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Phylogeny of Saccharina and Laminaria (Laminariaceae, Laminariales, Phaeophyta) in sequence-tagged-site markers

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Abstract

Laminaria and Saccharina have recently been recognized as two independent clades from the former genus Laminaria. Traditional morphological taxonomy is being challenged by molecular evidence from both nucleus and plastid. Intensive work is in great demand from the perspective of genome colinearity. In this study, 118 sequence-tagged site (STS) markers were screened for phylogenetic analyses, 29 based on genome sequences, while 89 were based on expressed sequence tag (EST) sequences. EST-based STS marker development (29.37%) had an effi ciency twice as high as genome-sequence-based development (9.48%) as a result of high conservation of gene transcripts among the relative species. S. ochotensis, S. religiosa, S. japonica, and L. hyperborea showed great homogeneity in all 118 STS markers. Our result supports the view that the diversifi cation between the genera Saccharina and Laminaria was a more recent event and that Saccharina and Laminaria shared high phylogenetic affi nity. However, when it came to the single nucleotide polymorphism (SNP) level among the 41 SNPs, L. hyperborea owned 29 unique SNPs against 12 within the left three Saccharina species and 12 of the 13 indels were supposedly unique for L. hyperborea, indicated by its high variability. Originating from homologous ancestors, species between the recently diverged genera Laminaria and Saccharina may have taken in enough mutations at the SNP level only, in spite of different evolutionary strategies for better adaptation to the environment. Our study lays a solid foundation from a new perspective, although more accurate phylogenetic analysis is still needed to clarify the evolutionary traces between the genera Saccharina and Laminaria.

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Authors and Affiliations

  1. College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China

    Jieqiong Qu  (曲洁琼), Shan Chi  (池姗), Cui Liu  (刘翠) & Tao Liu  (刘涛)

  2. Shandong Provincial Key Laboratory of Microbial Engineering, Shandong Polytechnic University, Jinan, 250300, China

    Jing Zhang  (张静)

  3. Chinese Academy of Sciences Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100101, China

    Xumin Wang  (王绪敏)

Authors
  1. Jieqiong Qu  (曲洁琼)
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  2. Jing Zhang  (张静)
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  3. Xumin Wang  (王绪敏)
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  4. Shan Chi  (池姗)
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  5. Cui Liu  (刘翠)
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  6. Tao Liu  (刘涛)
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Correspondence to Tao Liu  (刘涛).

Additional information

Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2012AA10A406), the Science and Technology Development Project of Shandong Province, China (No. 2006GG3205001), the National Basic Scientifi c Special Fund of the Ministry of Science and Technology, China (No. 2007FY210500), the National Public Benefi t Research Foundation of the State Bureau of Oceanography, China (No. 200805075), and the Research Fund for Basic Sciences of Higher Education by National Ministry of Finance and Education, China (No. 201262003)

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Qu, J., Zhang, J., Wang, X. et al. Phylogeny of Saccharina and Laminaria (Laminariaceae, Laminariales, Phaeophyta) in sequence-tagged-site markers. Chin. J. Ocean. Limnol. 32, 22–33 (2014). https://doi.org/10.1007/s00343-014-3134-2

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  • DOI: https://doi.org/10.1007/s00343-014-3134-2

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