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Effects of dietary cottonseed meal protein hydrolysate on growth, antioxidants and immunity of Chinese mitten crab Eriocheir Sinensis

  • Aquaculture and Fisheries
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Abstract

One hundred and sixty crabs (average initial weight: 51.32±0.08 g) were fed with four experimental diets containing cottonseed meal protein hydrolysate (CPH) at 0% (CPH0, control), 0.3% (CPH0.3), 0.6% (CPH0.6), and 1.2% CPH (CPH1.2). The experiment results show that no difference was observed in specific growth rate and survival rate of crabs fed with CPH diet. Moisture content of crabs fed with CPH0.6 diet was significantly reduced than that of the CPH0 group. Superoxide dismutase and catalase activities of crabs fed with CPH0.6 diet were significantly increased and the difference was not significant between the CPH0.3 and CPH0.6 groups. Malondialdehyde content of CPH0.3 group was significantly lower than that of the CPH0 group. Lysozyme, alkaline phosphatase, and acid phosphatase activities of CPH0.3 diet crabs were significantly higher than that of the CPH0 group. Glutamic-pyruvic transaminase activity of crabs fed with CPH0.3 diet was significantly decreased compared to the CPH0 group. The relative expression levels of Toll1, Toll2, MyD88, LITAF, and ILF-2 of crabs fed with CPH0.3 diet were significantly higher than that of the CPH0 group. The expression level of SOCS2 showed an opposite pattern. After CPH perfusion, the expression levels of SOCS2 and Toll1 in intestine at time 3 h and SOCS2 in hepatopancreas at time 18 h increased significantly to the highest value. The expression level of Toll2, MyD88, LITAF decreased at times 6 h, 6 h, 12 h, respectively, then increased gradually. Therefore, supplementation of dietary CPH could improve antioxidant capacity and immune function; the appropriate supplement dosage of CPH for crab could be 0.3%–0.6%. Furthermore, the short-term CPH stimulation could significantly increase or decline the expression levels of immune-related genes at different times after CPH perfusion.

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Data Availability Statement

The data that support the findings of this study are available from the corresponding author upon reasonable request.

References

  • Association of Official Analytical Chemists (AOAC). 1984. Official Methods of Analysis. 14 th edn. AOAO, Washington, 1018p.

    Google Scholar 

  • Bergmeyer H U, Scheibe P, Wahlefeld A W. 1978. Optimization of methods for aspartate aminotransferase and alanine aminotransferase. Clinical Chemistry, 24 (1): 58–73.

    Article  Google Scholar 

  • Chen H M, Muramoto K, Yamauchi F, Fujimoto K, Nokihara K. 1998. Antioxidative properties of histidine-containing peptides designed from peptide fragments found in the digests of a soybean protein. Journal of Agricultural and Food Chemistry, 46 (1): 49–53.

    Article  Google Scholar 

  • Chen J C, Lin M N, Ting Y Y, Lin J N. 1995. Survival, haemolymph osmolality and tissue water of Penaeus chinensis juveniles acclimated to different salinity and temperature levels. Comparative Biochemistry and Physiology Part A: Physiology, 110 (3): 253–258.

    Article  Google Scholar 

  • Chen Y L, Chen L Q, Qin J G, Ding Z L, Li M, Jiang H B, Sun S M, Kong Y Q, Li E C. 2016. Growth and immune response of Chinese mitten crab (Eriocheir sinensis) fed diets containing different lipid sources. Aquaculture Research, 47 (6): 1 984–1 995.

    Article  Google Scholar 

  • Cheng H H, Jiang G Z, Zheng X C, Xu C Y, Sun C X, Zhang D D, Liu W B. 2019. Effects of five attractants on Chinese mitten crab, Eriocher sinensis Acta Hydrobiologica Sinica, 43 (2): 395–401. (in Chinese with English abstract)

    Google Scholar 

  • Colla G, Rouphael Y, Canaguier R, Svecova E, Cardarelli M. 2014. Biostimulant action of a plant-derived protein hydrolysate produced through enzymatic hydrolysis. Frontiers in Plant Science, 5: 448.

    Article  Google Scholar 

  • Duan C, Plisetskaya E M. 1993. Nutritional regulation of insulin-like growth factor-I mRNA expression in salmon tissues. Journal of Endocrinology, 139 (2): 243–252.

