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Digestive enzyme activities in mudskipper Boleophthalmus pectinirostris and Chinese black sleeper Bostrichthys sinensis

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Abstract

The mudskipper Boleophthalmus pectinirostris and Chinese black sleeper Bostrichthys sinensis occupy the intertidal zone. However, both species have their own unique diet. The former is an herbivore and the latter is a carnivore. In order to reveal the relationship between digestive enzyme activities and diets in the two species, the activities of protease (P), non-specific bile salt-activated lipase (BAL) and α-amylase (A) were determined in the stomach and intestine of adult mudskipper B. pectinirostris and Chinese black sleeper B. sinensis. The results showed that the activities of protease, BAL and α-amylase in the intestine of B. pectinirostris were significantly (P<0.05) higher than those in the stomach. In B. sinensis, gastric protease activity was not different from the intestinal protease (P>0.05), while BAL and α-amylase activities of the intestine were significantly (P<0.05) higher than those of the stomach. The activity of gastric protease in B. sinensis was significantly (P<0.05) higher than that in B. pectinirostris, while the activities of intestinal protease were not different between the two fish species (P>0.05). BAL activities of the stomach and intestine in B. sinensis were significantly (P<0.05) higher than those in B. pectinirostris, while α-amylase activities of the stomach and intestine in B. pectinirostris were significantly (P<0.05) higher than those in B. sinensis. The ratios of P/BAL, A/P and A/BAL of the digestive tract in B. pectinirostris were 1.5, 107.3 and 158.6, respectively; and those in B. sinensis were 0.2, 1.6 and 0.2, respectively. It can be concluded that food digestion in the adult B. pectinirostris is mainly carried out in the intestine, whereas in the adult B. sinensis it is initiated in the stomach and finishes in the intestine. The activities of BAL and α-amylase in B. pectinirostris and B. sinensis are well correlated with their diets. However, a clear-cut correlation between protease activity and diets is not found in these two species.

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References

  • Biesiot P M, Capuzzo J M. 1990. Changes in digestive enzyme activities during early development of the American lobster Homarus americanus Milne Edwards. J. Exp. Mar. Biol. Ecol., 136(2): 107–122.

    Article  Google Scholar 

  • Buddington R K, Doroshov S I. 1986. Digestive enzyme complement of white sturgeon (Acipenser transmontanus). Comp. Biochem. Physiol., A83: 561–567.

    Article  Google Scholar 

  • Caviedes-Vidal E, Afik D, Martinez del Rio C, Karasov W H. 2000. Dietary modulation of intestinal enzymes of the house sparrow (Passer domesticus): testing an adaptive hypothesis. Comp. Biochem. Physiol., A125: 11–24.

    Google Scholar 

  • Chakrabarti I, Md A Gani, Chaki K K, Sur R, Misra K K. 1995. Digestive enzymes in 11 freshwater teleost fish species in relation to food habit and niche segregation. Comp. Biochem. Physiol., A112: 167–177.

    Article  Google Scholar 

  • Chan A S, Horn M H, Dickson K A, Gawlicka A. 2004. Digestive enzyme activities in carnivores and herbivores: comparisons among four closely related prickleback fishes (Teleostei: Stichaeidae) from a California rocky intertidal habitat. J. Fish Biol., 65:848–858.

    Article  Google Scholar 

  • Chen Q, Sternby B, Akesson B, Nilsson A. 1990. Effects of human pancreatic lipase-colipase and carboxyl ester lipase on eicosapentanoic and arachidonic acid ester bonds of triacylglycerols rich in fish oil fatty acids. Biochim. Biophys. Acta., 1 044: 111–117.

    Google Scholar 

  • Chen S X., Hong W S, Zhang Q Y, Wu R X, Wang Q. 2006. Rate of oxygen consumption and tolerance of hypoxia and desiccation in Chinese black sleeper (Bostrichthys sinensis) and mudskipper (Boleophthalmus pectinirostris) embryos. Acta Oceano. Sin., 25(4): 91–98.

