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Evaluation of mouse bioassay results in an inter-laboratory comparison for paralytic shellfish poisoning toxins

  • Methods: Toxicity
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Abstract

An inter-laboratory comparison of the AOAC mouse bioassay for paralytic shellfish poisoning (PSP) toxicity in shellfish was carried out among 25 Chinese laboratories to examine the overall performance for PSP testing in China, and to analyze the main factors affecting the performance of this method. The toxic scallop Patinopecten yessoensis collected from coast of Bohai Sea, China, was used as a test sample in the comparison study. The results were reported and evaluated using robust statistical methods. The z scores showed that 80%, 8%, and 12% of laboratories reported satisfactory results, unsatisfactory results, and questionable results, respectively. This evaluation demonstrates that the PSP mouse bioassay is an appropriate method for screening and testing PSP toxicity in shellfish. However, it was found that the experience and skill of technicians, as well as the body weight and health status of mice being used significantly affected the accuracy of the method.

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Authors and Affiliations

  1. Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian, 116015, China

    Jijuan Cao  (曹际娟), Jiang Zheng  (郑江), Bing Yu  (于兵), Qiuyan Wang  (王秋艳) & Junyi Xu  (徐君怡)

  2. Key Laboratory of Marine Environment and Ecology, Ocean University of China, Ministry of Education, Qingdao, 266100, China

    Aifeng Li  (李爱峰)

Authors
  1. Jijuan Cao  (曹际娟)
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  2. Jiang Zheng  (郑江)
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  3. Bing Yu  (于兵)
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  4. Qiuyan Wang  (王秋艳)
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  5. Junyi Xu  (徐君怡)
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  6. Aifeng Li  (李爱峰)
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Corresponding author

Correspondence to Aifeng Li  (李爱峰).

Additional information

Supported by a thesis research project of General Administration of Quality Supervision, Inspection and Quarantine of China (No. 2010IK168)

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Cao, J., Zheng, J., Yu, B. et al. Evaluation of mouse bioassay results in an inter-laboratory comparison for paralytic shellfish poisoning toxins. Chin. J. Ocean. Limnol. 29, 912–916 (2011). https://doi.org/10.1007/s00343-011-0521-9

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  • DOI: https://doi.org/10.1007/s00343-011-0521-9

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