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Population genetics of Sargassum horneri (Fucales, Phaeophyta) in China revealed by ISSR and SRAP markers

  • Shenhui Yu (于深辉)
  • Zhuo Chong (崇卓)
  • Fengjuan Zhao (赵凤娟)
  • Jianting Yao (姚建亭)
  • Delin Duan (段德麟)Email author
Biology

Abstract

Sargassum horneri is a common brown macro-alga that is found in the inter-tidal ecosystems of China. To investigate the current status of seaweed resources and provide basic data for its sustainable development, ISSR (inter simple sequence repeat) and SRAP (sequence related amplified polymorphism) markers were used to analyze the population genetics among nine natural populations of S. horneri. The nine studied populations were distributed over 2 000 km from northeast to south China. The percentage of polymorphic loci P % (ISSR, 99.44%; SRAP, 100.00%), Nei’s genetic diversity H (ISSR, 0.107–0.199; SRAP, 0.100–0.153), and Shannon’s information index I (ISSR, 0.157–0.291; SRAP, 0.148–0.219) indicated a fair amount of genetic variability among the nine populations. Moreover, the high degree of gene differentiation G st (ISSR, 0.654; SRAP, 0.718) and low gene flow N m (ISSR, 0.265; SRAP, 0.196) implied that there was significant among-population differentiation, possibly as a result of habitat fragmentation. The matrices of genetic distances and fixation indices (F st) among the populations correlated well with their geographical distribution (Mantel test R =0.541 5, 0.541 8; P =0.005 0, 0.002 0 and R =0.728 6, 0.641 2; P =0.001 0, 0.001 0, respectively); the Rongcheng population in the Shandong peninsula was the only exception. Overall, the genetic differentiation agreed with the geographic isolation. The fair amount of genetic diversity that was revealed in the S. horneri populations in China indicated that the seaweed resources had not been seriously affected by external factors.

Keyword

Sargassum horneri population genetics ISSR SRAP markers 

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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Shenhui Yu (于深辉)
    • 1
    • 2
  • Zhuo Chong (崇卓)
    • 1
  • Fengjuan Zhao (赵凤娟)
    • 3
  • Jianting Yao (姚建亭)
    • 1
  • Delin Duan (段德麟)
    • 1
    Email author
  1. 1.Key Laboratory of Experimental Marine Biology, Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.Graduate University of Chinese Academy of SciencesBeijingChina
  3. 3.Shandong Key Laboratory of Eco-environmental Science for Yellow River DeltaBinzhou UniversityBinzhouChina

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