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Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)

Chinese Journal of Oceanology and Limnology volume 29, Article number: 1260 (2011) Cite this article

Abstract

A filamentous clone of Polysiphonia urceolata was regenerated from segments cut from the fronds of gametophytes. Unlike wild thalli with short virgate branchlets, the clone was filamentous with few branches. Many transparent trichoblasts arose from pericentral cells during the induction culture, but these were seldom observed during normal growth. The trichoblasts were uniseriate, often colorless, and formed lobed rhizoids rapidly when they came into contact with solid substrates. In addition to morphological characteristics, the photosynthetic properties and growth conditions of the clone differed from those of the mother plant. Cross-gradient light and temperature culture experiments revealed that the most favorable conditions for culture of the filamentous clone were 22°C and 95–120 μE/(m2·s) light intensity. The photosynthetic light saturation value for filaments was approx. 100 μE/(m2·s), which is far lower than that of wild thalli. These results could be used to develop techniques for mass cultures of P. urceolata in photobioreactors for production of seed stock or bioactive products.

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Jinxia Wang & Baicheng Zhou

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Jinxia Wang

  3. National Marine Environmental Monitoring Center, Dalian, 116023, China

    Kuishuang Shao

  4. Institute of Oceanology & Marine Fisheries, Nantong, 226007, China

    Bin Cheng & Qinqin Lu

Authors
  1. Jinxia Wang
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  2. Kuishuang Shao
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  3. Bin Cheng
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  4. Qinqin Lu
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  5. Baicheng Zhou
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Correspondence to Jinxia Wang.

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Supported by the National Special Research Fund for Non-Profit Sector (No. 200905020-06)

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Wang, J., Shao, K., Cheng, B. et al. Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta). Chin. J. Ocean. Limnol. 29, 1260 (2011). https://doi.org/10.1007/s00343-011-0295-0

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  • DOI: https://doi.org/10.1007/s00343-011-0295-0

Keyword

  • Polysiphonia urceolata
  • Rhodophyta
  • filament
  • culture