    Article  Google Scholar 

  • Figueiredo-Silva C, Lemme A, Sangsue D, Kiriratnikom S. 2015. Effect of DL-methionine supplementation on the success of almost total replacement of fish meal with soybean meal in diets for hybrid tilapia (Oreochromis niloticus × Oreochromis mossambicus). Aquaculture Nutrition, 21 (2): 234–241.

    Article  Google Scholar 

  • Fu L L, Zhou G, Pan J L, Li Y H, Lu Q P, Zhou J, Li X G. 2017. Effects of Astragalus polysaccharides on antioxidant abilities and non-specific immune responses of Chinese mitten crab, Eriocheir sinensis Aquaculture International, 25 (3): 1 333–1 343.

    Article  Google Scholar 

  • Gibbs B F, Zougman A, Masse R, Mulligan C. 2004. Production and characterization of bioactive peptides from soy hydrolysate and soy-fermented food. Food Research International, 37 (2): 123–131.

    Article  Google Scholar 

  • Gui D, Liu W B, Shao X P, Xu W N. 2010. Effects of different dietary levels of cottonseed meal protein hydrolysate on growth, digestibility, body composition and serum biochemical indices in crucian carp (Carassius auratus gibelio). Animal Feed Science and Technology, 156 (3–4): 112–120.

    Article  Google Scholar 

  • Guo Z L, Qiao X, Cheng R M, Shi N N, Wang A L, Feng T T, Chen Y, Zhang F, Yu H N, Wang Y P. 2017. As-CATH4 and 5, two vertebrate-derived natural host defense peptides, enhance the immuno-resistance efficiency against bacterial infections in Chinese mitten crab, Eriocheir sinensis Fish & Shellfish Immunology, 71: 202–209.

    Article  Google Scholar 

  • Hansen A C, Rosenlund G, Karlsen Ø, Koppe W, Hemre G I. 2007. Total replacement of fish meal with plant proteins in diets for Atlantic cod (Gadus morhua L.) I - effects on growth and protein retention. Aquaculture, 272 (1-4): 599–611.

    Article  Google Scholar 

  • Hartnoll R G. 2001. Growth in Crustacea-twenty years on. Hydrobiologia, 449 (1-3): 111–122.

    Article  Google Scholar 

  • Hauton C, Brockton V, Smith V J. 2006. Cloning of a crustin-like, single whey-acidic-domain, antibacterial peptide from the haemocytes of the european lobster, Homarus gammarus, and its response to infection with bacteria. Molecular Immunology, 43 (9): 1 490–1 496.

    Article  Google Scholar 

  • He J. 2005. Population growth characteristics of the mitten crab in ecological aquaculture ponds. Reservoir Fisheries, 25 (6): 10–11, 28. (in Chinese with English abstract)

    Google Scholar 

  • Hong Y H, Yang X Z, Yan G W, Huang Y, Zuo F, Shen Y X, Ding Y, Cheng Y X. 2017. Effects of glyphosate on immune responses and haemocyte DNA damage of Chinese mitten crab, Eriocheir sinensis Fish & Shellfish Immunology, 71: 19–27.

    Article  Google Scholar 

  • Huang Y, Chen Y H, Wang Z, Wang W, Ren Q. 2014. Novel myeloid differentiation factor 88, EsMyD88, exhibits EsTube-binding activity in Chinese mitten crab. Eriocheir sinensis Developmental & Comparative Immunology, 47 (2): 298–308.

    Article  Google Scholar 

  • Jia E T, Li Z Q, Xue Y F, Jiang G Z, Li X F, Liu W B, Zhang D D. 2017. Effects of dietary fructooligosaccharide on the growth, antioxidants, immunity and disease resistance of Chinese mitten crab. Aquaculture, 481: 154–161.

    Article  Google Scholar 

  • Jiang H B, Chen L Q, Qin J G. 2018. Fishmeal replacement by soybean, rapeseed and cottonseed meals in hybrid sturgeon Acipenser baerii ♀× Acipenser schrenckii ♂. Aquaculture Nutrition, 24 (4): 1 369–1 377.

    Article  Google Scholar 

  • Kotzamanis Y P, Gisbert E, Gatesoupe F J, Infante J Z, Cahu C. 2007. Effects of different dietary levels of fish protein hydrolysates on growth, digestive enzymes, gut microbiota, and resistance to Vibrio anguillarum in European sea bass (Dicentrarchus labrax) larvae. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 147 (1): 205–214.

