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Polypeptide from Chlamys farreri inhibits UVB-induced apoptosis of HaCaT cells via iNOS/NO and HSP90

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Abstract

Polypeptide from Chlamys farreri (PCF) is a novel marine bioactive product that was isolated from the gonochoric Chinese scallop Chlamys farreri, and was found to be an effective antioxidant in our recent studies. In this study, we investigated the effect of PCF on ultraviolet B (UVB)-induced apoptosis of HaCaT cells and the intracellular signaling pathways involved. Pretreatment with the inducible nitric oxide synthase (iNOS) inhibitor S-methylisothiourea sulfate inhibited UVB-induced apoptosis, indicating that iNOS and NO play important roles in apoptosis. On the other hand, the inhibition of UVB-induced apoptosis in the immortalized keratinocyte (HaCaT) cells by PCF was estimated using a DNA ladder. PCF treatment inhibited UVB-induced iNOS activation, as determined by RT-PCR, NO production, as determined by ESR, and up-regulated heat shock protein (HSP) 90 activation, as determined by Western blotting. Our results indicate that iNOS and NO are involved in UVB-induced apoptosis of HaCaT cells and the protective effect of PCF against UVB irradiation is exerted by suppressing the expression of iNOS, followed by inhibition of NO release and enhanced activation of HSP90.

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Authors and Affiliations

  1. Department of Pharmacology, Medical College of Qingdao University, Qingdao, 266071, China

    Zhengyang Zhang  (张正洋), Xiaojin Liu  (刘小金), Lin Yan  (闫琳) & Chunbo Wang  (王春波)

  2. Department of Public Health, Medical College of Qingdao University, Qingdao, 266071, China

    Tuo Liu  (刘拓)

  3. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China

    Yuejun Wang  (王跃军)

Authors
  1. Zhengyang Zhang  (张正洋)
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  2. Xiaojin Liu  (刘小金)
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  3. Tuo Liu  (刘拓)
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  4. Lin Yan  (闫琳)
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  5. Yuejun Wang  (王跃军)
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  6. Chunbo Wang  (王春波)
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Corresponding author

Correspondence to Zhengyang Zhang  (张正洋).

Additional information

Supported by the National Natural Science Foundation of China (30471458) and National Natural Science Foundation of Shandong Province (Z2007c09)

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Zhang, Z., Liu, X., Liu, T. et al. Polypeptide from Chlamys farreri inhibits UVB-induced apoptosis of HaCaT cells via iNOS/NO and HSP90. Chin. J. Ocean. Limnol. 27, 594–599 (2009). https://doi.org/10.1007/s00343-009-9171-6

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  • DOI: https://doi.org/10.1007/s00343-009-9171-6

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