Abstract
Biofloc technology (BFT) improves water quality, and productivity of the farmed species through converting ammonium nitrogen to microbial protein, stabilizing microbial community, and reducing the production cost. In this study, a small-scale biofloc development unit was designed in combination of recirculation system (RAS) for Artemia culture. Artemia growth, water quality, and microbial composition of bioflocs in RAS were studied in comparison with in-situ batch culture (Glu). Glucose was added in RAS and Glu at C/N ratio of 10. The cultures without glucose addition, but with 50% daily water renewal (WRe) and without water renewal (NWRe) were considered as the controls. Artemia were cultured at 25 °C and salinity 30 for 24 days and fed formulated feed. The results showed that compared to the controls, Glu significantly improved the Artemia biomass, increased the biofloc volume, and reduced the content of total ammonia nitrogen (TAN), nitrite nitrogen (NO2−N) and nitrate nitrogen (NO3−N) in water column (P<0.05). In addition, RAS had similar results with Glu. High throughput sequencing analysis on biofloc microbial composition demonstrated that glucose supplement shaped the microbial community structure, and increased proportion of potential probiotic bacteria and suppressed pathogenic bacteria growth. Furthermore, we analyzed the relationship between the microbial composition of biofloc and environmental factors. Canonical correspondence analysis (CCA) indicated that inorganic nitrogen in culture water had great impact on biofloc microbial composition in NWRe and WRe, whilst the dissolved organic carbon (DOC) modified the microbial community in Glu and RAS. This study shows the advantages of BFT in Artemia culture and provides practical information for applying BFT-RAS in indoor Artemia culture.
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Data Availability Statement
The data presented in this paper can be shared upon request.
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Supported by the Yangtze Scholars and Innovative Research Team in University of Ministry of Education of China (No. IRT_17R81) and the Project of Agricultural intelligence introduction of Tibet (No. 2020WZ006)
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Liang, X., Zhang, C., Du, D. et al. Application of biofloc technology in recirculation Artemia culture system. J. Ocean. Limnol. 40, 1669–1677 (2022). https://doi.org/10.1007/s00343-021-1135-5
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DOI: https://doi.org/10.1007/s00343-021-1135-5