Skip to main content
SpringerLink
Log in

Regenerative Xylem in Inflorescence Stems of Arabidopsis thaliana

  • Published:
Journal of Plant Growth Regulation Aims and scope Submit manuscript

Abstract

By inserting entomological needles into the lower parts of young inflorescence stems of three-month-old Arabidopsis thaliana (L.) Heynh var. Colombia plants, we studied the process of regenerative xylem production. Regenerative xylem was formed only in one- to two-day-old inflorescence stems but not in older ones. The regenerative vessels originated from re-differentiation of cortical parenchyma. To characterize the process of regenerative xylem formation, we conducted a histological study from the time of wounding to day 30 after wounding. In the first day after wounding the tissues showed no structural responses except for the wounding itself. After six days, regenerative vessel members were already differentiating in a basipetal pattern, forming a vascular bypass around the wound. Regenerative vessel member formation reached a maximal level on the twelfth day after wounding. Sixteen days after wounding the pith parenchyma started to become loose as if indicating tissue senescence. Altogether, vascular regeneration following wounding in inflorescence stems of Arabidopsis thaliana is similar to that in other dicotyledon plants. These findings provide the basis for the use of Arabidopsis thaliana as a model system to study the genetics, physiology and cell biology of wound healing and regenerative vascular tissue formation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1

Similar content being viewed by others

References

  1. O Avsian-Kretchmer J-C Cheng L Chen E Moctezuma ZR Sung (2002) ArticleTitleIndole acetic acid distribution coincides with vascular differentiation pattern during Arabidopsis leaf ontogeny. Plant Physiol 130 199–209 Occurrence Handle10.1104/pp.003228 Occurrence Handle1:CAS:528:DC%2BD38XntFOrtr4%3D Occurrence Handle12226500

    Article  CAS  PubMed  Google Scholar 

  2. EP Beers C Zhao (2001) Arabidopsis as a model for investigating gene activity and function in vascular tissues. N Morohoshi A Komamine (Eds) Molecular Breeding of Woody Plants Elsevier Science BV Amsterdam 43–52

    Google Scholar 

  3. T Berleth J Mattsson CS Hardtke (2000) ArticleTitleVascular continuity and auxin signals. Trends Plant Sci 5 387–393 Occurrence Handle1:STN:280:DC%2BD3M%2FjslKhtg%3D%3D Occurrence Handle10973094

    CAS  PubMed  Google Scholar 

  4. N Chaffey (2002) ArticleTitleWhy is there so little research into the cell biology of the secondary vascular system of trees? New Phytol 153 213–223 Occurrence Handle10.1046/j.0028-646X.2001.00311.x

    Article  Google Scholar 

  5. N Chaffey E Cholewa S Regan B Sundberg (2002) ArticleTitleSecondary xylem development in Arabidopsis: a model for wood formation. Physiol Plant 114 594–600 Occurrence Handle10.1034/j.1399-3054.2002.1140413.x Occurrence Handle1:CAS:528:DC%2BD38XjvFCnt7k%3D Occurrence Handle11975734

    Article  CAS  PubMed  Google Scholar 

  6. S Fink (1999) Pathological and regenerative plant anatomy. Gebrüder Borntraeger Berlin

    Google Scholar 

  7. V Funk B Kositsup C Zhao EP Beers (2002) ArticleTitleThe Arabidopsis xylem peptidase XCP1 is a tracheary element vacuolar protein that may be a papain ortholog. Plant Physiol 128 84–94 Occurrence Handle1:CAS:528:DC%2BD38XmvVSktQ%3D%3D Occurrence Handle11788755

    CAS  PubMed  Google Scholar 

  8. L Gälweiler C Guan A Müller E Wisman K Mendgen A Yephremov K Palme (1998) ArticleTitleRegulation of polar auxin transport by AtPIN1 in Arabidopsis vascular tissue. Science 282 2226–2230 Occurrence Handle9856939

