Skip to main content
SpringerLink
Log in

Inhibitory effects and mechanisms of high molecular-weight phlorotannins from Sargassum thunbergii on ADP-induced platelet aggregation

  • Chemistry
  • Published:
Chinese Journal of Oceanology and Limnology Aims and scope Submit manuscript

Abstract

We evaluated the effects of high molecular-weight phlorotannins from Sargassum thunbergii (STP) on ADP-induced platelet aggregation and arachidonic acid (AA) metabolism in New Zealand white rabbits and Wistar rats. The inhibition of STP on platelet aggregation was investigated using a turbidimetric method, and the levels of the terminal products of AA metabolism were measured using the corresponding kits for maleic dialdehyde (MDA), thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-keto-PGF1α) by colorimetry and radioimmunoassay, as appropriate. We found that STP could inhibit ADP-induced platelet aggregation, and the inhibitory ratio was 91.50% at the STP concentration of 4.0 mg/mL. Furthermore, STP markedly affected AA metabolism by decreasing the synthesis of MDA (P<0.01) and increasing the synthesis of 6-keto-PGF1α, thus changing the plasma TXB2/6-keto-PGF1α balance when the platelets were activated (P<0.01). Therefore, STP altered AA metabolism and these findings partly revealed the molecular mechanism by which STP inhibits ADP-induced platelet aggregation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Brianne E H, Kathryn V A. 2004. Effects Of UV radiation on growth and phlorotannins in Fucus gardneri (Phaeophyceae) juveniles and embryos. J. Phycol., 40: 527–533.

    Article  Google Scholar 

  • Eric N J, Lawrence F B, Colin D F. 1998. Increased platelet sensitivity to ADP in mice lacking platelet-type 12-lipoxygenase. Proc. Natl. Acad. Sci., 95: 3 100–3 105.

    Google Scholar 

  • Gachet C. 2001. ADP receptors of platelets and their inhibition. Thromb Haemost, 86: 222–232.

    Google Scholar 

  • Greco N J, Tonon G, Chen W, Luo X, Dalal R, Jamieson G A. 2001. Novel structurally altered P2X1 receptor is preferentially activated by adenosine diphosphate in platelets and megakaryocytic cells. Blood, 98: 100–107.

    Article  Google Scholar 

  • Jiang F W, Zhang Y S. 1993. Chinese Dictionary of Marine Drugs. Ocean Press of China, Beijing. p387 (in Chinese)

    Google Scholar 

  • Lagarde M, Gualde N, Rigaud M. 1989. Metabolic interactions between eicosanoids in blood and vascular cells. Biochem. J., 257(2): 313–320

    Google Scholar 

  • Li J, Wei Y X, Du G C, Hu Y F, Li L. 2007. Anticoagulant activities of phlorotannins from Sargassum thunbergii Kuntze. Traditional Chinese Drug Research & Clinical Pharmacology, 18(3): 191–194. (in Chinese)

    Google Scholar 

  • Lin C, Yu S G, Guo D S, Wei Y X, Ai G H. 2006. The antibacterial activity of phlorotannins from Sargassum thunbergii kuntze. Mar. Sci., 30(3): 94–97. (in Chinese)

    Google Scholar 

  • Ma Y, Xu Q P, Sun J N. 1999. Antagonistic effects of shikimic acid against focal cerebral ischemia injury in rats subjected to middle cerebral artery thrombosis. Acta Pharmacologica Sinica, 20(8): 701–704.

    Google Scholar 

  • Marcus A J, Weksler B B, Jaffe E A, Broekman M J. 1980. Synthesis of prostacyclin from platelet-derived endoperoxides by cultured human endothelial cells. J. Clin. Invest., 66(5): 979–986.

    Article  Google Scholar 

  • McInnes A G, Ragan M A, Smith D G, Walter J A. 1985. The high molecular weight polyphloroglucinols of the marine brown alga Fucus vesiculosus L. 1H and 13C nuclear magnetic resonance spectroscopy. Can. J. Chem., 63: 304–313.

    Article  Google Scholar 

  • Ohlmann P, Eckly A, Freund M, Cazenave J P, Offermanns S, Gachet C. 2000. ADP induces partial platelet aggregation without shape change and potentiates collagen-induced aggregation in the absence of Galphaq. Blood, 96: 2 134–2 139.