    Article  Google Scholar 

  • Li S, Jia Z R, Li X J, Geng X Y, Sun J S. 2014. Identification and expression analysis of lipopolysaccharide-induced TNF-alpha factor gene in Chinese mitten crab Eriocheir sinensis. Fish & Shellfish Immunology, 38 (1): 190–195.

    Article  Google Scholar 

  • Li X M, Ma Y L, Liu X J. 2007. Effect of the Lycium barbarum polysaccharides on age-related oxidative stress in aged mice. Journal of Ethnopharmacology, 111 (3): 504–511.

    Article  Google Scholar 

  • Lin Z Y, Qiao J, Zhang Y L, Guo L L, Huang H, Yan F, Li Y Y, Wang X Y. 2012. Cloning and characterisation of the SpToll gene from green mud crab, Scylla paramamosain Developmental & Comparative Immunology, 37 (1): 164–175.

    Article  Google Scholar 

  • Liu W B. 2005. Effect of Plant Protein Hydrolysates on the Growth Development of Alogynogenetic crucian and Bio-Active Valve Analysis of Plant Protein Hydrolysates. Nanjing Agricultural University, Nanjing. (in Chinese with English abstract)

    Google Scholar 

  • Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−△△Cr method. Methods, 25 (4): 402–408.

    Article  Google Scholar 

  • Lygren B, Hamre K, Waagbø R. 1999. Effects of dietary proand antioxidants on some protective mechanisms and health parameters in atlantic salmon. Journal of Aquatic Animal Health, 11 (3): 211–221.

    Article  Google Scholar 

  • Matozzo V, Gallo C, Monari M, Marin M G. 2011. Cellular and biochemical parameters in the crab Carcinus aestuarii after experimentally-induced stress: effects of bacterial injection, leg ablation and bacterial injection/leg ablation combination. Journal of Experimental Marine Biology and Ecology, 398 (1-2): 18–25.

    Article  Google Scholar 

  • Möller N P, Scholz-Ahrens K E, Roos N, Schrezenmeir J. 2008. Bioactive peptides and proteins from foods: indication for health effects. European Journal of Nutrition, 47 (4): 171–182.

    Article  Google Scholar 

  • Moure A, Domínguez H, Parajó J C. 2006. Antioxidant properties of ultrafiltration-recovered soy protein fractions from industrial effluents and their hydrolysates. Process Biochemistry, 41 (2): 447–456.

    Article  Google Scholar 

  • Muranova T A, Zinchenko D V, Kononova S V, Belova N A, Miroshnikov A I. 2017. Plant protein hydrolysates as fish fry feed in aquaculture. Hydrolysis of rapeseed proteins by an enzyme complex from king crab hepatopancreas. Applied Biochemistry and Microbiology, 53 (6): 680–687.

    Article  Google Scholar 

  • Pauly D, Zeller D. 2017. Comments on FAOs State of World Fisheries and Aquaculture (SOFIA 2016). Marine Policy, 77: 176–181.

    Article  Google Scholar 

  • Rudnick D A, Hieb K, Grimmer K F, Resh V H. 2003. Patterns and processes of biological invasion: the Chinese mitten crab in San Francisco Bay. Basic and Applied Ecology, 4 (3): 249–262.

    Article  Google Scholar 

  • Sánchez A, Pascual C, Sánchez A, Vargas-Albores F, Moullac G L, Rosas C. 2001. Hemolymph metabolic variables and immune response in Litopenaeus setiferus adult males: the effect of acclimation. Aquaculture, 198 (1-2): 13–28.

    Article  Google Scholar 

  • Satoh K. 1978. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica Chimica Acta, 90 (1): 37–43.

    Article  Google Scholar 

  • Saucedo P E, Ocampo L, Monteforte M, Bervera H. 2004. Effect of temperature on oxygen consumption and ammonia excretion in the calafia mother-of-pearl oyster, Pinctada mazatlanica (Hanley, 1856). Aquaculture, 229 (1-4): 377–387.

    Article  Google Scholar 

  • Shen W Y, Fu L L, Li W F, Zhu Y R. 2010. Effect of dietary supplementation with Bacillus subtilis on the growth, performance, immune response and antioxidant activities of the shrimp (Litopenaeus vannamei). Aquaculture Research, 41 (11): 1 691–1 698.

