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Molecular phylogeny and Ultrastructure of a new population of Urostyla grandis (Ciliophora, Hypotrichida) from Harbin, China

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Abstract

A new population of Urostyla grandis, collected from a freshwater pond of northeastern China was investigated by living observation, silver impregnation, and molecular phylogeny based on small subunit ribosomal rRNA (SSU rRNA). Besides, its ultrastructural characteristics were investigated carefully by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) methods. Complementary descriptions of some inner ultrastructures were provided and some new organelles were also found. Especially, the oral inner microtubule system was illustrated by SEM and TEM for the first time and a new type extrusome was reported. These results may supply complement taxonomic data and ingestion mechanism on ciliates in genus Urostyla.

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Acknowledgement

The authors would like to express their sincere gratitude to the Deanship of Scientific Research at King Saud University for its funding of this Prolific Research Group (PRG-1436-24). Many thanks are due to Prof. Weibo Song and graduate students at the Laboratory of Protozoology, Ocean University of China, for their technical and institutional help.

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Author notes

  1. CAI Xinglong and CHEN Ying contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Protozoology, Harbin Normal University, Harbin, 150025, China

    Xinglong Cai, Ying Chen, Jing Gao, Lijie Yu, Xuming Pan & Zijian Qiu

  2. Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, 266003, China

    Xiaolu Zhao

  3. Zoology Department, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia

    Saleh A. Al-Farraj

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  1. Xinglong Cai
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  2. Ying Chen
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Correspondence to Xuming Pan or Zijian Qiu.

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Supported by the National Natural Science Foundation of China (Nos. 31471950, 31101613, 30970311) and the Heilongjiang Postdoctoral Funding Project “Study on toxicology of intermediate-type filament system and other ultrastructures of ciliates”

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Cai, X., Chen, Y., Zhao, X. et al. Molecular phylogeny and Ultrastructure of a new population of Urostyla grandis (Ciliophora, Hypotrichida) from Harbin, China. J. Ocean. Limnol. 37, 256–265 (2019). https://doi.org/10.1007/s00343-019-7277-z

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