    Google Scholar 

  • Samuel W C, Steven J G, Byung K K, Mao J G, Michael G B, Paul C Z. 1997. P1, P4-dithio-P2,P3-monochloromethylene diadenosine 5′,5‴-P1,P4-tetraphosphate: a novel antiplatelet agent. Proc. Natl. Acad. Sci., 94: 4 034–4 039.

    Google Scholar 

  • Swain T, Hillis W E. 1959. The phenolic constituents of Prunus domestica. I. The quantitative analysis of phenolic constituents. J. Sci. Food Agric., 10: 63–68.

    Article  Google Scholar 

  • Targett N M, Arnold T M. 1998. The role of algal phlorotannins in marine plant-herbivore interactions. J. Phycol., 34(2): 195–205.

    Article  Google Scholar 

  • Tolhurst G, Vial C, Leon C, Gachet C, Evans R J, Mahaut-Smith M P. 2005. Interplay between P2Y1, P2Y12, and P2X1 receptors in the activation of megakaryocyte cation influx currents by ADP: evidence that the primary megakaryocyte represents a fully functional model of platelet P2 receptor signaling. Blood, 106: 1 644–1 651.

    Article  Google Scholar 

  • Shibata T, Nagayama K, Tanaka R, Yamaguchi K, Nakamura T. 2003. Inhibitory effects of brown algal phlorotannins on secretory phospholipase A2s, lipoxygenases and cyclooxygenases. J. Appl. Phycol., 15(11): 61–66.

    Article  Google Scholar 

  • Vargaftig B B, Chignard M, Benveniste J. 1981. Present concepts on the mechanisms of platelet aggregation. Biochem. Pharmacol., 30: 263–271.

    Article  Google Scholar 

  • Wei Y X, Li J, Wang J C, Qi H T. 2007. Screening of different fractions of phlorotannins from Sargassum thunbergii kuntze for anticoagulant activity. Chin. J. Biochem. Pharmaceutics, 28(4): 227–229. (in Chinese)

    Google Scholar 

  • Wei Y X, Li Z E, Hu Y F, Xu Z H. 2003. Inhibition of mouse liver lipid peroxidation by high molecular weight phlorotannins from Sargassum kjellmanianum. J. Appl. Phycol., 15(6): 507–511.

    Article  Google Scholar 

  • Xu S Y, R L Bian, Chen X. 2002. Methodology of Pharmacological Experiment (Third edition). People Public Health Publishing Company, Beijing. p. 1 281–1 283. (in Chinese)

    Google Scholar 

  • Yan X J, Li X C, Zhou C X, Fan X. 1996. Prevention of fish oil rancidity by phlorotannins from Sargassum kjellmanianum. J. Appl. Phycol., 8: 201–203.

    Article  Google Scholar 

  • Zucker M B, Nachmias V T. 1985. Platelet activation. Arteriosclerosis, 5: 2–18.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biological Department, Qingdao University, Qingdao, 266071, China

    Yuxi Wei  (魏玉西), Qi Guo  (郭奇) & Hongtao Qi  (齐宏涛)

  2. Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, China

    Changyun Wang  (王长云)

  3. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Jing Li  (李敬)

Authors
  1. Yuxi Wei  (魏玉西)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Changyun Wang  (王长云)
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jing Li  (李敬)
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Qi Guo  (郭奇)
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hongtao Qi  (齐宏涛)
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Yuxi Wei  (魏玉西) or Changyun Wang  (王长云).

Additional information

Supported by the National Natural Science Foundation of China (No. 30572314), the Basic Research Program of Science and Technology, Ministry of Science and Technology of China (2007FY210500), the Program of Chinese Offshore Investigation and Assessment, State Oceanic Administration of China (Nos. 908-01-ST12 and 908-02-05-04), and the Science and Technology Planning Project of Qingdao (No. 06-2212-JCH)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wei, Y., Wang, C., Li, J. et al. Inhibitory effects and mechanisms of high molecular-weight phlorotannins from Sargassum thunbergii on ADP-induced platelet aggregation. Chin. J. Ocean. Limnol. 27, 558–563 (2009). https://doi.org/10.1007/s00343-009-9166-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00343-009-9166-3

Keyword

Search

Navigation