Abstract
This chemical study reports a novel siderophore-like compound, β-cyclopiazonic acid (1, β-CPA) extracted from marine fungus Aspergillus flavus. The chemical structure of β-CPA was elucidated by a combination of extensive spectroscopic analyses and TDDFT-ECD calculations. The iron-binding ability and CAS assays demonstrate that β-CPA is a novel siderophore that features a different chemical structure from those of traditional siderophores. The β-CPA has no obvious influence on the growth of bacterium Pseudomonas aeruginosa PAO1. However, its iron chelator could promote the growth of P. aeruginosa PAO1, suggesting that P. aeruginosa employed siderophores to sequester iron, which is vital for their survival. The study provides the physiochemical evaluation of β-CPA, an unusual skeleton-structure siderophore, which for the first time, was proven to have the ability to bind iron and affect P. aeruginosa growth. This new discovery of siderophore provides an opportunity for developing novel anti-P. aeruginosa drugs.
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Data Availability Statement
All data generated and/or analyzed during this study are included in this published article and its supplementary information files.
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Supported by the Natural Foundation of Zhejiang Province (No. LGF21H300003), the Key Laboratory of Tropical Marine Ecosystem and Bioresource, Ministry of Natural Resources (No. 2021QN03), the Key Research and Development Program of Zhejiang Province (No. 2021C03084), the Key Research and Development Program of National Key R&D Program of China (Nos. 2018YFC0311003, 2017YFE0103100), and the High Level Talent Special Support Plan of Zhejiang Province (No. 2019R52009). We also gratefully acknowledge platform support from Zhejiang International Sci-Tech Cooperation Base for the Exploitation and Utilization of Nature Product
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Guo, Y., Ying, Y., Wu, Q. et al. β-cyclopiazonic acid binds iron demonstrating siderophore-like activity and promotes growth in Pseudomonas aeruginosa. J. Ocean. Limnol. 41, 1159–1167 (2023). https://doi.org/10.1007/s00343-022-2007-3
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DOI: https://doi.org/10.1007/s00343-022-2007-3