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Genetic mapping and QTL analysis for body weight in Jian carp (Cyprinus carpio var. Jian) compared with mirror carp (Cyprinus carpio L.)

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Abstract

We report the genetic linkage map of Jian carp (Cyprinus carpio var. Jian). An F1 population comprising 94 Jian carp individuals was mapped using 254 microsatellite markers. The genetic map spanned 1 381.592 cM and comprised 44 linkage groups, with an average marker distance of 6.58 cM. We identified eight quantitative trait loci (QTLs) for body weight (BW) in seven linkage groups, explaining 12.6% to 17.3% of the phenotypic variance. Comparative mapping was performed between Jian carp and mirror carp (Cyprinus carpio L.), which both have 50 chromosomes. One hundred and ninety-eight Jian carp marker loci were found in common with the mirror carp map, with 186 (93.94%) showing synteny. All 44 Jian carp linkage groups could be one-to-one aligned to the 44 mirror carp linkage groups, mostly sharing two or more common loci. Three QTLs for BW in Jian carp were conserved in mirror carp. QTL comparison suggested that the QTL confidence interval in mirror carp was more precise than the homologous interval in Jian carp, which was contained within the QTL interval in Jian carp. The syntenic relationship and consensus QTLs between the two varieties provide a foundation for genomic research and genetic breeding in common carp.

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Authors and Affiliations

  1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, China

    Ying Gu  (顾颖), Cuiyun Lu  (鲁翠云), Xiaofeng Zhang  (张晓峰), Chao Li  (李超) & Xiaowen Sun  (孙效文)

  2. College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai, 201306, China

    Cuiyun Lu  (鲁翠云)

  3. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China

    Juhua Yu  (俞菊华)

Authors
  1. Ying Gu  (顾颖)
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  2. Cuiyun Lu  (鲁翠云)
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  3. Xiaofeng Zhang  (张晓峰)
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  4. Chao Li  (李超)
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  5. Juhua Yu  (俞菊华)
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  6. Xiaowen Sun  (孙效文)
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Corresponding author

Correspondence to Xiaowen Sun  (孙效文).

Additional information

Supported by the Special Scientific Research Funds for Central Non-Profit Institutes, Chinese Academy of Fishery Sciences (No. 2014A05CG01), the National High Technology Research and Development Program of China (No. 2011AA100402-5), and the China Ministry of Agriculture “948” Program (No. 2011-G12)

GU Ying and LU Cuiyun contributed equally to this work.

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Gu, Y., Lu, C., Zhang, X. et al. Genetic mapping and QTL analysis for body weight in Jian carp (Cyprinus carpio var. Jian) compared with mirror carp (Cyprinus carpio L.). Chin. J. Ocean. Limnol. 33, 636–649 (2015). https://doi.org/10.1007/s00343-015-4207-6

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  • DOI: https://doi.org/10.1007/s00343-015-4207-6

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