Journal of Oceanology and Limnology

, Volume 36, Issue 1, pp 105–113 | Cite as

An improved method for quantitatively measuring the sequences of total organic carbon and black carbon in marine sediment cores

  • Xiaoming Xu (徐小明)
  • Qing Zhu (祝青)
  • Qianzhi Zhou (周芊至)
  • Jinzhong Liu (刘金钟)
  • Jianping Yuan (袁建平)Email author
  • Jianghai Wang (王江海)Email author


Understanding global carbon cycle is critical to uncover the mechanisms of global warming and remediate its adverse effects on human activities. Organic carbon in marine sediments is an indispensable part of the global carbon reservoir in global carbon cycling. Evaluating such a reservoir calls for quantitative studies of marine carbon burial, which closely depend on quantifying total organic carbon and black carbon in marine sediment cores and subsequently on obtaining their high-resolution temporal sequences. However, the conventional methods for detecting the contents of total organic carbon or black carbon cannot resolve the following specific difficulties, i.e., (1) a very limited amount of each subsample versus the diverse analytical items, (2) a low and fluctuating recovery rate of total organic carbon or black carbon versus the reproducibility of carbon data, and (3) a large number of subsamples versus the rapid batch measurements. In this work, (i) adopting the customized disposable ceramic crucibles with the microporecontrolled ability, (ii) developing self-made or customized facilities for the procedures of acidification and chemothermal oxidization, and (iii) optimizing procedures and carbon-sulfur analyzer, we have built a novel Wang-Xu-Yuan method (the WXY method) for measuring the contents of total organic carbon or black carbon in marine sediment cores, which includes the procedures of pretreatment, weighing, acidification, chemothermal oxidation and quantification; and can fully meet the requirements of establishing their highresolution temporal sequences, whatever in the recovery, experimental efficiency, accuracy and reliability of the measurements, and homogeneity of samples. In particular, the usage of disposable ceramic crucibles leads to evidently simplify the experimental scenario, which further results in the very high recovery rates for total organic carbon and black carbon. This new technique may provide a significant support for revealing the mechanism of carbon burial and evaluating the capacity of marine carbon accumulation and sequestration.


total organic carbon black carbon marine sediment cores chemothermal oxidation disposable ceramic crucible 


