die Wuchsstoffbildung be avena und Vicia. Rec Trav Bot Neerl 31:691–742Google Scholar