    Article  Google Scholar 

  • Sheridan M A, Mommsen T P. 1991. Effects of nutritional state on in vivo lipid and carbohydrate metabolism of coho salmon, Oncorhynchus kisutch General and Comparative Endocrinology, 81 (3): 473–483.

    Article  Google Scholar 

  • Song Z D, Li H Y, Wang J Y, Li P Y, Sun Y Z, Zhang L M. 2014. Effects of fishmeal replacement with soy protein hydrolysates on growth performance, blood biochemistry, gastrointestinal digestion and muscle composition of juvenile starry flounder (Platichthys stellatus). Aquaculture, 426-427: 96–104.

    Article  Google Scholar 

  • Sun R, Yue F, Qiu L M, Zhang Y, Wang L L, Zhou Z, Zhang H, Yi Q L, Song L S. 2013a. The CpG ODNs enriched diets enhance the immuno-protection efficiency and growth rate of Chinese mitten crab, Eriocheir sinensis Fish & Shellfish Immunology, 35 (1): 154–160.

    Article  Google Scholar 

  • Sun S M, Qin J G, Yu N, Ge X P, Jiang H B, Chen L Q. 2013b. Effect of dietary copper on the growth performance, non-specific immunity and resistance to Aeromonas hydrophila of juvenile Chinese mitten crab, Eriocheir sinensis Fish & Shellfish Immunology, 34 (5): 1 195–1 201.

    Article  Google Scholar 

  • Tang J W, Sun H, Yao X H, Wu Y F, Wang X, Feng J. 2012. Effects of replacement of soybean meal by fermented cottonseed meal on growth performance, serum biochemical parameters and immune function of yellow-feathered broilers. Asian-Australasian Journal of Animal Sciences, 25 (3): 393–400.

    Article  Google Scholar 

  • Wang H J, Xiang L X, Shao J Z, Jia S. 2006. Molecular cloning, characterization and expression analysis of an IL-21 homologue from. Tetraodon nigroviridis Cytokine, 35 (3-4): 126–134.

    Article  Google Scholar 

  • Wang S H, Chen J C. 2005. The protective effect of chitin and chitosan against Vibrio alginolyticus in white shrimp. Litopenaeus vannamei Fish & Shellfish Immunology, 19 (3): 191–204.

    Article  Google Scholar 

  • Wang W, Gu Z F. 2002. Rickettsia-like organism associated with tremor disease and mortality of the Chinese mitten crab. Eriocheir sinensis Diseases of Aquatic Organisms, 48 (2): 149–153.

    Article  Google Scholar 

  • Wang Y W, Deng Q Q, Song D, Wang W W, Zhou H, Wang L, Li A K. 2017. Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers. Food and Agricultural Immunology, 28 (4): 725–738.

    Article  Google Scholar 

  • Wang Y, Xiong L, Yang K J, Wu Z, Sheng X M, Tang H F, Liu X P. 2005. Effect of beta-cypermethrin on GPT and GOT activities of crucian serum. Agricultural Science & Technology, 6 (1): 20–23.

    Google Scholar 

  • Wei J J, Zhang F, Tian W J, Kong Y Q, Li Q, Yu N, Du Z Y, Wu Q Q, Qin J G, Chen L Q. 2016. Effects of dietary folic acid on growth, antioxidant capacity, non-specific immune response and disease resistance of juvenile Chinese mitten crab Eriocheir sinensis (Milne-Edwards, 1853). Aquaculture Nutrition, 22 (3): 567–574.

    Article  Google Scholar 

  • Wu J Q, Kosten T R, Zhang X Y. 2013. Free radicals, antioxidant defense systems, and schizophrenia. Progress in Neuro-Psychopharmacology and BiologicalPsychiatry, 46 (1): 200–206.

    Article  Google Scholar 

  • Xia W, Liu W B, Qiao Q S, Li G F, Zhang Y J. 2012. Effects of cottonseed meal hydrolysate on growth performance and biochemical indices of Cyprinus carpio var. Jian. Freshwater Fisheries, 42 (1): 46–51. (in Chinese with English abstract)

    Google Scholar 

  • Yang C J, Zhang J Q, Li F H, Ma H M, Zhang Q L, Priya T A J, Zhang X J, Xiang J H. 2008. A Toll receptor from Chinese shrimp Fenneropenaeus chinensis is responsive to Vibrio anguillarum infection. Fish & Shellfish Immunology, 24 (5): 564–574.

