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Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater

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Abstract

Turbot harbor a relatively remarkable ability to adapt to opposing osmotic challenges and are an excellent model species to study the physiological adaptations of flounder associated with osmoregulatory plasticity. The kidney transcriptome of turbot treated 24 h in water of hypo-salinity(salinity 5) and seawater(salinity 30) was sequenced and characterized. In silico analysis indicated that all unigenes had significant hits in seven databases. The functional annotation analysis of the transcriptome showed that the immune system and biological processes associated with digestion, absorption, and metabolism played an important role in the osmoregulation of turbot in response to hypo-salinity. Analysis of biological processes associated with inorganic channels and transporters indicated that mineral absorption and bile secretion contributed to iono-osmoregulation resulting in cell volume regulation and cell phenotypic plasticity. Moreover, we analyzed and predicted the mechanisms of canonical signaling transduction. Biological processes involved in renin secretion, ECM-receptor interaction, adherens junction, and focal adhesion played an important role in the plasticity phenotype in hypo-stress, while the signal transduction network composed of the MAPK signaling pathway and PI3K-Akt signaling pathway with GABAergic synapse, worked in hypo-osmoregulation signal transduction in the turbot. In addition, analysis of the tissue specificity of targeted gene expression using qPCR during salinity stress was carried out. The results showed that the kidney, gill, and spleen were vital regulating organs of osmotic pressure, and the osmoregulation pattern of euryhaline fish differed among species.

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Data Availability Statement

The raw reads for the next generation sequencing data have been deposited in NCBI Sequence Read Archive (SRA) with the GenBank number SRP153594.

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Authors and Affiliations

  1. College of Fisheries and Life Science, Shanghai Ocean University, Ministry of Education, Shanghai, 201306, China

    Wenxiao Cui

  2. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China

    Wenxiao Cui, Aijun Ma, Zhihui Huang, Xin’an Wang, Zhibin Sun, Zhifeng Liu, Wei Zhang, Jingkun Yang & Jinsheng Zhang

  3. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences; Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding; Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao, 266071, China

    Wenxiao Cui, Aijun Ma, Zhihui Huang, Xin’an Wang, Zhibin Sun, Zhifeng Liu, Wei Zhang, Jingkun Yang & Jinsheng Zhang

  4. Yantai Tianyuan Aquatic Limited Corporation, Yantai, 264003, China

    Jiangbo Qu

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  1. Wenxiao Cui
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Correspondence to Aijun Ma.

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Supported by the Earmarked Fund for Modern Agro-Industry Technology Research System(No. CARS-47-G01), the AoShan Talents Cultivation Program supported by Qingdao National Laboratory for Marine Science and Technology(No. 2017ASTCP-OS04), the National Natural Science Foundation of China(No. 41706168), the Agricultural Fine Breed Project of Shandong(No. 2019LZGC013), the Basal Research Fund, Chinese Academy of Fishery Sciences(No. 2016HY-JC0301), and the Yantai Science and Technology Project(No. 2018ZDCX021)

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Cui, W., Ma, A., Huang, Z. et al. Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater. J. Ocean. Limnol. 38, 467–479 (2020). https://doi.org/10.1007/s00343-019-9056-2

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