Abstract
Blepharipoda liberate Shen is a commercially valuable seafood species that has important ecological significance in Shandong Province, China. Although B. liberate is crustacean, its external characteristics are not entirely those of shrimps or crabs. The question of whether B. liberate is a shrimp or a crab has been debated in recent years. We studied the karyotype of B. liberate by light microscopy using air-drying and spreading methods. We obtained mitotic chromosomal plates from B. liberate larvae, and from adult B. liberate females subsequent to egg-laying. The results revealed that B. liberate has 53 pairs of chromosomes (i.e., n=53 and 2n=106), a characteristic shared with four species of crab. The karyogram of B. liberate consists of 25 metacentric, 14 submetacentric, 11 subtelocentric and 3 telocentric pairs. We did not find any heteromorphosis sex chromosomes. Tissue from larvae, gills and ovaries can be used for chromosomal investigations, and we found similar lampbrush chromosomes in ovary cells. Comparatively speaking, larvae tissue is more practical, and ovary tissue is more suitable for the preparation of lampbrush chromosomes. B. liberate is more closely related to crabs than to shrimps, based on the numbers of chromosomes. The B. liberate karyotype reported here provides a basis for further comparative cytogenetic studies of species populations.
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Acknowledgement
The authors are grateful to SUN Yuzhong, Director of Rizhao Ocean & Fisheries Research Institute, and to Professor CHEN Siqing and engineer ZHANG Shengnong at the Yellow Sea Fisheries Institute, Chinese Academy of Fishery Sciences for enabling the cytogenetic experiments and chromosome preparations.
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Supported by the Shandong Province Agricultural Seed Improvement Project (No. 2013lz058), the Central Public-interest Scientifi c Institution Basal Research Fund, YSFRI, CAFS (No. 20603022018004), the Rizhao City Applied Technology Research and Development Project (No. rzkj20122009), and the Rizhao City Enhancement and Releasing Project (No. rz2013012)
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Zhou, L., Wang, X., Wu, B. et al. Karyological analysis of the sea cicada Blepharipoda liberate Shen from the Rizhao intertidal zone, China. J. Ocean. Limnol. 37, 169–175 (2019). https://doi.org/10.1007/s00343-019-7320-0
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DOI: https://doi.org/10.1007/s00343-019-7320-0