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Characteristics of the tertiary egg membrane of cuttlefish Sepiella maindroni de Rochebrune

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Abstract

We separated tertiary egg membrane (TGM) from 2- and 25-day-old eggs of cuttlefish Sepiella maindroni de Rochebrune, and revealed its ultrastructure, physical (solubility, barrier property) and biochemical (histology, histochemistry, nutritional components, bacteriostasis) characteristics. The results show that TGM could not be dissolved with natural seawater, alcohol, ether or hydrochloric acid (HCl), but it could be dissolved with 2-chloroethanol, diethylamine, and sodium hydroxide (NaOH). The black TGM was more effective in blocking off mud particulates, microorganisms (Chlorella vulgaris, Vibrio alginolyticus) and lighter than the white TGM. The elasticity of black and white TGMs was 1.8 N and 1.5 N, respectively. There were some ink particulates and rod-shaped bacteria in the black TGM. The nutritional components were different between black and white TGMs: Lipid content was lower and protein content was higher in the black TGM. TGM could also inhibit the growth of Vibrio alginolyticus.

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Authors and Affiliations

  1. Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, 315211, China

    Chunlin Wang  (王春琳), Xiaoxu Fan  (樊晓旭), Xiamin Jiang  (蒋霞敏), Weiwei Song  (宋微微) & Yongjian Xu  (徐永健)

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  1. Chunlin Wang  (王春琳)
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  2. Xiaoxu Fan  (樊晓旭)
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  3. Xiamin Jiang  (蒋霞敏)
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  4. Weiwei Song  (宋微微)
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  5. Yongjian Xu  (徐永健)
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Corresponding author

Correspondence to Yongjian Xu  (徐永健).

Additional information

Supported by the National Natural Science Foundation of China (No. 40646030, 40776076), the Changjiang River Scholar and Innovative Research Team in university (No. IRT0734), Key Scientific Research Project of Education Ministry of China (No.207045), Key Project of Science and Technology of Zhejiang Province (No. 2006C13040; 2007C12076; 2009R10012) and K.C. Wong Magna Fund in Ningbo University

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Wang, C., Fan, X., Jiang, X. et al. Characteristics of the tertiary egg membrane of cuttlefish Sepiella maindroni de Rochebrune. Chin. J. Ocean. Limnol. 28, 1206–1212 (2010). https://doi.org/10.1007/s00343-010-9024-3

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  • DOI: https://doi.org/10.1007/s00343-010-9024-3

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