Isolation and characterization of AHL-degrading bacteria from fi sh and pond sediment

  • Miaoyu Li
  • Bingwen XiEmail author
  • Ting Qin
  • Kai Chen
  • Jun XieEmail author


Quorum sensing (QS) disruption is considered as a potential alternative strategy to combat bacterial diseases in aquaculture. In this study, we isolated and identifi ed bacteria degrading QS molecules from pond sediment and fi sh intestine. A total of 132 strains were obtained in the enrichment culture, of which two strains were identifi ed as Enterobacter sp. f003 and Staphylococcus sp. sw120, being isolated from the fi sh intestine and pond sediment, respectively. We found that strains f003 and sw120 could degrade acyl-homoserine lactones (AHLs) and cause no hemolysis of sheep red blood cells. The AHL lactonase ( aiiA ) homologous gene in the two strains was detected in PCR amplifi cation and the high-degrading activity to N-hexanoyl-L-homoserine lactone (C6-HSL) and AHLs secreted from pathogenic Aeromonas hydrophila was assessed. Meanwhile, the artifi cial infection of cyprinid C arassius auratus gibelio with intraperitoneal injection showed that the two strains were avirulent. Therefore, the obtained indigenous bacteria are candidate probiotics against pathogenic A. hydrophila in aquaculture.

Key word

quorum sensing Aeromonas hydrophila probiotic Enterobacter Staphylococcus 


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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Wuxi Fisheries CollegeNanjing Agricultural UniversityWuxiChina
  2. 2.Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxiChina

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