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Increased Endogenous Auxin Production in Arabidopsis thaliana Causes Both Earlier Described and Novel Auxin-Related Phenotypes

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Abstract

Transgenic Arabidopsis thaliana plants harboring the Agrobacterium tumefaciens phytohormone-biosynthetic genes iaaM and iaaH display an altered development indicative of elevated auxin levels. These plants exhibit an increased apical dominance, increased hypocotyl and petiole length and epinastic leaves and cotyledons. In addition, the transgenic plants display the formation of necrotic spots on leaves and bracts in soil and an altered behavior of axillary buds in tissue culture. Despite transcriptional activity of the iaaM and iaaH promoters in the vasculature of root tissue, root development of young iaaM/iaaH transgenic plants is identical to wild type. However, transgenic iaaM/iaaH plants grown under tissue culture conditions for a prolonged period display both vigorous and ectopic root growth. The level of free IAA in the rosette of the iaaM/iaaH transgenic plants is increased approximately 2-fold compared to wild type. The number of cells comprising the vascular tissue in hypocotyls and inflorescence stems of the transgenic plants is reduced and these plants exhibit a reduced basipetal polar auxin transport in the inflorescence stems. This reduced polar auxin transport probably accounts for the rapid auxin effect on the aerial part of the transgenic plants and the late effect on root development.

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Acknowledgements

We thank Gerda Lamers for assistance with the anatomical analysis, Willem Hoekert and Tomasz Baginski for assistance with the generation and analysis of the transgenic lines and Dr. Remko Offringa and Dr. Bert van der Zaal for critical reading of the manuscript. This work was sponsored through the EU INCO program ERBIC 15CT9609/4.

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Authors and Affiliations

  1. Institute of Biology, Leiden University, Clusius Laboratory, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands

    Eric van der Graaff, Kees Boot & Paul J. J. Hooykaas

  2. Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-901 83 Umea, Sweden

    Roger Granbom & Goran Sandberg

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  1. Eric van der Graaff
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  2. Kees Boot
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  3. Roger Granbom
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Correspondence to Eric van der Graaff.

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van der Graaff, E., Boot, K., Granbom, R. et al. Increased Endogenous Auxin Production in Arabidopsis thaliana Causes Both Earlier Described and Novel Auxin-Related Phenotypes . J Plant Growth Regul 22, 240–252 (2003). https://doi.org/10.1007/s00344-003-0014-y

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