    PubMed  Google Scholar 

  9. WP Jacobs (1952) ArticleTitleThe role of auxin in differentiation of xylem around a wound. Am J Bot 39 301–309 Occurrence Handle1:CAS:528:DyaG38XlsV2ntQ%3D%3D

    CAS  Google Scholar 

  10. WP Jacobs (1998) ArticleTitleHormone transport and action in the green shoot: long-term studies of a clonal stock of Coleus blumei (Labiatae). Am J Bot 85 1353–1363 Occurrence Handle1:CAS:528:DyaK1cXntlKktLw%3D

    CAS  Google Scholar 

  11. S Lev-Yadun (1994) ArticleTitleInduction of sclereid differentiation in the pith of Arabidopsis thaliana (L.) Heynh. J Exp Bot 45 1845–1849 Occurrence Handle1:CAS:528:DyaK2MXjsFeqsrY%3D

    CAS  Google Scholar 

  12. S Lev-Yadun (1997) ArticleTitleFibres and fibre-sclereids in wild-type Arabidopsis thaliana. Ann Bot 80 125–129 Occurrence Handle10.1006/anbo.1997.0419

    Article  Google Scholar 

  13. S Lev-Yadun (2000) Cellular patterns in dicotyledonous woods: their regulation. R Savidge J Barnett R Napier (Eds) Cell and Molecular Biology of Wood Formation Oxford BIOS Scientific Publishers Ltd 315–324

    Google Scholar 

  14. S Lev-Yadun (2002) ArticleTitleThe distance to which wound effects influence the structure of secondary xylem of decapitated Pinus pinea. JPGR 21 191–196 Occurrence Handle10.1007/s003440010057 Occurrence Handle1:CAS:528:DC%2BD38XltlOisLk%3D

    Article  CAS  Google Scholar 

  15. S Lev-Yadun R Aloni (1990) ArticleTitlePolar patterns of periderm ontogeny, their relationship to leaves and buds, and the control of cork formation. IAWA Bull n s 11 289–300

    Google Scholar 

  16. S Lev-Yadun R Aloni (1992) ArticleTitleExperimental induction of dilatation meristem in Melia azedarach. Ann Bot 70 379–386

    Google Scholar 

  17. S Lev-Yadun R Aloni (1995) ArticleTitleDifferentiation of the ray system in woody plants. Bot Rev 61 45–88

    Google Scholar 

  18. S Lev-Yadun MA Flaishman (2001) ArticleTitleThe effect of submergence on ontogeny of cambium and secondary xylem and on fiber lignification in inflorescence stems of Arabidopsis. IAWA J 22 159–169

    Google Scholar 

  19. CHA Little RA Savidge (1987) ArticleTitleThe role of plant growth regulators in forest tree cambial growth. JPGR 6 137–169 Occurrence Handle1:CAS:528:DyaL1cXnt1Sitw%3D%3D

    CAS  Google Scholar 

  20. CHA Little JE MacDonald O Olsson (2002) ArticleTitleInvolvement of indole-3-acetic acid in fascicular and interfascicular cambial growth and interfascicular extraxylary fiber differentiation in Arabidopsis thaliana inflorescence stems. Int J Plant Sci 163 519–529 Occurrence Handle10.1086/339642 Occurrence Handle1:CAS:528:DC%2BD38XlslCmtrg%3D

    Article  CAS  Google Scholar 

  21. J Mattsson ZR Sung T Berleth (1999) ArticleTitleResponses of plant vascular systems to auxin transport inhibition. Development 126 2979–2991 Occurrence Handle1:CAS:528:DyaK1MXkvFantLY%3D Occurrence Handle10357941

    CAS  PubMed  Google Scholar 

  22. C Nishitani T Demura H Fukuda (2002) ArticleTitleAnalysis of early processes in wound-induced vascular regeneration using TED3 and ZeHB3 as molecular markers. Plant Cell Physiol 43 79–90 Occurrence Handle10.1093/pcp/pcf008 Occurrence Handle1:CAS:528:DC%2BD38XhtVyqt7w%3D Occurrence Handle11828025

