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Anti-proliferative activity of phlorotannin extracts from brown algae Laminaria japonica Aresch

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Abstract

In this study, we evaluated the anti-proliferative activity of phlorotannins derived from brown algae Laminaria japonica Aresch extracts on the human hepatocellular carcinoma cell (BEL-7402) and on murine leukemic cells (P388) by MTT assay. Cells were incubated with 100 μg/mL of the phlorotannin extract (PE) for 48 h. The inhibitory rate of PE on BEL-7402 and P388 cells was 30.20±1.16% and 43.44±1.86%, respectively, and the half-inhibitory concentration of PE (IC50) on P388 and BEL-7402 cells was 120 μg/mL and >200 μg/mL, respectively. Microscopic observation shows that the morphologic features of tumor cells treated with PE and 5-fluorouracil are markedly different from the normal control group. The inhibitory rate of fraction A2 isolated from PE by sephadex LH-20 for BEL-7402 and P388 cells at the sample concentration of 70.42 μg/mL was 61.96±7.02% and 40.47±8.70%, respectively. The apoptosis peak for fraction A2 was the most profound of all fractions used in the flow cytometry assay. The results indicate that the anti-proliferative of this algal extract is associated with the total phlorotannin content.

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Authors and Affiliations

  1. College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China

    Huicheng Yang  (杨会成), Mingyong Zeng  (曾名湧), Shiyuan Dong  (董士远), Zunying Liu  (刘尊英) & Ruixue Li  (李瑞雪)

  2. Zhejiang Marine Development Research Institute, Zhoushan, 316100, China

    Huicheng Yang  (杨会成)

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  1. Huicheng Yang  (杨会成)
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  2. Mingyong Zeng  (曾名湧)
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  3. Shiyuan Dong  (董士远)
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  4. Zunying Liu  (刘尊英)
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  5. Ruixue Li  (李瑞雪)
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Correspondence to Mingyong Zeng  (曾名湧).

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Supported by the National Key Technology Research & Development Program of the 11th Five Year Plan of China (No. 2006BAD30B01) and the National Natural Science Foundation of China (No. 30871945)

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Yang, H., Zeng, M., Dong, S. et al. Anti-proliferative activity of phlorotannin extracts from brown algae Laminaria japonica Aresch. Chin. J. Ocean. Limnol. 28, 122–130 (2010). https://doi.org/10.1007/s00343-010-9054-x

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  • DOI: https://doi.org/10.1007/s00343-010-9054-x

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