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Effects of UV-B irradiation on isoforms of antioxidant enzymes and their activities in red alga Grateloupia filicina (Rhodophyta)

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Abstract

Macroalgae in a littoral zone are inevitably exposed to UV-B irradiance. We analyzed the effects of UV-B on isoenzyme patterns and activities of superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), and ascorbate peroxidase (APX) of red algae Grateloupia filicina (Lamour.) C. Agardh. The activities of SOD, CAT, and APX changed in response to UV-B in a time- and dose-dependent manner. POX activity increased significantly under all three UV-B treatments. The enzymatic assay showed three distinct bands of SODI (Mn-SOD), SODII (Fe-SOD), and SODIII (CuZn-SOD) under a low (Luv) and medium (Muv) dose of UV-B irradiation, while SODI and SODIII activities decreased significantly when exposed to a high dose of UV-B irradiation (Huv). The activity of POX isoenzymes increased significantly after exposure to UV-B, which is consistent with the total activity. In addition, a clear decrease in activity of CATIV was detected in response to all the three doses of UV treatments. Some bands of APX isoenzyme were also clearly influenced by UV-B irradiation. Correspondingly, the daily growth rate declined under all the three exposure doses, and was especially significant under Muv and Huv treatments. These data suggest that, although the protection mechanisms of antioxidant defense system are partly inducible by UV-B to prevent the damage, G. filicina has incomplete tolerance to higher UV-B irradiation stress.

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Authors and Affiliations

  1. College of Life Sciences, Yantai University, Yantai, 264005, China

    Jiqiang Zhao  (赵吉强) & Lixia Li  (李丽霞)

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  1. Jiqiang Zhao  (赵吉强)
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  2. Lixia Li  (李丽霞)
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Correspondence to Lixia Li  (李丽霞).

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Supported by the National Natural Science Foundation of China (No. 31300326) and the Natural Science Foundation of Shandong Province (Nos. ZR2010DQ007, ZR2011CM006)

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Zhao, J., Li, L. Effects of UV-B irradiation on isoforms of antioxidant enzymes and their activities in red alga Grateloupia filicina (Rhodophyta). Chin. J. Ocean. Limnol. 32, 1364–1372 (2014). https://doi.org/10.1007/s00343-015-3366-9

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