    Article  CAS  PubMed  Google Scholar 

  23. P Reymond H Weber M Damond EE Farmer (2000) ArticleTitleDifferential gene expression in response to mechanical wounding and insect feeding in Arabidopsis. Plant Cell 12 707–719 Occurrence Handle1:CAS:528:DC%2BD3cXjvFyhurk%3D Occurrence Handle10810145

    CAS  PubMed  Google Scholar 

  24. LW Roberts (1960) ArticleTitleExperiments on xylem regeneration in stem wound responses in Coleus. Bot Gaz 121 201–208 Occurrence Handle10.1086/336070 Occurrence Handle1:CAS:528:DyaF3MXhvVKgtQ%3D%3D

    Article  CAS  Google Scholar 

  25. LW Roberts DE Fosket (1962) ArticleTitleFurther experiments on wound-vessel formation in stem wounds of Coleus. Bot Gaz 123 247–254 Occurrence Handle10.1086/336157 Occurrence Handle1:CAS:528:DyaF38XktlOrsb0%3D

    Article  CAS  Google Scholar 

  26. CP Romano MB Hein HJ Klee (1991) ArticleTitleInactivation of auxin in tobacco transformed with the indoleacetic acid-lysine synthetase gene of Pseudomonas savastanoi. Genes Dev 5 438–446 Occurrence Handle1:CAS:528:DyaK3MXhsl2ltL4%3D Occurrence Handle2001843

    CAS  PubMed  Google Scholar 

  27. T Sachs (1981) ArticleTitleThe control of patterned differentiation of vascular tissues. Adv Bot Res 9 151–262

    Google Scholar 

  28. T Sachs (1991) Pattern formation in plant tissues. Cambridge University Press Cambridge

    Google Scholar 

  29. RA Savidge PF Wareing (1981) Plant-growth regulators and the differentiation of vascular elements. JR Barnett (Eds) Xylem cell development. Tunbridge Wells Castle House Publications Ltd. 192–235

    Google Scholar 

  30. LE Sieburth (1999) ArticleTitleAuxin is required for leaf vein pattern in Arabidopsis. Plant Physiol 121 1179–1190 Occurrence Handle1:CAS:528:DyaK1MXotFyju7k%3D Occurrence Handle10594105

    CAS  PubMed  Google Scholar 

  31. EW Sinnott R Bloch (1945) ArticleTitleThe cytoplasmic basis of intercellular patterns in vascular differentiation. Am J Bot 32 151–156

    Google Scholar 

  32. C Zhao BJ Johnson B Kositsup EP Beers (2000) ArticleTitleExploiting secondary growth in Arabidopsis. Construction of xylem and bark cDNA libraries and cloning of three xylem endopeptidases. Plant Physiol 123 1185–1196 Occurrence Handle10.1104/pp.123.3.1185 Occurrence Handle1:CAS:528:DC%2BD3cXlt1SltL0%3D Occurrence Handle10889267

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This study was supported by grants from the Research Authority and the Faculty of Science and Science Education, University of Haifa to S. Lev-Yadun. We thank Gideon Grafi for his help and comments and two anonymous reviewers for their comments.

Author information

Authors and Affiliations

  1. Department of Fruit Trees, Institute of Horticulture, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel

    Moshe A. Flaishman & Kamelia Loginovsky

  2. Department of Biology, Faculty of Science and Science Education, University of Haifa-Oranim, Tivon 36006, Israel

    Simcha Lev-Yadun

Authors
  1. Moshe A. Flaishman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kamelia Loginovsky
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Simcha Lev-Yadun
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Simcha Lev-Yadun.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Flaishman, M.A., Loginovsky, K. & Lev-Yadun, S. Regenerative Xylem in Inflorescence Stems of Arabidopsis thaliana . J Plant Growth Regul 22, 253–258 (2003). https://doi.org/10.1007/s00344-003-0030-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00344-003-0030-y

Keywords

Search

Navigation