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Isolation and characterization of AHL-degrading bacteria from fish and pond sediment

  • Aquaculture and Fisheries
  • Published:
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Abstract

Quorum sensing (QS) disruption is considered as a potential alternative strategy to combat bacterial diseases in aquaculture. In this study, we isolated and identified bacteria degrading QS molecules from pond sediment and fish intestine. A total of 132 strains were obtained in the enrichment culture, of which two strains were identified as Enterobacter sp. f003 and Staphylococcus sp. sw120, being isolated from the fish intestine and pond sediment, respectively. We found that strains f003 and sw120 could degrade acyl-homoserine lactones (AHLs) and cause no hemolysis of sheep red blood cells. The AHL lactonase ( aiiA ) homologous gene in the two strains was detected in PCR amplification and the high-degrading activity to N-hexanoyl-L-homoserine lactone (C6-HSL) and AHLs secreted from pathogenic Aeromonas hydrophila was assessed. Meanwhile, the artificial infection of cyprinid C arassius auratus gibelio with intraperitoneal injection showed that the two strains were avirulent. Therefore, the obtained indigenous bacteria are candidate probiotics against pathogenic A. hydrophila in aquaculture.

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Authors and Affiliations

  1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China

    Miaoyu Li & Jun Xie

  2. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China

    Miaoyu Li, Bingwen Xi, Ting Qin, Kai Chen & Jun Xie

Authors
  1. Miaoyu Li
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  2. Bingwen Xi
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  3. Ting Qin
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  4. Kai Chen
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  5. Jun Xie
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Correspondence to Bingwen Xi or Jun Xie.

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Supported by the Central Public-Interest Scientific Institution Basal Research Fund, CAFS (Nos. 2017HY-ZD1008, 2017JBFR03) and the Earmarked Fund for China Agriculture Research System (No. CARS-45)

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Li, M., Xi, B., Qin, T. et al. Isolation and characterization of AHL-degrading bacteria from fish and pond sediment. J. Ocean. Limnol. 37, 1460–1467 (2019). https://doi.org/10.1007/s00343-019-8137-6

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  • DOI: https://doi.org/10.1007/s00343-019-8137-6

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