Impact of redox-stratification on the diversity and distribution of bacterial communities in sandy reef sediments in a microcosm

  • Zheng Gao (高峥)
  • Xin Wang (王鑫)
  • Angelos K. Hannides
  • Francis J. Sansone
  • Guangyi Wang (汪光义)
Biology

Abstract

Relationships between microbial communities and geochemical environments are important in marine microbial ecology and biogeochemistry. Although biogeochemical redox stratification has been well documented in marine sediments, its impact on microbial communities remains largely unknown. In this study, we applied denaturing gradient gel electrophoresis (DGGE) and clone library construction to investigate the diversity and stratification of bacterial communities in redox-stratified sandy reef sediments in a microcosm. A total of 88 Operational Taxonomic Units (OTU) were identified from 16S rRNA clone libraries constructed from sandy reef sediments in a laboratory microcosm. They were members of nine phyla and three candidate divisions, including Proteobacteria (Alpha-, Beta-, Gamma-, Delta-, and Epsilonproteobacteria), Actinobacteria, Acidobacteria, Bacteroidetes, Chloroflexi, Cyanobacteria, Firmicutes, Verrucomicrobia, Spirochaetes, and the candidate divisions WS3, SO31 and AO19. The vast majority of these phylotypes are related to clone sequences from other marine sediments, but OTUs of Epsilonproteobacteria and WS3 are reported for the first time from permeable marine sediments. Several other OTUs are potential new bacterial phylotypes because of their low similarity with reference sequences. Results from the 16S rRNA, gene clone sequence analyses suggested that bacterial communities exhibit clear stratification across large redox gradients in these sediments, with the highest diversity found in the anoxic layer (15–25 mm) and the least diversity in the suboxic layer (3–5 mm). Analysis of the nosZ, and amoA gene libraries also indicated the stratification of denitrifiers and nitrifiers, with their highest diversity being in the anoxic and oxic sediment layers, respectively. These results indicated that redox-stratification can affect the distribution of bacterial communities in sandy reef sediments.

Keyword

bacterial diversity bacterial stratification biogeochemical gradients sandy reef sediments 

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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Zheng Gao (高峥)
    • 1
  • Xin Wang (王鑫)
    • 2
  • Angelos K. Hannides
    • 2
  • Francis J. Sansone
    • 2
  • Guangyi Wang (汪光义)
    • 2
    • 3
  1. 1.State Key Laboratory of Crop Biology, College of Life SciencesShandong Agricultural UniversityTai’anChina
  2. 2.Department of OceanographyUniversity of HawaiiHonoluluUSA
  3. 3.Shenzhen Engineering Laboratory for Algal Biofuel Technology Development and Application, School of Environment and EnergyPeking University Shenzhen Graduate SchoolShenzhenChina

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