    Google Scholar 

  • Chiu Y N, Benitez L V. 1981. Studies on the carbohydrases in the digestive tract of the milkfish Chanos chanos. Mar. tBiol., 61:247–254.

    Article  Google Scholar 

  • De Almeida L C, Lundstedt L M, Moraes G. 2006. Digestive enzyme responses of tambaqui (Colossoma macropomum) fed on different levels of protein and lipid. Aquacult. Nutr., 12: 443–450.

    Article  Google Scholar 

  • Deguara S, Jauncey K, Agius C. 2003. Enzyme activities and pH variations in the digestive tract of gilthead sea bream. J. Fish. Biol., 62: 1 033–1 043.

    Article  Google Scholar 

  • Fernandez I, Moyano F J, Diaz M, Martinez T. 2001. Characterization of α-amylase activity in five species of Mediterranean sparid fishes (Sparidae, Teleostei). J. Exp. Mar. Biol. Ecol., 262: 1–12.

    Article  Google Scholar 

  • Furne M, Hidalgo M C, Lopez A, Garcia-Gallego M, Morales A E, Domezain A, Domezaine J, Sanz A. 2005. Digestive enzyme activities in Adriatic sturgeon Acipenser naccarii and rainbow trout Oncorhynchus mykiss. A comparative study. Aquacult., 250: 391–398.

    Article  Google Scholar 

  • German D P, Horn M H, Gawlicka A. 2004. Digestive enzyme activities in herbivorous and carnivorous Prickleback fishes (Teleostei: Stichaeidae): ontogenetic, dietary, and phylogenetic effects. Physiol. Biochem. Zool., 77: 789–804.

    Article  Google Scholar 

  • Hakim Y, Uni Z, Hulata G. 2006. Relationship between intestinal brush border enzymatic activity and growth rate in tilapias fed diets containing 30% or 48% protein. Aquacult., 257: 420–428.

    Article  Google Scholar 

  • Hakim Y, Rowland S J, Guy J A, Mifsud C, Uni Z, Harpaz S. 2007. Effects of genetic strain and holding facility on the characteristics of alkaline phosphatase and brush border enzymes in silver perch (Bidyanus bidyanus). Aquacult. Res., 38: 361–372.

    Article  Google Scholar 

  • Hirji K N, Courtney W A M. 1982. Lecuine aminopeptidase activity in the digestive tract of perch, Perca fluviatilis L. J. Fish Biol., 21: 615–622.

    Article  Google Scholar 

  • Hidalgo M C, Urea E, Sanz A. 1999. Comparative study of digestive enzymes in fish with different nutritional habits. Proteolytic and amylase activities. Aquacult., 170: 267–283.

    Article  Google Scholar 

  • Hofer R. 1982. Protein digestion and proteolytic activity in the digestive tract of an omnivorous cyprinid. Comp. Biochem. Physiol., A72: 55–63.

    Article  Google Scholar 

  • Hong W S, Zhang Q Y. 2003. Review of captive bred species and fry production of marine fish in China. Aquacult., 227: 305–318.

    Article  Google Scholar 

  • Iijima N, Tanaka S, Ota Y. 1998. Purification and characterization of bile salt-activated lipase from the hepatopancreas of red sea bream, Pagrus major. Fish Physiol. Biochem., 18:59–69.

    Article  Google Scholar 

  • Izquierdo M S, Socorro J, Arantzamendi L. 2000. Recent advances in lipid nutrition in fish larvae. Fish Physiol. Biochem., 22: 97–107.

    Article  Google Scholar 

  • Kapoor B G, Smith H, Verighina I A. 1975. The alimentary canal and digestion in teleosts. Adv. Mar. Biol., 13: 109–211.

    Article  Google Scholar 

  • Karasov W H. 1992. Tests of the adaptive modulation hypothesis for dietary control of intestinal nutrient transport. Am. J. Physiol., 263: 496–502.

    Google Scholar 

  • Kuz’mina V V. 1996. Influence of age on digestive enzyme activity in some freshwater teleosts. Aquacult., 148: 25–37.

