Abstract
Eutrophication, which is the enrichment of a water mass with inorganic and organic nutrients that support plant growth, is a key factor in stimulating phytoplankton growth. In this study, we determined the effects of various nitrogen sources, different nitrogen concentrations in the culture medium, and two culture methods on the growth of the green alga, Enteromorpha prolifera. The relationship between the specific growth rate of E. prolifera and NO −3 -N concentration was consistent with that estimated using the Monod equation (R 2 = 0.9713, P < 0.01). In the NO −3 -N medium, the maximum specific growth rate was calculated to be 0.1634/d and the semi-saturation constant was calculated to be 16.86 μmol/L. Our results show that E. prolifera can effectively utilize NH +4 -N, NO −3 -N, and NO −2 -N and urea-N in the range of 5 to 50 μmol/L. NH +4 -N was preferentially assimilated by E. prolifera, and urea-N was favorable for long-term growth.
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Supported by the Major Projects of Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-SW-208-01); the National Natural Science Foundation of China (No. 40976047), and National Basic Research Program of China (973 Program) (No. 2010CB428701)
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Li, J., Zhao, W. Effects of nitrogen specification and culture method on growth of Enteromorpha prolifera . Chin. J. Ocean. Limnol. 29, 874–882 (2011). https://doi.org/10.1007/s00343-011-0516-6
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DOI: https://doi.org/10.1007/s00343-011-0516-6