Disruption of bacterial balance in the gut of Portunus trituberculatus induced by Vibrio alginolyticus infection

  • Mengjie Xia (夏梦洁)
  • Feng Pei (裴峰)
  • Changkao Mu (母昌考)
  • Yangfang Ye (叶央芳)
  • Chunlin Wang (王春琳)
Article

Abstract

Gut microbiota impacts the health of crustaceans. Vibrio alginolyticus is a main causative pathogen that induces the vibriosis in farmed swimming crabs, Portunus trituberculatus. However, it remains unknown whether gut bacteria perform functions during the progression of vibriosis. In this study, 16S rRNA gene amplicon sequencing was used to investigate temporal alteration of gut bacterial community in swimming crabs in response to 72-h V. alginolyticus challenge. Our results show that V. alginolyticus infection resulted in dynamic changes of bacterial community composition in swimming crabs. Such changes were highlighted by the overwhelming overabundance of Vibrio and a signifi cant fluctuation in the gut bacteria including the bacteria with high relative abundance and especially those with low relative abundance. These findings reveal that crab vibriosis gradually develops with the infection time of V. alginolyticus and tightly relates to the dysbiosis of gut bacterial community structure. This work contributes to our appreciation of the importance of the balance of gut bacterial community structure in maintaining the health of crustaceans.

Keywords

Portunus trituberculatus Vibrio alginolyticus gut bacterial community composition 16S rRNA gene amplicon sequencing 

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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Mengjie Xia (夏梦洁)
    • 1
    • 2
  • Feng Pei (裴峰)
    • 1
  • Changkao Mu (母昌考)
    • 1
  • Yangfang Ye (叶央芳)
    • 1
  • Chunlin Wang (王春琳)
    • 1
  1. 1.Key Laboratory of Applied Marine Biotechnology (Ningbo University)Ministry of EducationNingboChina
  2. 2.Collaborative Innovation Center for Zhejiang Marine High-Effi ciency and Healthy AquacultureNingboChina

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