    Article  Google Scholar 

  • Kuz’mina V V, Golovanova I L, Izvekova G I. 1996. Influence of temperature and season on some characteristics of intestinal mucosa carbohydrases in six freshwater fishes. Comp. Biochem. Physiol., B113: 255–260.

    Google Scholar 

  • Munilla-Moran R, Saborido-Rey F. 1996. Digestive enzymes in marine species. II. Amylase activities in gut from seabream (Sparus aurata), turbot (Scophthalmus maximus) and redfish (Sebastes mentella). Comp. Biochem. Physiol., B113: 827–834.

    Google Scholar 

  • Sabapathy U, Teo L H. 1993. A quantitative study of some digestive enzymes in the rabbitfish, Siganus canaliculatus and the sea bass, Lates calcarifer. J. Fish Biol., 42: 595–602.

    Article  Google Scholar 

  • Tengjaroenkul B, Smith B J, Caceci T, Smith S A. 2000. Distribution of intestinal enzyme activities along the intestinal tract of cultured Nile tilapia, Oreochromis niloticus L. Aquacult., 182: 317–327.

    Article  Google Scholar 

  • Uys W, Hecht T. 1987. Assays on the digestive enzymes of sharptooth catfish, Clarias gariepinus (Pisces: Clariidae). Aquacult., 63: 301–313.

    Article  Google Scholar 

  • Wang H T, Zhang P J. 2002. Activities of digestive enzyme in differen tissues of Paralichthys olivaceus. Oceanol. Limnol. Sin., 33(5): 473–476. (in Chinese with English abstract)

    Google Scholar 

  • Watanabe T. 1982. Lipid nutrition in fish. Comp. Biochem. Physiol., B73: 3–15.

    Google Scholar 

  • Wu R X, Hong W S, Zhang Q Y, Ge W, He Z B. 2007. Studies on the activities of digestive enzymes of Chinese black sleeper (Bostrichtys sinensis). J. Xiamen Univ. (Natural Science), l46(1): 118–122. (in Chinese with English abstract)

    Google Scholar 

  • Zambonino Infante J L, Cahu C L. 2001. Ontogeny of the gastrointestinal tract of marine fish larvae. Comp. Biochem. Physiol., A130: 477–487.

    Google Scholar 

  • Zhu A Y, Chu X L. 2006. Activity and distribution of two enzymes in different parts of digestive tract of Pseudosciaena crocea: temperature and pH impacts. Oceanol. Limnol. Sin., 37(6): 561–567. (in Chinese with English abstract)

    Google Scholar 

  • Zhu Y F, Zhang Q Y. 1993. On feeding habits and histological structure of digestive tract of the mudskipper, Boleophthalmus pectinirostris, in intertidal zone of Jiulong River Estuary. J. Ocean Taiwan Strait, 12(3): 225–232. (in Chinese with English abstract)

    Google Scholar 

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Authors and Affiliations

  1. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361005, China

    Renxie Wu  (吴仁协), Wanshu Hong  (洪万树) & Qiyong Zhang  (张其永)

  2. College of Fisheries, Guangdong Ocean University, Zhanjiang, 524088, China

    Renxie Wu  (吴仁协)

Authors
  1. Renxie Wu  (吴仁协)
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  2. Wanshu Hong  (洪万树)
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  3. Qiyong Zhang  (张其永)
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Corresponding author

Correspondence to Wanshu Hong  (洪万树).

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Supported by Natural Science Foundation of China (No. 40476056) and the Key Projects of Science and Technology of Fujian Province (2003, No. 26; 2004SZ01-02)

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Wu, R., Hong, W. & Zhang, Q. Digestive enzyme activities in mudskipper Boleophthalmus pectinirostris and Chinese black sleeper Bostrichthys sinensis . Chin. J. Ocean. Limnol. 28, 756–761 (2010). https://doi.org/10.1007/s00343-010-9111-5

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  • DOI: https://doi.org/10.1007/s00343-010-9111-5

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