Abstract
The most prominent biological characteristic of Porphyridium purpureum is their unique extracellular sulfated polysaccharides that plays an important protective role against salt stress. Adaptation to stress is associated with metabolic adjustments. However, the molecular mechanisms underlying such metabolic changes remain elusive. This study presents the first transcriptome profiling of P. purpureum. A total of 8 019 assembled transcripts were identified, annotated, and classified into different biological categories and pathways based on a BLAST analysis against various databases. The in-depth analysis revealed that many of the differentially expressed genes in P. purpureum under salinities of 68 and 0 involved polysaccharide metabolism. A comparison of the gene expression levels under different salinities revealed that the polysaccharide synthetic pathway was significantly upregulated under the 68 salinity condition. The increased expression of the FBP, pfkA, CS, pgm, USP, UGP2, galE, and MPI transcripts in the polysaccharide synthetic pathway and the increase in ATP2 and ATP6 transcripts in the energy metabolic pathway revealed the molecular mechanism of high-salt adaptation. This sequencing dataset and analysis could serve as a valuable resource to study the mechanisms involved in abiotic stress tolerance in Rhodophyta.
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Data Availability Statement
The datasets generated during and analyzed during the current study are available from the corresponding author on reasonable request.
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Supported by the National Natural Science Foundation of China (Nos. 31670208 and 41871037 to Shulian XIE and 31800172 to Fangru NAN), the Applied Basic Research Project of Shanxi, China (No. 201801D221245), the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi (No. 2019L0078), and the Fund for Shanxi “1331 Project” Key Innovative Research Team
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Lu, X., Nan, F., Feng, J. et al. Transcriptome analysis of Porphyridium purpureum under salinities of 0 and 68. J. Ocean. Limnol. 40, 1600–1614 (2022). https://doi.org/10.1007/s00343-021-1076-z
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DOI: https://doi.org/10.1007/s00343-021-1076-z