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  1. Agarwal T, Bucheli T D. 2011. Adaptation, validation and application of the chemo-thermal oxidation method to quantify black carbon in soils. Environmental Pollution, 159 (2): 532–538.CrossRefGoogle Scholar
  2. Allison L E. 1935. Organic soil carbon by reduction of chromic acid. Soil Science, 40 (4): 311–320.CrossRefGoogle Scholar
  3. Avramidis P, Nikolaou K, Bekiari V. 2015. Total organic carbon and total nitrogen in sediments and soils: a comparison of the wet oxidation-titration method with the combustion-infrared method. Agriculture and Agricultural Science Procedia, 4: 425–430.CrossRefGoogle Scholar
  4. Blair N E, Aller R C. 2012. The fate of terrestrial organic carbon in the marine environment. Annual Review of Marine Science, 4: 401–423.CrossRefGoogle Scholar
  5. Canuel E A, Hardison A K. 2016. Sources, ages, and alteration of organic matter in estuaries. Annual Review of Marine Science, 8: 409–434.CrossRefGoogle Scholar
  6. Dean Jr W E. 1974. Determination of carbonate and organic matter in calcareous sediments and sedimentary rocks by loss on ignition: comparison with other methods. Journal of Sedimentary Research, 44 (1): 242–248.Google Scholar
  7. Díaz-Zorita M. 1999. Soil organic carbon recovery by the Walkley-Black method in a typic hapludoll. Communications in Soil Science & Plant Analysis, 30 (5-6): 739–745.CrossRefGoogle Scholar
  8. Dickens A F, Gelinas Y, Masiello C A, Wakeham S, Hedges J I. 2004. Reburial of fossil organic carbon in marine sediments. Nature, 427 (6972): 336–339.CrossRefGoogle Scholar
  9. Gao X L, Yang Y W, Wang C Y. 2012. Geochemistry of organic carbon and nitrogen in surface sediments of coastal Bohai Bay inferred from their ratios and stable isotopic signatures. Marine Pollution Bulletin, 64 (6): 1148–1155.CrossRefGoogle Scholar
  10. Gillman G P, Sinclair D F, Beech T A. 1986. Recovery of organic carbon by the Walkley and Black procedure in highly weathered soils. Communications in Soil Science & Plant Analysis, 17 (8): 885–892.CrossRefGoogle Scholar
  11. Gustafsson O, Bucheli T D, Kukulska Z, Andersson M, Largeau C, Rouzaud J N, Reddy C M, Eglinton T I. 2001. Evaluation of a protocol for the quantification of black carbon in sediments. Global Biogeochemical Cycles, 15 (4): 881–890.CrossRefGoogle Scholar
  12. Hedges J I, Keil R G. 1995. Sedimentary organic matter preservation: an assessment and speculative synthesis. Marine Chemistry, 49 (2-3): 81–115.CrossRefGoogle Scholar
  13. Huang L, Zhang J, Wu Y, Wang J. 2016. Distribution and preservation of black carbon in the East China Sea sediments: Perspectives on carbon cycling at continental margins. Deep Sea Research Part II: Topical Studies in Oceanography, 124: 43–52.CrossRefGoogle Scholar
  14. Leong L S, Tanner P A. 1999. Comparison of methods for determination of organic carbon in marine sediment. Marine Pollution Bulletin, 38 (10): 875–879.CrossRefGoogle Scholar
  15. Lettens S, De Vos B, Quataert P, Van Wesemael B, Muys B, Van Orshoven J. 2007. Variable carbon recovery of Walkley-Black analysis and implications for national soil organic carbon accounting. European Journal of Soil Science, 58 (6): 1244–1253.CrossRefGoogle Scholar
  16. Lin J, Zhu Q, Hong Y H, Yuan L R, Liu J Z, Xu X M, Wang J H. 2017. Synchronous responses of sedimentary organic carbon accumulation in the inner shelf of the East China Sea to the water impoundment of Three Gorges and Gezhouba Dams. Chinese Journal of Oceanology and Limnology, Scholar
  17. Luczak C, Janquin M A, Kupka A. 1997. Simple standard procedure for the routine determination of organic matter in marine sediments. Hydrobiologia, 345 (1): 87–94.CrossRefGoogle Scholar
  18. Mantoura R F C, Martin J M, Wollast R. 1991. Ocean margin processes in global change. John Wiley and Sons Ltd.Google Scholar
  19. Meredith W, Ascough P L, Bird M I, Large D J, Snape C E, Song J, Sun Y, Tilston E L. 2013. Direct evidence from hydropyrolysis for the retention of long alkyl moieties in black carbon fractions isolated by acidified dichromate oxidation. Journal of Analytical and Applied Pyrolysis, 103: 232–239.CrossRefGoogle Scholar
