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Optimization of conjugated linoleic acid triglycerides via enzymatic esterification in no-solvent system

  • Chemistry
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Abstract

We compared four esterifiable enzymes. The lipase Novozym 435 possessed the highest activity for the conjugated linoleic acid esterification during the synthesis of triglycerides. The triglycerides were synthesized by esterification of glycerol and conjugated linoleic acid (CLA) in a no-solvent system using lipase catalysis. We investigated the effects of temperature, enzyme concentration, water content, and time on esterification. Enzyme and water concentrations of up to 1% of the total reaction volume and a system temperature of 60°C proved optimal for esterification. Similarly, when the esterification was carried out for 24 h, the reaction ratio improved to 94.11%. The esterification rate of the rotating screen basket remained high (87.28%) when the enzyme was re-used for the 5th time. We evaluated the substrate selectivity of lipase (NOVO 435) and determined that this lipase prefers the 10,12-octadacadienoic acid to the 9,11-octadecadienoic acid.

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References

  • Anna M F, Patrick A, Mattiasson B. 1996. Glyceride synthesis in a solvent-free system. J. Am. Oil Chem. Soc., 73: 1 489–1 495.

    Google Scholar 

  • Ha Y L, Grimm NK, Pariza M W. 1987. Anticarcinogens from field ground beef: heat-altered derivatives of linoleic acid. Carcinogenesis, 8: 1 881–1 887.

    Article  Google Scholar 

  • Ip C, Chin S F, Scimeca J A, Pariza M W. 1991. Mammary cancer prevention by conjugated dienoic derivatives of linoleic acid. Cancer Res., 51: 6 118–6 124.

    Google Scholar 

  • Lee KN, Kritchevsky D, Pariza M W. 1994. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis, 108: 19–25.

    Article  Google Scholar 

  • Nicolosi R J, Roders E J, Kritchevsky D, Scimeca J A, Huth P J. 1997. Dietary conjugated linoleic acid deduces plasma lipoproteins and early aortic atherosclerosis in hypercholesterolemic hamsters. Artery, 22: 266–277.

    Google Scholar 

  • Ning Z X. 1998. Handbook of Food Ingredients (The first edition). China Light Industry Publishing House, 186–188. (in Chinese)

  • Ostrowska E, Muralitharan M, Cross R F, Bauman D E, Dunshea F R. 1999. Dietary conjugated linoleic acids increase lean tissue and decrease fat deposition in growing pigs. J. Nutri., 129: 2 037–2 042.

    Google Scholar 

  • Pariza M W. 1991. A new cancer inhibitor in dairy products. Bull. Int. Dairy Fed., 257: 29–30.

    Google Scholar 

  • Park Y, Albright K J, Liu W, Storkson J M, Cook M E, Pariza M W. 1997. Effect of conjugated linoleic acid on body composition in mice. Lipids, 32: 853–858.

    Article  Google Scholar 

  • Rahman S M, Wang Y M, Yotsumoto H, Cha J Y, Han S Y, Inoue S, Yanagita T. 2001. Effect of conjugated linoleic acid on serum leptin concentration, body fat accumulation, and beta-oxidation of fatty acid in OLETF rats. Nutrition, 17: 385–390.

    Article  Google Scholar 

  • Sugano M, Tsujita A, Yamasaki M, Noguchi M, Yamada K. 1998. Conjugated linoleic acid modulates tissue levels of chemical mediators and immunoglobulins in rats. Lipids, 33: 521–527.

    Article  Google Scholar 

  • Warwel S, Borydorf R. 2000. Substrate selectivity of lipases in the esterification of cis/trans-isomers and positional isomers of conjugated linoleic acid (CLA). Biotechnology Letters, 22: 1 151–1 155.

    Article  Google Scholar 

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Authors and Affiliations

  1. College of Life Sciences, Ocean University of China, Qingdao, 266003, China

    Dan Yi  (衣丹)

  2. Key Laboratory of Marine Bio-active Substance, First Institute of Oceanography, State Oceanic Administration, Qingdao, 266061, China

    Dan Yi  (衣丹), Xiuqin Sun  (孙修勤), Guangyou Li  (李光友), Fayi Liu  (刘发义), Xuezheng Lin  (林学政) & Jihong Shen  (沈继红)

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  1. Dan Yi  (衣丹)
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  2. Xiuqin Sun  (孙修勤)
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  3. Guangyou Li  (李光友)
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  4. Fayi Liu  (刘发义)
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  5. Xuezheng Lin  (林学政)
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  6. Jihong Shen  (沈继红)
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Corresponding author

Correspondence to Xiuqin Sun  (孙修勤).

Additional information

Supported by the High Technology Research and Development Program of China (No. 2007AA091905) and the Technical Program of Municipal Technology Bureau Qingdao (No. 04-2-JS-117)

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Yi, D., Sun, X., Li, G. et al. Optimization of conjugated linoleic acid triglycerides via enzymatic esterification in no-solvent system. Chin. J. Ocean. Limnol. 27, 574–577 (2009). https://doi.org/10.1007/s00343-009-9170-7

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  • DOI: https://doi.org/10.1007/s00343-009-9170-7

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