    Article  Google Scholar 

  • Yang J L, Wang L L, Huang M M, Wang L L, Gai Y C, Qiu L M, Zhang H, Song L S. 2011. An interleukin-2 enhancer binding factor 2 homolog involved in immune response from Chinese mitten crab. Eriocheir sinensis Fish & Shellfish Immunology, 30 (6): 1 303–1 309.

    Article  Google Scholar 

  • Yano T. 1996. The nonspecific immune system: humoral defense. Fish Physiology, 15: 105–157.

    Article  Google Scholar 

  • Yimit D, Hoxur P, Amat N, Uchikawa K, Yamaguchi N. 2012. Effects of soybean peptide on immune function, brain function, and neurochemistry in healthy volunteers. Nutrition, 28 (2): 154–159.

    Article  Google Scholar 

  • Yu A Q, Jin X K, Guo X N, Li S, Wu M H, Li W W, Wang Q. 2013. Two novel Toll genes (EsToll1 and EsToll2 ) from Eriocheir sinensis are differentially induced by lipopolysaccharide, peptidoglycan and zymosan. Fish & Shellfish Immunology, 35 (4): 1 282–1 292.

    Article  Google Scholar 

  • Yuan C T, Li D M, Chen W, Sun F F, Wu G H, Gong Y, Tang J Q, Shen M F, Han X D. 2007. Administration of a herbal immunoregulation mixture enhances some immune parameters in carp (Cyprinus carpio). Fish Physiology and Biochemistry, 33 (2): 93–101.

    Article  Google Scholar 

  • Zhang C N, Li X F, Xu W N, Jiang G Z, Lu K L, Wang L N, Liu W B. 2013. Combined effects of dietary fructooligosaccharide and Bacillus licheniformis on innate immunity, antioxidant capability and disease resistance of triangular bream (Megalobrama terminalis). Fish & Shellfish Immunology, 35 (5): 1 380–1 386.

    Article  Google Scholar 

  • Zhang D S, Zhang Y P, Liu B, Jiang Y, Zhou Q L, Wang J, Wang H L, Xie J, Kuang Q. 2017. Effect of replacing fish meal with fermented mushroom bran hydrolysate on the growth, digestive enzyme activity, and antioxidant capacity of allogynogenetic crucian carp (Carassius auratus gibelio). Turkish Journal of Fisheries and Aquatic Sciences, 17 (5): 1 039–1 048.

    Article  Google Scholar 

  • Zhang S, Bonami J R. 2012. Isolation and partial characterization of a new reovirus in the Chinese mitten crab, Eriocheir sinensis H Milne Edwards. Journal of Fish Diseases, 35 (10): 733–739.

    Article  Google Scholar 

  • Zhang Y, Zhao J M, Zhang H, Gai Y C, Wang L L, Li F M, Yang J L, Qiu L M, Song L S. 2010. The involvement of suppressors of cytokine signaling 2 (SOCS2) in immune defense responses of Chinese mitten crab. Eriocheir sinensis Developmental & Comparative Immunology, 34 (1): 42–48.

    Article  Google Scholar 

  • Zhao D, Song L, Liu R, Liang Z, Wang L, Sun M. 2016. The immunosuppressive effects of continuous CpG ODNs stimulation in Chinese mitten crab, Eriocheir sinensis Invertebrate Survival Journal, 13: 34–43.

    Google Scholar 

  • Zheng Y H, Pu F Y. 1997. Effect of mercury on transaminase activities of tissues in C parpio & C auratus. Journal of Southwest Agricultural University, 19 (1): 41–45. (in Chinese with English abstract)

    Google Scholar 

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Authors and Affiliations

  1. Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China

    Huihui Cheng, Wenbin Liu, Xiangyang Yuan, Erteng Jia, Dingdong Zhang & Guangzhen Jiang

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  1. Huihui Cheng
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  2. Wenbin Liu
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  3. Xiangyang Yuan
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  6. Guangzhen Jiang
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Correspondence to Guangzhen Jiang.

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Supported by the Key Projects of Jiangsu Fisheries Science and Technology (No. D2018-4) and the Aquatic Three New Project of Jiangsu Province (No. Y2016-26)

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Cheng, H., Liu, W., Yuan, X. et al. Effects of dietary cottonseed meal protein hydrolysate on growth, antioxidants and immunity of Chinese mitten crab Eriocheir Sinensis. J. Ocean. Limnol. 38, 869–882 (2020). https://doi.org/10.1007/s00343-019-9152-3

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