Abstract
Pen a 1 is a major shrimp allergen that induces an allergy. The aim of this study is to assess the effect of simulated gastrointestinal fluids on the allergenicity of Pen a 1. Purified Pen a 1 from shrimp (Penaeus vannamei) was subjected to digestion in SGF (simulated gastric fluid) and SIF (simulated intestinal fluid) for a set time. The allergenicity of digestive Pen a 1 was analyzed by immunoblotting and Ci-ELISA, using pool sera from patients with shrimp specific IgE. The results showed that Pen a 1 exhibited a decrease in allergenicity with increasing digestion time in the SGF and SIF. However, Pen a l exhibited strong resistance to digestive fluids, and all yielded fragments (33 kD, 23 kD, and 14 kD) showed allergic activity. Therefore, anti-digestion may be an important factor for Pen a 1 to induce an allergy.
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Supported by the National High Technology Research and Development Program of China (863 Program) (NO.2006AA09Z427) and the National Natural Science Foundation of China (Nos. 30800859, 30871948)
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Guo, Y., Li, Z. & Lin, H. The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity. Chin. J. Ocean. Limnol. 27, 703–707 (2009). https://doi.org/10.1007/s00343-009-9190-3
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DOI: https://doi.org/10.1007/s00343-009-9190-3