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Analysis And Localization of the Water-Deficit Stress-Induced Gene (lp3)

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Abstract

LP3 is a water-deficit-induced protein, which is highly homologous to ASR (ABA, stress and ripening) proteins. Homology was found in the C-terminal region of the putative LP3 protein while lower homologies were found in the N-terminal region. The goal of this study was to investigate the function of the LP3 protein and the mechanism of the lp3 promoter in response to water-deficit stress (WDS) and other stresses. In regenerated transgenic tobacco (T0), expression of β-glucuronidase (GUS) from the lp3 promoter-GUS construct was observed in polyethylene glycol (PEG), abscisic acid (ABA), methyl-jasmonate (MeJa), and fluridone (Flu) treatments. GUS expression was not observed following gibberellin (GA3), 2-methyl-4-dichlorophenoxy acetic acid (2,4-D), silver nitrate, or ethephon (ethylene releasing agent) treatments. Germinated T1 seedlings containing the lp3 promoter-GUS construct exhibited GUS activity up to 40 days postgermination. Expression could be restored when 5-azacytidine was included in the culture media, indicative of a developmentally regulated silencing mechanism involving methylation. In transgenic tobacco, the LP3 protein localized in the cell nucleus was induced by WDS and appeared to be developmentally regulated.

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Acknowledgements

We wish to thank Dr. Hongyan Wang for assistance with plasmid construction, Mr. Jinhua Luo for guidance with plant transformation, and Ms. Kitty Sen for insight and assistance with DNA bombardment.

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Authors and Affiliations

  1. Department of Forest Science, Texas A&M University, College Station, 77843, Texas, USA

    Jau-Tay Wang, Jean H. Gould, Veera Padmanabhan & Ronald J. Newton

  2. Transworld Institute of Technology, Toulin 640, Yunlin, Taiwan, Republic of China

    Jau-Tay Wang

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  1. Jau-Tay Wang
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Correspondence to Jau-Tay Wang.

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Wang, JT., Gould, J.H., Padmanabhan, V. et al. Analysis And Localization of the Water-Deficit Stress-Induced Gene (lp3) . J Plant Growth Regul 21, 469–478 (2002). https://doi.org/10.1007/s00344-002-0128-7

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