  20. Mingorance M D, Barahona E, Fernández-Gálvez J. 2007. Guidelines for improving organic carbon recovery by the wet oxidation method. Chemosphere, 68 (3): 409–413.CrossRefGoogle Scholar
  21. Pohl K, Cantwell M, Herckes P, Lohmann R. 2014. Black carbon concentrations and sources in the marine boundary layer of the tropical Atlantic Ocean using four methodologies. Atmospheric Chemistry and Physics, 14 (14): 7431–7443.CrossRefGoogle Scholar
  22. Rabenhorst M C. 1988. Determination of organic and carbonate carbon in calcareous soils using dry combustion. Soils Science Society of America Journal, 52: 965–969.CrossRefGoogle Scholar
  23. Schuur E A G, McGuire A D, Schädel C, Grosse G, Harden J W, Hayes D J, Hugelius G, Koven C D, Kuhry P, Lawrence D M, Natali S M, Olefeldt D, Romanovsky V E, Schaefer K, Turetsky M R, Treat C C, Vonk J E. 2015. Climate change and the permafrost carbon feedback. Nature, 520 (7546): 171–179.CrossRefGoogle Scholar
  24. Snyder J D, Trofymow J A. 1984. A rapid accurate wet oxidation diffusion procedure for determining organic and inorganic carbon in plant and soil samples. Communications in Soil Science & Plant Analysis, 15 (5): 587–597.CrossRefGoogle Scholar
  25. Talley L D, Feely R A, Sloyan B M, Wanninkhof R, Baringer M O, Bullister J L, Carlson C A, Doney S C, Fine R A, Firing E, Gruber N, Hansell D A, Ishii M, Johnson G C, Katsumata K, Key R M, Kramp M, Langdon C, Macdonald A M, Mathis J T, McDonagh E L, Mecking S, Millero F J, Mordy C W, Nakano T, Sabine C L, Smethie W M, Swift J H, Tanhua T, Thurnherr A M, Warner M J, Zhang J Z. 2016. Changes in ocean heat, carbon content, and ventilation: A review of the first decade of GO-SHIP global repeat hydrography. Annual Review of Marine Science, 8: 185–215.CrossRefGoogle Scholar
  26. Vitti C, Stellacci A M, Leogrande R, Mastrangelo M, Cazzato E, Ventrella D. 2016. Assessment of organic carbon in soils: a comparison between the Springer-Klee wet digestion and the dry combustion methods in Mediterranean soils (Southern Italy). Catena, 137: 113–119.CrossRefGoogle Scholar
  27. Walkley A, Black I A. 1934. An examination of the Degtjareffmethod for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Science, 37 (1): 29–38.CrossRefGoogle Scholar
  28. Wang J H, Xiao X, Zhou Q Z, Xu X M, Liu J Z, Yuan D. 2018. Rates and fluxes of century-scale carbon storage in the fine-grained sediments from the central South Yellow Sea and Min-Zhe belt, East China Sea. Journal of Oceanology and Limnology, 36(1): 139–152, Scholar
  29. Wang J, Zhu L, Wang Y, Gao S, Daut G. 2012. A comparison of different methods for determining the organic and inorganic carbon content of lake sediment from two lakes on the Tibetan Plateau. Quaternary International, 250: 49–54.CrossRefGoogle Scholar
  30. Xu X M, Hong Y H, Zhou Q Z, Liu J Z, Yuan L R, Wang J H. 2017. Century-scale high-resolution black carbon records in the sediment cores from the South Yellow Sea, China. Journal of Oceanology and Limnology, 36 (1): 115–127, Scholar
  31. Yang W, Lampert D, Zhao N, Reible D, Chen W. 2012. Link between black carbon and resistant desorption of PAHs on soil and sediment. Journal of Soils and Sediments, 12 (5): 713–723.CrossRefGoogle Scholar
  32. Zhu Q, Lin J, Hong Y H, Yuan L R, Liu J Z, Xu X M, Wang J H. 2017. Century-scale records of total organic carbon in the sediment cores from the South Yellow Sea, China. Journal of Oceanology and Limnology, 36(1): 128–138, Scholar

Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Xiaoming Xu (徐小明)
    • 1
  • Qing Zhu (祝青)
    • 1
  • Qianzhi Zhou (周芊至)
    • 1
  • Jinzhong Liu (刘金钟)
    • 2
  • Jianping Yuan (袁建平)
    • 1
    Email author
  • Jianghai Wang (王江海)
    • 1
    Email author
  1. 1.Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering / South China Sea Bioresource Exploitation and Utilization Collaborative Innovation Center, School of Marine SciencesSun Yat-Sen UniversityGuangzhouChina
  2. 2.Guangzhou Institute of GeochemistryChinese Academy of SciencesGuangzhouChina

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