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First record of Biecheleria cincta (Dinophyceae) from Chinese coasts, with morphological and molecular characterization of the strains

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Abstract

The presence of Biecheleria cincta (= Woloszynskia cincta) in the Chinese coasts is reported for the first time. In scanning electron microscope, three to five series of vesicles and an elongated apical vesicle (EAV) were visible in the epicone, and both the hypocone and the cingulum had three series of vesicles each. Thin sections revealed that B. cincta possesses stalked pyrenoids and an unusual eyespot consisting of a stack of cisternae with brick-like materials (type E), thus supporting its transfer from Woloszynskia to Biecheleria. Spiny cysts formed spontaneously in culture, with an encystment rate of around 20%. Both large subunit ribosomal DNA (LSU rDNA) and internal transcribed spacer region (ITS) sequences in 12 strains from the Chinese coasts were determined. Phylogenetic analyses based on LSU rDNA and ITS sequences using Bayesian inference and maximum likelihood revealed two distinct ribotypes (referred to as ribotype A and B) in B. cincta. ITS region pairwise distances within B. cincta ranged from 0.024 to 0.072, suggesting the existence of a complex of cryptic species.

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Authors and Affiliations

  1. College of Life Science and Technology, Jinan University, Guangzhou, 510632, China

    Zhaohe Luo  (罗肇河) & Weidong Yang  (杨维东)

  2. Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China

    Bin Xu  (徐斌)

  3. Third Institute of Oceanography, SOA, Xiamen, 361005, China

    Zhaohe Luo  (罗肇河) & Haifeng Gu  (顾海峰)

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  1. Zhaohe Luo  (罗肇河)
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  2. Weidong Yang  (杨维东)
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  3. Bin Xu  (徐斌)
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  4. Haifeng Gu  (顾海峰)
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Correspondence to Haifeng Gu  (顾海峰).

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Supported by the National Scientific-Basic Special Fund (No. 2009FY210400)

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Luo, Z., Yang, W., Xu, B. et al. First record of Biecheleria cincta (Dinophyceae) from Chinese coasts, with morphological and molecular characterization of the strains. Chin. J. Ocean. Limnol. 31, 835–845 (2013). https://doi.org/10.1007/s00343-013-2315-8

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  • DOI: https://doi.org/10.1007/s00343-013-2315-8

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